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1. 发明专利

JPH10271990A NEW MICROORGANISM NEUTRALIZING STRONGLY ACIDIC SOIL 失效
公开(公告)号：JPH10271990A
公开(公告)日：1998-10-13
申请号：JP9669897
申请日：1997-03-31
申请人： KANAGAWA PREFECTURE , SUMITOMO CONST
发明人： SOUDA IKUO , SADA MASAO , DOI YOICHI
IPC分类号： C12N1/20 , B09C1/10 , C02F3/34 , C09K17/32 , C09K101/00 , C12R1/15
摘要： PROBLEM TO BE SOLVED: To provide a microorganism capable of removing aluminum ion and manganese ion from a strongly acidic soil or a river water containing a high concentration of aluminum ion or manganese ion, and also neutralizing them. SOLUTION: This new microorganism capable of neutralizing a strongly acidic soil is Corynebacterium sp. SS-6994 (FERM P-16149) having resistances to an acid, aluminum and manganese, capable of growing under and environment containing the strongly acidic aluminum ion and manganese ion, producing ammonium ion, and taking up the aluminum ion or manganese ion or producing an extracellular protein capable of absorbing the aluminum or manganese ion.

2. 发明专利

JPH0965875A NEW BACTERIUM DECOMPOSING PCBS 失效
公开(公告)号：JPH0965875A
公开(公告)日：1997-03-11
申请号：JP24516795
申请日：1995-08-31
申请人： KANAGAWA PREFECTURE , SUMIKON SERUTETSUKU KK
发明人： SOUDA IKUO , HAYASHI MICHIO
IPC分类号： C12N1/20 , A62D3/00 , A62D3/02 , B09C1/10 , C02F3/34 , C12R1/40
摘要： PROBLEM TO BE SOLVED: To obtain a new bacterium having resistance to organic solvents, excellent in ability to decompose poly-chlorinated biphenyls(PCBs), capable of decomposing PCBs in a short time and having also excellent ability to decompose a slightly decomposable coplanar PCBs having strong toxicity. SOLUTION: This new bacterium is Pseudomonas putida SN-4992 (FERM- P-15072) having resistance to organic solvents, excellent in decomposing property of poly-chlorinated biphenyls (PCBs), capable of decomposing in a short time and having high decomposing ability to PCBs having high concentration (about 1,000ppm) and having also excellent ability to decompose slightly decomposable coplanar PCBs having strong toxicity. The new bacterium is obtained by culturing bacteria separated from soil collected in Kanagawa prefecture in a culture medium containing biphenyl, screening a bacterium decomposing biphenyl, culturing the bacterium in a culture medium, selecting a strain having decomposing ability of PCBs and further selecting bacterial strain growing in toluene.

3. 发明专利

JPH03151890A NEW MICROORGANISM AND PRODUCTION OF 2-THIOPHENEACETIC ACID USING THE SAME 失效
公开(公告)号：JPH03151890A
公开(公告)日：1991-06-28
申请号：JP28880889
申请日：1989-11-08
申请人： KANAGAWA PREFECTURE
发明人： SOUDA IKUO , SATONAKA HAJIME , KANEKO SEIICHI , SATO YOSHIHIRO , YUGAWA HARUO , HIROI TETSUYA
IPC分类号： C12P17/00 , C12N1/20 , C12R1/39
摘要： PURPOSE:To enable mass production of the subject high-purity compound in good yield by reacting a specific microorganism with a 2-n-alkylthiophene. CONSTITUTION:A bacterium Pseudomonas sp. EK-9891 (FERM P-11097) having the following mycological properties is obtained by separation thereof from soil in KANAGAWA Prefecture with an inorganic culture medium, etc., Shape; bacillus with motility. Capable of hydrolyzing urea without hydrolyzing starch, utilizing glucose without utilizing maltose, etc., An aqueous solution containing an inorganic salt such as an Mg salt and an nitrogen source such as urea is sterilized and a 2-n-alkylthiophene expressed by the formula (m is 2-12) is then added thereto. The aforementioned bacterium is further inoculated to carry out culture at 15-35 deg.C and pH 6-8 for 7-10 days according to spinner culture with aeration by a hermetic sealing method, etc., to afford a culture, which is subsequently acidified with a mineral acid and centrifuged to remove insoluble substances. The obtained culture is then extracted with ethyl ether, etc., to recover 2-thiopheneacetic acid.

4. 发明专利

JPH07213279A MICROORGANISM CAPABLE OF IMPROVING STRONG ACID SOIL AND METHOD THEREFOR 失效
公开(公告)号：JPH07213279A
公开(公告)日：1995-08-15
申请号：JP2899294
申请日：1994-01-31
申请人： KANAGAWA PREFECTURE , KENSETSU GIJUTSU KENKYUSHO KK
发明人： SOUDA IKUO , KONISHI SHIGEKI
IPC分类号： C12N1/20 , C09K17/00 , C09K101/00 , C09K109/00 , C12R1/20
摘要： PURPOSE:To provide a method for separating, culturing and proliferating a microorganism growable under a highly acidic and high-Al environment and improving the acidity of soil by applying the microorganism together with a suitable medium into the objective soil. CONSTITUTION:This new microorganism Flavobacterium sp. is capable of growing and proliferating under a strong acidic and high-Al environment. Furthermore, this method for culturing and proliferating the microorganism belonging to the genus Flavobacterium at a low pH is to use a culture medium containing a soil solution, peptone and a yeast extract. This method for raising the pH of the acid soil and reducing Al in the acid soil is to apply the microorganism belonging to the genus Flavobacterium to the soil in the method for improving the acid soil.

5. 发明专利

JPH06277045A NEW MICROORGANISM 失效
公开(公告)号：JPH06277045A
公开(公告)日：1994-10-04
申请号：JP9383993
申请日：1993-03-30
申请人： KANAGAWA PREFECTURE
发明人： SOUDA IKUO , AWAJI NORIO
IPC分类号： C02F3/34 , C12N1/20 , C12R1/40
摘要： PURPOSE:To provide a new microorganism belonging to the genus Pseudomonas, having organic solvent resistance and an ability to decompose toluene of a prescribed concentration in a salt-containing solution, and suitable for treating sewerages contaminated with salts, organic solvents, etc. CONSTITUTION:This new microorganism is a strain, Pseudomonas putida SH-2992 (FERM-P-13564), belonging to the genus Pseudomonas, having organic solvent resistance against organic solvents containing at least one kind of benzene, toluene, and xylene, and having an ability to decompose 75% (v/v) toluene in a solution containing salts. The microorganism has following mycological properties. Morphological properties: bacillus; spore size: 0.8-1.2mum wide and 1.2-4.0mum long; growth state in a medium: optimal growth temperature range of 20-30 deg.C; physiological properties: gram reaction is negative, mobility is +, growth in an aerobic state is +, catalase reaction is +, the oxidation of ethanol into acetic acid is -.

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