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    • 6. 发明公开
    • 재조합 대장균을 이용한 라이코펜 생산 방법
    • 使用重组蛋白酶生产柠檬酸的方法
    • KR1020080098246A
    • 2008-11-07
    • KR1020070043682
    • 2007-05-04
    • 아미코젠주식회사
    • 조남륜박민수이동현정호승김종근
    • C12N1/21
    • C12P23/00C12N9/0004C12N9/0083C12N9/1085C12N15/52
    • A method of manufacturing lycopene is provided to obtain an improved productivity using a recombinant E. coli and to show an antioxidant activity and an anticancer effect. A method of manufacturing lycopene comprises steps of: manufacturing a recombinant vector which is a gene selected from a group consisting of a crtE having a base sequence of a sequence number 1, a crtB having a base sequence of a sequence number 3 and a crtI having a base sequence of a sequence number 5, wherein the crtE, the crtB, and the crtI are lycopene biosynthesis associated genes; introducing the recombinant vector in the Escherichia coli; and cultivating the transduced E.coli transformant and collecting the lycopene from the culture fluid.
    • 提供番茄红素的制造方法,以使用重组大肠杆菌获得提高的生产率并显示抗氧化活性和抗癌作用。 制备番茄红素的方法包括以下步骤:制备重组载体,所述重组载体是选自具有序列号1的碱基序列的crtE,序列号3的碱基序列的crtB和具有序列号3的碱基序列的crtI的基因 序列号5的碱基序列,其中crtE,crtB和crtI是番茄红素生物合成相关基因; 将重组载体导入大肠杆菌; 培养转化的大肠杆菌转化体并从培养液中收集番茄红素。