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31. 发明申请

US20130117884A1 AXMI-001, AXMI-002, AXMI-030, AXMI-035, AND AXMI-045: TOXIN GENES AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-001，AXMI-002，AXMI-030，AXMI-035和AXMI-045：毒素基因及其使用方法
公开(公告)号：US20130117884A1
公开(公告)日：2013-05-09
申请号：US13732467
申请日：2013-01-02
申请人： ATHENIX CORP.
发明人： Tracy Hargiss , Rebekah Deter , Cheryl L Peters , Sandra Volrath , Daniel J Tomso
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:6-11, or the nucleotide sequence set forth in SEQ ID NO:1-5, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：6-11所示氨基酸序列的核苷酸序列或SEQ ID NO：1-5所示的核苷酸序列，以及变体和片段。

32. 发明授权

US08314292B2 AXMI-028 and AXMI-029, a family of novel delta-endotoxin genes and methods for their use 有权
标题翻译： AXMI-028和AXMI-029是一种新型的δ-内毒素基因及其使用方法
公开(公告)号：US08314292B2
公开(公告)日：2012-11-20
申请号：US12166124
申请日：2008-07-01
申请人： Nadine Carozzi , Michael G. Koziel , Tracy Hargiss , Nicholas B. Duck
发明人： Nadine Carozzi , Michael G. Koziel , Tracy Hargiss , Nicholas B. Duck
IPC分类号： C12N15/82
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。

33. 发明授权

US08283523B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08283523B2
公开(公告)日：2012-10-09
申请号：US12856072
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。

34. 发明授权

US08252981B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252981B2
公开(公告)日：2012-08-28
申请号：US12856058
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 33, or the nucleotide sequence set forth in SEQ ID NO: 32.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：33所示的氨基酸序列的核苷酸序列或SEQ ID NO：32所示的核苷酸序列的分离的多核苷酸。

35. 发明授权

US08236757B2 AXMI-027, AXMI-036 and AXMI-038, a family of delta-endotoxin genes and methods for their use 有权
标题翻译： AXMI-027，AXMI-036和AXMI-038，一种δ-内毒素基因及其使用方法
公开(公告)号：US08236757B2
公开(公告)日：2012-08-07
申请号：US12691115
申请日：2010-01-21
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck
IPC分类号： A01N37/18 , C07K14/325
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,11和13所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,10和12所示的核苷酸序列，作为以及其变体和片段。

36. 发明授权

US08084416B2 AXMI-150 delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-150δ-内毒素基因及其使用方法
公开(公告)号：US08084416B2
公开(公告)日：2011-12-27
申请号：US12646004
申请日：2009-12-23
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： C07K14/00 , A01N33/00 , A01P15/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。具体而言，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，即SEQ ID NO：1,3,4,5,6,7， 8，9，10，11或12，以及其变体和片段。

37. 发明授权

US08076533B2 Methods and compositions for controlling plant pests 有权
标题翻译：防治植物害虫的方法和组成
公开(公告)号：US08076533B2
公开(公告)日：2011-12-13
申请号：US13027816
申请日：2011-02-15
申请人： Kimberly S. Sampson , Daniel John Tomso
发明人： Kimberly S. Sampson , Daniel John Tomso
IPC分类号： C12N15/82 , C12N1/20 , C12N5/00 , C12P21/06 , A01H5/00
CPC分类号： C07K14/325 , A01N37/46 , C07K14/32 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了包含编码SEQ ID NO：13-24所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12和25-44所示的核苷酸序列的分离的核酸分子，以及作为其变体和片段。

38. 发明申请

US20110203015A1 AXMI221z, AXMI222z, AXMI223z, AXMI224z, AND AXMI225z DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译： AXMI221z，AXMI222z，AXMI223z，AXMI224z和AXMI225Z DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20110203015A1
公开(公告)日：2011-08-18
申请号：US13030415
申请日：2011-02-18
申请人： Kimberly Sampson , Daniel Tomso
发明人： Kimberly Sampson , Daniel Tomso
IPC分类号： A01H5/00 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C07K14/00 , A01N37/18 , C12P21/00 , A01H1/00 , C12N15/82 , A01H5/10 , A01P7/04
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , C07K16/1278 , C12N15/8279 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-32, or the nucleotide sequence set forth in SEQ ID NO:1-20, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-32所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-20所示的核苷酸序列，以及变体和片段。

39. 发明申请

US20110203014A1 AXMI218, AXMI219, AXMI220, AXMI226, AXMI227, AXMI228, AXMI229, AXMI230, AND AXMI231 DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译： AXMI218，AXMI219，AXMI220，AXMI226，AXMI227，AXMI228，AXMI229，AXMI230和AXMI231三角内毒素基因及其使用方法
公开(公告)号：US20110203014A1
公开(公告)日：2011-08-18
申请号：US13030399
申请日：2011-02-18
申请人： Kimberly Sampson , Daniel Tomso
发明人： Kimberly Sampson , Daniel Tomso
IPC分类号： A01H5/00 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C07K14/00 , A01N37/18 , C12P21/00 , A01H1/00 , C12N15/82 , A01H5/10 , A01P7/04
CPC分类号： C07K14/325 , A01N37/46 , A01N63/02 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：13-30所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12所示的核苷酸序列，以及变体和片段。

40. 发明申请

US20110197314A1 Methods and Compositions for Controlling Plant Pests 有权
标题翻译：控制植物害虫的方法和组成
公开(公告)号：US20110197314A1
公开(公告)日：2011-08-11
申请号：US13027816
申请日：2011-02-15
申请人： Kimberly S. Sampson , Daniel John Tomso
发明人： Kimberly S. Sampson , Daniel John Tomso
IPC分类号： A01H1/00 , C07K14/32 , A01N37/18 , C12P21/00 , C12N15/31 , C12N15/63 , C12N1/21 , C12N5/04 , A01H5/00 , A01H5/10
CPC分类号： C07K14/325 , A01N37/46 , C07K14/32 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了包含编码SEQ ID NO：13-24所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12和25-44所示的核苷酸序列的分离的核酸分子，以及作为其变体和片段。

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