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1. 发明申请

US20140298538A1 AXMI205 VARIANT PROTEINS AND METHODS OF USE 有权
标题翻译： AXMI205变种蛋白及其使用方法
公开(公告)号：US20140298538A1
公开(公告)日：2014-10-02
申请号：US14233426
申请日：2012-07-27
申请人： Volker Heinrichs , Jayme Williams
发明人： Volker Heinrichs , Jayme Williams
IPC分类号： C12N15/82 , C07K16/12 , C07K14/22 , A01N37/44
CPC分类号： C12N15/8279 , A01N37/46 , A01N63/02 , A01N65/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7, 8, 9, 10, 11, or 12, the nucleotide sequence set forth in SEQ ID NO:4, 5, or 6, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：7,8,9,10,11或12所示氨基酸序列的核酸分子的核酸分子，SEQ ID NO：4所示的核苷酸序列， 5或6，以及其变体和片段。

2. 发明申请

US20110236950A1 GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 有权
标题翻译： GRG23 EPSP合成：组合物和使用方法
公开(公告)号：US20110236950A1
公开(公告)日：2011-09-29
申请号：US12856082
申请日：2010-08-13
申请人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg , Volker Heinrichs
发明人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

3. 发明申请

US20100160231A1 AXMI-150 DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-150 DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20100160231A1
公开(公告)日：2010-06-24
申请号：US12646004
申请日：2009-12-23
申请人： KIMBERLY S. SAMPSON , DANIEL J. TOMSO , VOLKER HEINRICHS
发明人： KIMBERLY S. SAMPSON , DANIEL J. TOMSO , VOLKER HEINRICHS
IPC分类号： A01N33/00 , C07K14/00 , C07K7/06 , C07K7/08 , A01P15/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。具体而言，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，即SEQ ID NO：1,3,4,5,6,7， 8，9，10，11或12，以及其变体和片段。

4. 发明授权

US06406910B1 Recombination of insertion modified nucleic acids 失效
公开(公告)号：US06406910B1
公开(公告)日：2002-06-18
申请号：US09723424
申请日：2000-11-27
申请人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
发明人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C12N15/8274 , C12N15/10 , C12N15/1027 , C12N15/8216 , C12N15/8289 , Y10T436/143333
摘要： Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.

5. 发明授权

US08575425B2 AXMI-205 pesticidal gene and methods for its use 有权
标题翻译： AXMI-205杀虫基因及其使用方法
公开(公告)号：US08575425B2
公开(公告)日：2013-11-05
申请号：US12828594
申请日：2010-07-01
申请人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo , Volker Heinrichs
发明人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo , Volker Heinrichs
IPC分类号： A01H5/00 , C07K14/22 , A01N37/18 , C12N15/31 , C12N15/63 , C12N15/82 , C12N1/21
CPC分类号： C07K14/195 , C07K14/22 , C07K16/1203 , C12N15/8285 , C12N15/8286 , C12Q1/6876 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列，以及其变体和片段。

6. 发明申请

US20130097728A1 AXMI115 VARIANT INSECTICIDAL GENE AND METHODS FOR ITS USE 审中-公开
标题翻译： AXMI115变种杀虫剂基因及其使用方法
公开(公告)号：US20130097728A1
公开(公告)日：2013-04-18
申请号：US13439269
申请日：2012-04-04
申请人： Volker Heinrichs , Duane Lehtinen
发明人： Volker Heinrichs , Duane Lehtinen
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N37/44 , A01N63/02 , C07K14/32 , C07K14/325 , C07K2319/00 , C07K2319/24 , C12N15/8279 , C12N15/8285 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。毒素编码序列可用于DNA构建体或表达盒中用于在植物和细菌中表达。组合物包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了多核苷酸序列和由此编码的毒素蛋白质。还提供了与这些氨基酸序列特异性结合的抗体。特别地，本发明包括编码融合蛋白的核苷酸序列，以及其生物活性变体及其片段，其中融合蛋白含有SEQ ID NO：43的C-末端部分。融合蛋白还可含有SEQ ID NO：45的N-末端部分。本发明还包括SEQ ID NO：47和1-14的核苷酸序列，或编码SEQ ID NO：48和15-31所示氨基酸序列的核苷酸序列，包括生物活性变体和片段。

7. 发明授权

US08283523B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08283523B2
公开(公告)日：2012-10-09
申请号：US12856072
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。

8. 发明授权

US08252981B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252981B2
公开(公告)日：2012-08-28
申请号：US12856058
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 33, or the nucleotide sequence set forth in SEQ ID NO: 32.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：33所示的氨基酸序列的核苷酸序列或SEQ ID NO：32所示的核苷酸序列的分离的多核苷酸。

9. 发明授权

US08084416B2 AXMI-150 delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-150δ-内毒素基因及其使用方法
公开(公告)号：US08084416B2
公开(公告)日：2011-12-27
申请号：US12646004
申请日：2009-12-23
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： C07K14/00 , A01N33/00 , A01P15/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。具体而言，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，即SEQ ID NO：1,3,4,5,6,7， 8，9，10，11或12，以及其变体和片段。

10. 发明申请

US20110223648A1 DIRECTED EVOLUTION OF GRG31 EPSP SYNTHASE ENZYME 有权
标题翻译： GRG31 EPSP合成酶的指导进化
公开(公告)号：US20110223648A1
公开(公告)日：2011-09-15
申请号：US13101351
申请日：2011-05-05
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N9/10
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

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