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1. 发明授权

EP3013962B1 EXPRESSION OF NATIVELY SECRETED POLYPEPTIDES WITHOUT SIGNAL PEPTIDE 有权
公开(公告)号：EP3013962B1
公开(公告)日：2018-10-10
申请号：EP14732156.6
申请日：2014-06-18
申请人： Novozymes A/S
发明人： BORCHERT, Martin, Simon
IPC分类号： C12N15/67 , C12N9/52
CPC分类号： C12P21/02 , C12N9/248 , C12N9/2482 , C12N9/52 , C12N15/67 , C12N15/75 , C12Y302/01008 , C12Y304/21062
摘要： The present invention relates to methods of recombinantly producing a natively secreted polypeptide, the method comprising the steps of providing a microorganism host cell comprising an exogenous polynucleotide encoding a natively secreted polypeptide without a translationally fused signal peptide; cultivating the microorganism host cell under conditions conducive to the expression of the polypeptide and, optionally, recovering the polypeptide, as well as microorganisms, certain polynucleotides, expression constructs and protease substitution variants.

2. 发明授权

EP1992698B1 METHOD FOR SYNTHESIS OF SUPPRESSOR tRNA, DNA CONSTRUCT, AND PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE DNA CONSTRUCT 有权
公开(公告)号：EP1992698B1
公开(公告)日：2018-09-05
申请号：EP07714802.1
申请日：2007-02-22
申请人： Riken
发明人： YOKOYAMA, Shigeyuki , SAKAMOTO, Kensaku , HINO, Nobumasa , MUKAI, Takahito , KOBAYASHI, Takatsugu
IPC分类号： C12P19/34 , C12P21/02 , C12N15/67 , C12N15/85 , C12P21/00
CPC分类号： C12N15/85 , C12N15/67 , C12P19/34 , C12P21/00 , C12P21/02
摘要： There are provided a DNA construct comprising non-eukaryote-derived suppressor tRNA gene containing no internal promoter functioning in a eukaryotic cell, and a eukaryote-derived or bacteriophage-derived promoter linked at the 5' terminal of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing a non-natural amino acid-incorporated protein by using the same.

3. 发明公开

EP3360964A1 CRISPR-BASED GENOME MODIFICATION AND REGULATION 审中-公开
公开(公告)号：EP3360964A1
公开(公告)日：2018-08-15
申请号：EP18156734.8
申请日：2013-12-05
申请人： Sigma-Aldrich Co. LLC
发明人： Chen, Fuqiang , Davis, Gregory D.
IPC分类号： C12N15/10 , C12N15/63 , C12N9/22
CPC分类号： C12N15/907 , A61K38/00 , C07K7/06 , C07K14/463 , C07K2319/09 , C07K2319/10 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N9/96 , C12N15/102 , C12N15/11 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/86 , C12N2310/3513 , C12N2750/14143 , C12N2800/22 , C12N2800/80 , C12Y301/00 , C12Y301/21004
摘要： The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

4. 发明公开

EP3218483A4 COMPOUNDS AND METHODS FOR THE MODULATION OF PROTEINS 审中-公开
公开(公告)号：EP3218483A4
公开(公告)日：2018-07-18
申请号：EP15859580
申请日：2015-11-16
申请人： IONIS PHARMACEUTICALS INC
发明人： CROOKE STANLEY T , LIANG XUE-HAI , SHEN WEN
IPC分类号： C07H21/00 , A61K31/7088 , C12N15/113 , C12P19/34
CPC分类号： C07H21/04 , A61K31/7088 , C07H21/00 , C12N15/113 , C12N15/1137 , C12N15/1138 , C12N15/67 , C12N2310/11 , C12N2310/315 , C12N2310/3341 , C12N2310/346 , C12P19/34 , C12Y301/26004 , C12N2310/321 , C12N2310/3521 , C12N2310/3525
摘要： In certain embodiments, the present disclosure provides compounds and methods of increasing the amount or activity of a target protein in a cell. In certain embodiments, the compounds comprise a translation suppression element inhibitor. In certain embodiments, the translation suppression element inhibitor is a uORF inhibitor. In certain embodiments, the uORF inhibitor is an antisense compound.

5. 发明公开

EP3337902A2 3' UTR SEQUENCES FOR STABILIZATION OF RNA 审中-公开
公开(公告)号：EP3337902A2
公开(公告)日：2018-06-27
申请号：EP16778784.5
申请日：2016-10-05
申请人： BioNTech RNA Pharmaceuticals GmbH , TRON - Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg- Universität Mainz gemeinnützige GmbH
发明人： ORLANDINI VON NIESSEN, Alexandra , FESSER, Stephanie , VALLAZZA, Britta , BEISSERT, Tim , KUHN, Andreas , SAHIN, Ugur , POLEGANOV, Marco Alexander
IPC分类号： C12N15/67
CPC分类号： C12N15/67
摘要： The present invention relates to stabilization of RNA, in particular mRNA, and an increase in mRNA translation. The present invention particularly relates to a modification of RNA, in particular in vitro-transcribed RNA, resulting in increased transcript stability and/or translation efficiency. According to the invention, it was demonstrated that certain sequences in the 3'- untranslated region (UTR) of an RNA molecule improve stability and translation efficiency.

6. 发明公开

EP3332009A1 METHODS OF SCREENING FOR RIBOSWITCHES AND ATTENUATORS 审中-公开
公开(公告)号：EP3332009A1
公开(公告)日：2018-06-13
申请号：EP16754565.6
申请日：2016-08-02
申请人： Yeda Research and Development Co., Ltd.
发明人： SOREK, Rotem , DAR, Daniel
IPC分类号： C12N15/115 , C12N15/67
CPC分类号： C12N15/113 , C07H21/02 , C12N15/115 , C12N15/67 , C12Q1/04 , G01N33/582
摘要： A method of determining a transcription termination site in bacterial DNA is disclosed. Uses of sequences comprising transcription termination sites are also disclosed.

7. 发明公开

EP3325622A1 A SYSTEM, DEVICE AND A METHOD FOR PROVIDING A THERAPY OR A CURE FOR CANCER AND OTHER PATHOLOGICAL STATES 审中-公开
公开(公告)号：EP3325622A1
公开(公告)日：2018-05-30
申请号：EP16742294.8
申请日：2016-07-22
申请人： Frost, Habib
发明人： Frost, Habib
IPC分类号： C12N15/10 , C12N9/22 , G06Q50/00
CPC分类号： A61K48/0066 , A61P35/00 , C12N9/22 , C12N15/102 , C12N15/64 , C12N15/67 , G06F19/18 , G06F19/22 , C12Q2521/301 , C12Q2539/101
摘要： Provided is a method for treatment of pathologies caused by cells that have DNA sequences that differ from DNA of healthy human cells. The method utilises targeting of the sequence differences and effects cleavage of the DNA which does not occur in the healthy genome, whereby the cells containing the targeted DNA are killed or inactivated. Preferred systems for effecting such DNA damage is CRISPR systems where a CAS cleaves DNA adjacent to a sequence recognized by the CRISPR. Also disclosed are methods and a computer system for designing and/or producing the targeting nucleic acids.

8. 发明授权

EP2970949B1 NOVEL NUCLEIC ACID MOLECULES 有权
公开(公告)号：EP2970949B1
公开(公告)日：2018-05-30
申请号：EP14711216.3
申请日：2014-03-14
申请人： MedImmune Limited
发明人： DIECI, Giorgio , MARELLI, Marcello , GRABSTEIN, Kenneth H.
IPC分类号： C12N15/09 , C12N15/11
CPC分类号： C12N15/85 , C12N15/67 , C12N2800/107 , C12P21/00
摘要： There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system.

9. 发明公开

EP3317415A1 UTRs INCREASING THE TRANSLATION EFFICIENCY OF RNA MOLECULES 审中-公开
标题翻译： UTRs提高RNA分子的翻译效率
公开(公告)号：EP3317415A1
公开(公告)日：2018-05-09
申请号：EP16742171.8
申请日：2016-06-30
申请人： Ethris GmbH
发明人： RUDOLPH, Carsten , ANEJA, Manish Kumar , FERIZI, Mehrije , GEIGER, Johannes
IPC分类号： C12N15/67
CPC分类号： C12N15/67
摘要： Described is an RNA molecule comprising (a) a coding region coding for a polypeptide; and (b) upstream of said coding region one or more UTR(s) comprising the sequence as shown in SEQ ID NO:1 or a sequence which shows 1 to 4 substitutions in comparison to SEQ ID NO:1 and which results in an RNA molecule having the same or a higher translation efficiency as an RNA molecule comprising an UTR comprising SEQ ID NO:1; and/or (c) downstream of said coding region one or more UTR(s) comprising the sequence as shown in SEQ ID NO:2 or a sequence which shows 1 to 7 substitutions in comparison to SEQ ID NO:2 and which results in an RNA molecule having the same or a higher translation efficiency as an RNA molecule comprising an UTR comprising SEQ ID NO:2; wherein said polypeptide encoded by said coding region is not a cytochrome b-245 alpha polypeptide (CYBA). Moreover, described is a nucleic acid molecule encoding the RNA molecule according to the present invention. Further, described is a vector comprising the nucleic acid molecule according to the present invention and to a host cell comprising the vector according to the present invention. Further, described is a pharmaceutical composition comprising the RNA molecule according to the present invention and optionally a pharmaceutically acceptable carrier. Moreover, described is a kit comprising the RNA molecule according to the present invention. Finally, described is the use of one or more UTR(s) as defined in (b) and/or one or more UTR(s) as defined in (c) for increasing the efficiency of translating a coding region of an RNA molecule into a polypeptide or a protein encoded by said coding region.

10. 发明公开

EP3313980A1 ALGAL PROMOTERS 审中-公开
公开(公告)号：EP3313980A1
公开(公告)日：2018-05-02
申请号：EP16813855.0
申请日：2016-06-23
申请人： B.G. Negev Technologies and Applications Ltd., at Ben-Gurion University
发明人： BOUSSIBA, Sammy , KHOZIN-GOLDBERG, Inna , SHEMESH, Zachor , LEU, Stefan , ZARKA, Aliza
IPC分类号： C12N1/13 , C12N15/63 , C12N15/113
CPC分类号： C12N15/67 , C12N15/63
摘要： The present invention provides a nucleic acid molecule comprising: DGAT1 promoter or an active fragment thereof, the ammonium transporter promoter or an active fragment thereof, or the putative purine permease promoter or an active fragment thereof; coupled to a gene. The invention further provides a vector including the nucleic acid molecule and cells harboring the nucleic acid molecule.

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