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21. 发明申请

US20120073016A1 AXMI-150 DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-150 DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20120073016A1
公开(公告)日：2012-03-22
申请号：US13302428
申请日：2011-11-22
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： A01H1/06 , C12N15/82 , A01H5/00 , C12N1/21 , C12N5/10 , C12N15/32 , C12N15/63
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，SEQ ID NO：1，3，4，5，6，7， 8，9，10，11或12，以及其变体和片段。

22. 发明申请

US20100197592A1 VARIANT AXMI-R1 DELTA ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译：变体AXMI-R1 DELTA内毒素基因及其使用方法
公开(公告)号：US20100197592A1
公开(公告)日：2010-08-05
申请号：US12701058
申请日：2010-02-05
申请人： Volker Heinrichs
发明人： Volker Heinrichs
IPC分类号： C07K14/325 , A01N37/44 , A01P7/04
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, nucleic acid molecules encoding variant AXMI-R1 sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码变体AXMI-R1序列的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。

23. 发明申请

US20090205076A1 DIRECTED EVOLUTION OF GRG31 AND GRG36 EPSP SYNTHASE ENZYMES 有权
标题翻译： GRG31和GRG36 EPSP合成酶的指导进化
公开(公告)号：US20090205076A1
公开(公告)日：2009-08-13
申请号：US12362774
申请日：2009-01-30
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： A01H1/00 , C07H21/00 , C12N15/63 , C12N1/21 , C12N5/04 , A01H5/00 , C12N9/00 , C12P21/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

24. 发明授权

US08247535B2 Directed evolution of GRG31 EPSP synthase enzyme 有权
标题翻译： GRG31 EPSP合成酶的定向进化
公开(公告)号：US08247535B2
公开(公告)日：2012-08-21
申请号：US13101351
申请日：2011-05-05
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/88 , A61K38/43 , A61K380/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

25. 发明授权

US07960616B2 Directed evolution of GRG31 EPSP synthase enzyme 有权
标题翻译： GRG31 EPSP合成酶的定向进化
公开(公告)号：US07960616B2
公开(公告)日：2011-06-14
申请号：US12362774
申请日：2009-01-30
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

26. 发明申请

US20100004176A1 AXMI-115, AXMI-113, AXMI-005, AXMI-163 AND AXMI-184: INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 有权
标题翻译： AXMI-115，AXMI-113，AXMI-005，AXMI-163和AXMI-184：杀虫蛋白及其使用方法
公开(公告)号：US20100004176A1
公开(公告)日：2010-01-07
申请号：US12497221
申请日：2009-07-02
申请人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
IPC分类号： A61K38/16 , C07H21/04 , C12N15/63 , C12N5/00 , C07K14/00 , C07K16/00
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予宿主细胞杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。组合物还包含转化的宿主细胞。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。

27. 发明申请

US20090144852A1 AXMI-066 AND AXMI-076: DELTA-ENDOTOXIN PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-066和AXMI-076：DELTA-内毒素蛋白及其使用方法
公开(公告)号：US20090144852A1
公开(公告)日：2009-06-04
申请号：US12252453
申请日：2008-10-16
申请人： Daniel J. Tomso , Nalini Desai , Shruti Agarwal , Kimberly S. Sampson , Sandra Volrath , Volker Heinrichs
发明人： Daniel J. Tomso , Nalini Desai , Shruti Agarwal , Kimberly S. Sampson , Sandra Volrath , Volker Heinrichs
IPC分类号： C12N15/82 , A01H5/00 , C12N15/11 , C12N15/00 , C12N1/21 , C12N5/04 , C07K14/00 , A61K38/16 , C12P21/02
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5, 2, or 10, the nucleotide sequence set forth in SEQ ID NO:4, 1, 3, 4, 6, 9, or 11, or the nucleotide sequence deposited in a bacterial host as Accession No. B-50045, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：5,2或10所示氨基酸序列的核苷酸序列，SEQ ID NO：4,1,3,4 ，6,9或11个核苷酸序列，或以细菌宿主保藏号B-50045以及其变体和片段沉积的核苷酸序列。

28. 发明申请

US20070295251A1 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译： EPSP合成：组合物和使用方法
公开(公告)号：US20070295251A1
公开(公告)日：2007-12-27
申请号：US11762526
申请日：2007-06-13
申请人： Volker Heinrichs
发明人： Volker Heinrichs
IPC分类号： A01G23/02 , A01H1/00 , A01H5/00 , C07H21/02 , C07H21/04 , C12N5/04
CPC分类号： C12N9/1092 , C12N15/8275
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors.
摘要翻译：提供了在细菌，植物，植物细胞，组织和种子中赋予草甘膦耐受性的组合物和方法。组合物包括新型EPSP合成酶和编码这种酶的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。

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