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1. 发明申请

US20140335558A1 Method for the Characterization of Microorganisms on Solid or Semi-Solid Media 有权
标题翻译：固体或半固体培养基上微生物表征的方法
公开(公告)号：US20140335558A1
公开(公告)日：2014-11-13
申请号：US14444708
申请日：2014-07-28
申请人： Jones M. Hyman , John D. Walsh , Thurman C. Thorpe
发明人： Jones M. Hyman , John D. Walsh , Thurman C. Thorpe
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.
摘要翻译：本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

2. 发明授权

US08735091B2 Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry 有权
标题翻译：灭活和提取用于表征和/或鉴定使用质谱法的耐酸细菌的方法
公开(公告)号：US08735091B2
公开(公告)日：2014-05-27
申请号：US13828119
申请日：2013-03-14
申请人： Jones M. Hyman , Parampal Deol , Elizabeth Miller , Victoria Girard , Amber Gates , Sandrine Mailler , Maud Arsac , John Walsh
发明人： Jones M. Hyman , Parampal Deol , Elizabeth Miller , Victoria Girard , Amber Gates , Sandrine Mailler , Maud Arsac , John Walsh
IPC分类号： C12Q1/24
CPC分类号： G01N1/4077 , C12Q1/24 , G01N33/5695 , G01N33/6851 , G01N2333/35 , G01N2333/355 , G01N2560/00
摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.
摘要翻译：本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

3. 发明授权

US07429464B2 Methods for detecting bacterial pathogens 有权
标题翻译：检测细菌病原体的方法
公开(公告)号：US07429464B2
公开(公告)日：2008-09-30
申请号：US11006025
申请日：2004-12-07
申请人： John D. Walsh , Jones M. Hyman
发明人： John D. Walsh , Jones M. Hyman
IPC分类号： C12Q1/14 , C12Q1/04
CPC分类号： G01N33/56916 , C12Q1/04 , C12Q1/10 , C12Q1/14 , G01N33/56938
摘要： The present invention provides for methods for detecting bacterial pathogens in a sample. A preferred method includes the steps of: suspending a sample comprising a medium and microorganisms, the microorganisms suspected of comprising bacterial pathogens; mixing the sample, an adsorbent and a first solution in a vessel; separating the adsorbent and the microorganisms from the medium and the bulk of the first solution and removing the medium and the bulk of the first solution from the vessel; adding a second solution to the vessel to resuspend the adsorbent and the microorganisms; and detecting agglutination of the adsorbent wherein the agglutination of the adsorbent signifies bacterial pathogens are present in the sample.
摘要翻译：本发明提供了检测样品中细菌病原体的方法。优选的方法包括以下步骤：将包含培养基和微生物的样品悬浮在怀疑包含细菌病原体的微生物中; 将样品，吸附剂和第一溶液混合在容器中; 从介质和大部分第一溶液中分离吸附剂和微生物，并从容器中除去介质和第一溶液的体积; 向容器中加入第二溶液以重新悬浮吸附剂和微生物; 并检测吸附剂的凝集，其中吸附剂的凝集表示细菌病原体存在于样品中。

4. 发明授权

US5350697A Scattered light detection apparatus 失效
标题翻译：分散光检测装置
公开(公告)号：US5350697A
公开(公告)日：1994-09-27
申请号：US574184
申请日：1990-08-28
申请人： C. Hermas Swope , John G. Link , Jones M. Hyman
发明人： C. Hermas Swope , John G. Link , Jones M. Hyman
IPC分类号： G01N21/47 , G01N33/543 , G01N33/552
CPC分类号： G01N21/47 , G01N33/54373 , G01N2021/4707 , G01N2021/4709 , Y10S435/808 , Y10S436/805
摘要： An apparatus in which scattered light is measured, said apparatus having a light source aligned to direct illumination toward an interface between a sample container and an aqueous solution at an angle less than the critical angle. A detector to measure the scattered light is located at a place outside the envelope of the critical angle.
摘要翻译：一种测量散射光的装置，所述装置具有光源，该光源被排列成以小于临界角的角度朝向样品容器和水溶液之间的界面引导照射。用于测量散射光的检测器位于临界角的包络外的位置。

5. 发明授权

US09574219B2 Device for sampling a specimen container 有权
标题翻译：用于取样样品容器的装置
公开(公告)号：US09574219B2
公开(公告)日：2017-02-21
申请号：US12800392
申请日：2010-05-14
申请人： Christopher S. Ronsick , Mark S. Wilson , Ronnie J. Robinson , John Walsh , Jones M. Hyman
发明人： Christopher S. Ronsick , Mark S. Wilson , Ronnie J. Robinson , John Walsh , Jones M. Hyman
IPC分类号： G01N1/12 , C12Q1/24 , G01N35/00 , G01N35/10
CPC分类号： C12Q1/24 , G01N35/0099 , G01N35/1079 , Y10S901/30
摘要： A sampling device for a specimen container having a closure sealing the interior of the specimen container from the environment includes a needle, a body coupled to the needle and defining a chamber in fluid communication with the needle, the body further comprising a port forming an opening in the body, the port in fluid communication with the chamber. A multitude of the sampling devices may be stored in a cassette. The sampling devices may include a second chamber for separation and concentration of a microbial agent present in a sample. In this configuration second chamber is connected to the first chamber via a valve or the like.
摘要翻译：用于样本容器的采样装置具有将样本容器的内部与环境密封的封闭件，其包括：针头，与所述针头相连并且限定与所述针流体连通的室，所述主体还包括形成开口在身体中，与腔室流体连通的端口。多个采样装置可以存储在盒中。采样装置可以包括用于分离和浓缩样品中存在的微生物剂的第二室。在该构造中，第二室通过阀等连接到第一室。

6. 发明授权

US08666673B2 Identification and/or characterization of a microbial agent using taxonomic hierarchical classification 有权
标题翻译：使用分类层次分类鉴定和/或表征微生物
公开(公告)号：US08666673B2
公开(公告)日：2014-03-04
申请号：US12800379
申请日：2010-05-14
申请人： Michael Ullery , Erin Mathias , Jones M. Hyman , John D. Walsh
发明人： Michael Ullery , Erin Mathias , Jones M. Hyman , John D. Walsh
IPC分类号： G01N33/50
CPC分类号： C12Q1/04 , G01N21/6486 , G06F19/24
摘要： A method for identification and/or characterization of a microbial agent present in a sample includes a step of analytical test data (e.g., obtaining intrinsic fluorescence values over a range of emission wavelengths) from the microbial agent. The analytical test data is transformed thereby minimizing strain to strain variations within an organism group. With the aid of a programmed computer, a multi-level classification algorithm coded as a set of processing instructions operates on the transformed analytic test data. The multiple levels correspond to different levels in a taxonomic hierarchy for microbial agents suspected of being in the sample.
摘要翻译：用于鉴定和/或表征样品中存在的微生物剂的方法包括从微生物剂中分析测试数据（例如，在发射波长范围内获得固有荧光值）的步骤。转化分析试验数据，从而使生物体组内的应变变化最小化。借助编程的计算机，编码为一组处理指令的多级分类算法对转换的分析测试数据进行操作。多个级别对应于涉嫌在样品中的微生物剂的分类层级中的不同水平。

7. 发明申请

US20100291619A1 Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample 有权
标题翻译：用于培养样品容器的组合检测仪器和用于鉴定和/或表征样品中的微生物剂的仪器
公开(公告)号：US20100291619A1
公开(公告)日：2010-11-18
申请号：US12800467
申请日：2010-05-14
申请人： Ronnie J. Robinson , Mark S. Wilson , Christopher S. Ronsick , John D. Walsh , Jones M. Hyman , Thurman Thorpe , Bradford G. Clay
发明人： Ronnie J. Robinson , Mark S. Wilson , Christopher S. Ronsick , John D. Walsh , Jones M. Hyman , Thurman Thorpe , Bradford G. Clay
IPC分类号： C12Q1/04 , C12M1/34
CPC分类号： C12Q1/04 , C12M41/36 , C12Q1/02 , G01N35/00603 , G01N35/0099 , G01N35/026 , G01N2035/00148 , G01N2035/0406 , G01N2035/0453 , G01N2035/0465
摘要： A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.
摘要翻译：检测仪器确定样品容器（例如血培养瓶）是否对其中存在微生物剂生长是阳性的。当容器被认为是正的时，它被提供（例如，转移或暴露于）到执行微生物剂的鉴定和/或表征的自动化仪器。识别和/或表征仪器将样品的一部分从样品容器中取出并将其放入一次性分离和浓缩装置中。微生物剂通过选择性裂解可能存在的和非离子的非微生物剂细胞材料而浓缩。阅读模块使用光谱方法读取浓缩的微生物剂，例如固有荧光的测量。当微生物剂保持集中在一次性装置中时，可能发生这种询问。

8. 发明申请

US20100285447A1 Methods for Antimicrobial Resistance Determination 有权
标题翻译：抗菌素耐药性测定方法
公开(公告)号：US20100285447A1
公开(公告)日：2010-11-11
申请号：US12774947
申请日：2010-05-06
申请人： John Walsh , Jones M. Hyman
发明人： John Walsh , Jones M. Hyman
IPC分类号： C12Q1/18 , C12Q1/68 , G01N33/53 , C12Q1/70 , C12M1/34
CPC分类号： C12Q1/04 , C12Q1/025 , G01N33/56911
摘要： The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system.
摘要翻译：本发明涉及用于测定微生物抗生素抗性状态的方法和系统。本发明还提供了在单个系统内测定原位微生物的抗生素抗性状态的方法。

9. 发明申请

US20100129814A1 Method for separation and characterization of microorganisms using identifier agents 有权
标题翻译：使用识别剂分离和表征微生物的方法
公开(公告)号：US20100129814A1
公开(公告)日：2010-05-27
申请号：US12589985
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
IPC分类号： C12Q1/68 , G01N33/567 , C12Q1/37 , C12Q1/04
CPC分类号： C12Q1/24 , C12Q1/04 , C12Q1/37 , C12Q1/6816 , G01N21/35 , G01N21/3581 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果的识别剂或微生物样品中识别剂的代谢形式。

10. 发明授权

US07183073B2 Method for detecting microorganisms 有权
标题翻译：微生物检测方法
公开(公告)号：US07183073B2
公开(公告)日：2007-02-27
申请号：US10428655
申请日：2003-05-02
申请人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
发明人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
IPC分类号： C12Q1/02 , C12Q1/04 , C12N11/02 , C12N11/10 , C12N11/08 , C12M1/34
CPC分类号： C12M25/14 , C12Q1/04 , C12Q1/045 , C12Q1/24
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganisms growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译：提供了用于从大量流体样品中分离和培养微生物的装置和方法。该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。培养后，微生物菌落易于在层的表面进行收获和测试。固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。相邻的支撑层可以在固定层的上方和/或下方。固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。膜可以嵌入在固定层中以增强微生物的可见性并促进微生物收获。

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