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91. 发明授权

US09034658B2 Microfluidic devices for the capture of biological sample components 有权
标题翻译：用于捕获生物样品组分的微流体装置
公开(公告)号：US09034658B2
公开(公告)日：2015-05-19
申请号：US13511536
申请日：2010-11-23
申请人： Thomas A. Barber , Ajay Shah , John Walsh , Mehmet Toner , Ravi Kapur , Shannon L. Stott
发明人： Thomas A. Barber , Ajay Shah , John Walsh , Mehmet Toner , Ravi Kapur , Shannon L. Stott
IPC分类号： G01N33/543 , G01N33/574 , B01L3/00 , G01N33/537 , G01N33/569 , G01N15/14
CPC分类号： G01N33/57492 , B01L3/502753 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , G01N15/1484 , G01N33/537 , G01N33/54306 , G01N33/56972
摘要： Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g., in a microfluidic channel; adding to the first components of the sample a plurality of binding agents under conditions that enable a plurality of the binding agents to be linked to the analyte binding moieties to form multivalent tagging agents bound to the analyte; passing the first components of the sample past a surface to which is attached a plurality of capture agents that selectively bind to the binding agents; and capturing the analytes by providing conditions that enable the multivalent tagging agents bound to the analytes to bind to one or more of the capture agents.
摘要翻译：公开了用于从流体样品中选择性地捕获分析物例如细胞例如循环肿瘤细胞（CTC）的方法和系统。所述方法包括使样品与选择性结合分析物的分析物结合部分接触; 任选地使用基于尺寸的分离（例如在微流体通道中）分离样品的第一组分，其包含与样品的第二组分结合至结合部分的大部分分析物; 在允许多个结合剂连接到分析物结合部分以形成与分析物结合的多价标签试剂的条件下，向样品的第一组分加入多种结合剂; 使样品的第一组分通过附着有选择性结合粘合剂的多个捕获剂的表面; 并通过提供使得能够与分析物结合的多价标签试剂与一种或多种捕获剂结合的条件来捕获分析物。

92. 发明申请

US20140186879A1 Methods for Separation, Characterization and/or Identification of Microorganisms using Spectroscopy 有权
标题翻译：使用光谱分离，表征和/或鉴定微生物的方法
公开(公告)号：US20140186879A1
公开(公告)日：2014-07-03
申请号：US14146367
申请日：2014-01-02
申请人： John Walsh , Jones Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
发明人： John Walsh , Jones Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N33/6848 , H01J49/00
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

93. 发明授权

US08647835B2 Methods for separation, characterization and/or identification of microorganisms using spectroscopy 有权
标题翻译：使用光谱法分离，表征和/或鉴定微生物的方法
公开(公告)号：US08647835B2
公开(公告)日：2014-02-11
申请号：US13117976
申请日：2011-05-27
申请人： John Walsh , Jones Hyman
发明人： John Walsh , Jones Hyman
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3563 , G01N21/3577 , G01N21/3581 , G01N21/47 , G01N21/64 , G01N21/6486 , G01N21/65 , G01N2015/1402
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The method may also be useful for the physical separation and/or enrichment of two or more different or individual microorganism species contained in a mixed test sample. The invention further provides for spectroscopic interrogation of the separated microorganism sample(s) to produce measurements of the microorganism and characterizing and/or identifying the microorganism(s) in the sample using said spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。该方法还可用于混合测试样品中包含的两种或多种不同或单独的微生物物质的物理分离和/或富集。本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

94. 发明申请

US20130309714A1 Methods for Inactiviation And Extraction of Acid-Fast Bacteria for Characterization And/Or Identification Using Mass Spectrometry 有权
标题翻译：使用质谱法鉴定和/或鉴定酸快速细菌的灭活和提取方法
公开(公告)号：US20130309714A1
公开(公告)日：2013-11-21
申请号：US13828119
申请日：2013-03-14
申请人： Jones Hyman , Parampal Deol , Elizabeth Miller , Victoria Girard , Amber Gates , Sandrine Mailler , Maud Arsac , John Walsh
发明人： Jones Hyman , Parampal Deol , Elizabeth Miller , Victoria Girard , Amber Gates , Sandrine Mailler , Maud Arsac , John Walsh
IPC分类号： G01N1/40
CPC分类号： G01N1/4077 , C12Q1/24 , G01N33/5695 , G01N33/6851 , G01N2333/35 , G01N2333/355 , G01N2560/00
摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.
摘要翻译：本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

95. 发明申请

US20130209988A1 MICROFLUIDIC DEVICES FOR THE CAPTURE OF BIOLOGICAL SAMPLE COMPONENTS 有权
公开(公告)号：US20130209988A1
公开(公告)日：2013-08-15
申请号：US13511536
申请日：2010-11-23
申请人： Thomas A. Barber , Ajay Shah , John Walsh , Mehmet Toner , Ravi Kapur , Shannon L. Stott
发明人： Thomas A. Barber , Ajay Shah , John Walsh , Mehmet Toner , Ravi Kapur , Shannon L. Stott
IPC分类号： G01N33/574 , G01N33/569
CPC分类号： G01N33/57492 , B01L3/502753 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , G01N15/1484 , G01N33/537 , G01N33/54306 , G01N33/56972
摘要： Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g., in a microfluidic channel; adding to the first components of the sample a plurality of binding agents under conditions that enable a plurality of the binding agents to be linked to the analyte binding moieties to form multivalent tagging agents bound to the analyte; passing the first components of the sample past a surface to which is attached a plurality of capture agents that selectively bind to the binding agents; and capturing the analytes by providing conditions that enable the multivalent tagging agents bound to the analytes to bind to one or more of the capture agents.

96. 发明授权

US08240042B2 Methods of maintaining turbine discs to avert critical bucket attachment dovetail cracks 有权
标题翻译：维护涡轮盘以避免关键铲斗附着的方法燕尾ail裂
公开(公告)号：US08240042B2
公开(公告)日：2012-08-14
申请号：US12463931
申请日：2009-05-11
申请人： Andrew D. Williams , William Calder , John Walsh
发明人： Andrew D. Williams , William Calder , John Walsh
IPC分类号： B23P6/00
CPC分类号： F01D5/005 , B23P6/002 , B23P6/045 , F01D5/3092 , F05D2230/80 , F05D2230/90 , Y10T29/49318
摘要： Methods of to maintaining turbine discs prone to cracks in bucket attachment dovetails are disclosed. In one embodiment, wherein a turbine disc comprises a plurality of bucket attachment dovetail regions, one or more of the bucket attachment dovetail regions comprises a cooling slot, and each cooling slot comprises edges and adjacent surfaces, a method comprises inspecting the one or more of the bucket attachment dovetail regions comprising a cooling slot to confirm that each bucket attachment dovetail region meets predetermined minimum standards. The method further comprises blending the edges of the at least one of the cooling slots. The method further comprises polishing the edges of the at least one of the cooling slots. The method further comprises peening the edges of the at least one of the cooling slots. The method further comprises coating the edges and adjacent surfaces of at least one of the cooling slots.
摘要翻译：公开了保持涡轮盘容易在铲斗装卸燕尾裂缝的方法。在一个实施例中，其中涡轮盘包括多个铲斗附接燕尾区域，一个或多个铲斗附接燕尾形区域包括冷却槽，并且每个冷却槽包括边缘和相邻的表面，一种方法包括：检查一个或多个所述铲斗附接燕尾区域包括冷却槽，以确认每个铲斗附接燕尾形区域满足预定的最小标准。该方法还包括混合至少一个冷却槽的边缘。该方法还包括抛光至少一个冷却槽的边缘。该方法还包括对这些冷却槽中的至少一个的边缘进行喷丸处理。该方法还包括涂覆至少一个冷却槽的边缘和相邻表面。

97. 发明申请

US20120078600A1 Single Well Anisotropy Inversion Using Velocity Measurements 有权
标题翻译：使用速度测量的单井各向异性反演
公开(公告)号：US20120078600A1
公开(公告)日：2012-03-29
申请号：US13237937
申请日：2011-09-21
申请人： Stephen Allan Horne , John Walsh
发明人： Stephen Allan Horne , John Walsh
IPC分类号： G06G7/48
CPC分类号： G01V1/42 , G01V1/282
摘要： At least one elastic parameter of a region of interest of a formation is modeled, and at least one velocity measurement acquired in a wellbore extending in the formation is predicted based on the modeling. The model is refined based on the predicted velocity measurement(s) and based on at least one actual measurement acquired from within a single wellbore.
摘要翻译：模拟地层感兴趣区域的至少一个弹性参数，并且基于建模预测在地层中延伸的井眼中获取的至少一个速度测量值。该模型基于预测的速度测量并基于从单个井筒内获得的至少一个实际测量来精炼。

98. 发明授权

US07946248B2 Knock-down bird feeder with interlocking port 有权
标题翻译：具有互锁端口的敲门鸟饲养机
公开(公告)号：US07946248B2
公开(公告)日：2011-05-24
申请号：US12648455
申请日：2009-12-29
申请人： Scott A. Colwell , John Walsh
发明人： Scott A. Colwell , John Walsh
IPC分类号： A01K39/01
CPC分类号： A01K39/012
摘要： An elongated, tubular bird feeder comprising one or more pairs of interlocking feeder ports, where the feeder ports are of identical constructions, such that each feeder port mates with an identical feeder port. Each port defines an external perch and an external shield that is curved to fit the contour of the tube, a neck which fits tightly into an opening in the tube, and a reduced diameter portion that defines an end face, from which projects two interlocking features: a horizontally-oriented blade member; and a vertically-oriented generally sector-shaped member. When two ports are pushed together into diametrically-opposed side openings in the tube, with end faces located proximate one another, the two sector members will be located adjacent one another to define an essentially semi-circular upper region, and the two blades will be located next to one another to define an essentially horizontal blade member.
摘要翻译：一种细长的管状鸟饲料器，其包括一对或多对互锁进料器端口，其中进料器端口具有相同的结构，使得每个进料口与相同的进料口配合。每个端口限定外部鲈鱼和外部屏蔽，其弯曲以适应管的轮廓，颈部紧密地配合到管中的开口中，以及直径减小的部分，其限定端面，突出两个互锁特征：水平取向的叶片构件; 和垂直取向的大致扇形的构件。当两个端口一起推入管中的直径相对的侧开口时，端面彼此靠近，两个扇形构件将彼此相邻地定位，以限定基本上半圆形的上部区域，并且两个叶片将为彼此相邻以限定基本上水平的叶片构件。

99. 发明申请

US20110033847A1 Methods for the characterization of microorganisms on solid or semi-solid media 有权
标题翻译：在固体或半固体培养基上表征微生物的方法
公开(公告)号：US20110033847A1
公开(公告)日：2011-02-10
申请号：US12653527
申请日：2009-12-15
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe
IPC分类号： C12Q1/04 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/04
摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.
摘要翻译：本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

100. 发明申请

US20100294008A1 MOTOR DRIVE MECHANISM FOR AN ELECTRONIC DEADBOLT LOCK 有权
标题翻译：用于电子脱扣锁的电机驱动机构
公开(公告)号：US20100294008A1
公开(公告)日：2010-11-25
申请号：US12740841
申请日：2008-10-31
申请人： Victor Bogdanov , Alfred Simon Levesque , John Walsh
发明人： Victor Bogdanov , Alfred Simon Levesque , John Walsh
IPC分类号： E05B47/06 , H02K7/14 , E05B15/10 , E05B9/02
CPC分类号： E05B63/0017 , E05B47/0012 , E05B47/0692 , E05B2047/0016 , E05B2047/0023 , E05B2047/0026 , E05B2047/0031 , E05B2047/0086 , Y10T70/7062 , Y10T70/7113
摘要： An apparatus including a motor that has a rotatable output shaft extending outward from the motor and defining a rotational axis. The apparatus also includes a coupler that is coupled to the output shaft for rotation therewith, and a coil spring that is coupled to the coupler without external attachment means such that rotation of the coupler is transferred to the coil spring.
摘要翻译：一种包括马达的装置，其具有从马达向外延伸并且限定旋转轴线的可旋转输出轴。该装置还包括耦合到耦合到输出轴以与之一起旋转的耦合器，以及耦合到耦合器而没有外部附接装置的螺旋弹簧，使得耦合器的旋转传递到螺旋弹簧。

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