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    • 31. 发明申请
    • METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI
    • 用于产生复杂TRAIT LOCI的方法和组合物
    • US20130198888A1
    • 2013-08-01
    • US13748704
    • 2013-01-24
    • Pioneer Hi-Bred International, Inc.E.I. Du Pont De Nemours And Company
    • Carl FalcoMichael LassnerZhongsen LiChristopher Scelonge
    • A01H5/10A01H1/02
    • C12N15/8213C12N9/16C12N9/22
    • Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.
    • 提供组合物和方法用于将多个独立的转基因基因座堆叠到植物的基因组中。 组合物包括植物,种子或植物细胞,其包含至少一个转基因靶位点和至少一个目的基因组集成在基因组窗口内的不同基因组位点。 可以使用植物育种技术,使得转基因靶位点和感兴趣的基因组座位可以一起繁殖。 以这种方式,可以在基因组窗口内产生多个独立的转基因整合以产生复杂的性状基因座。 复杂特征位点的设计使得目的基因座和/或基因座位点彼此独立地分离,从而提供通过繁殖和遗传特异性元件改变复杂性状基因座的益处。 也可以使用各种方法来修饰靶位点,使得它们含有多种感兴趣的多核苷酸。
    • 39. 发明授权
    • Crosslinked fluoropolymer networks
    • 交联氟聚合物网络
    • US08394870B2
    • 2013-03-12
    • US12504320
    • 2009-07-16
    • Ming-Hong HungBruno AmeduriGeorgi Kostov
    • Ming-Hong HungBruno AmeduriGeorgi Kostov
    • C08F16/24
    • C08F214/184C08F8/00C08F8/02C08F8/04C08F8/12C08F8/14C08F8/20C08F214/18C08F299/00C08F214/222
    • Disclosed herein is a crosslinked fluoropolymer network formed by the free radical initiated crosslinking of a diacrylate fluoropolymer The diacrylate copolymer is of formula CH2═CR′COO—(CH2)n—R—(CH2)n—OOCR′═CH2, wherein R is selected from the group consisting of i) an oligomer comprising copolymerized units of vinylidene fluoride and perfluoro(methyl vinyl ether), ii) an oligomer comprising copolymerized units of vinylidene fluoride and hexafluoropropylene, iii) an oligomer comprising copolymerized units of tetrafluoroethylene and perfluoro(methyl vinyl ether), and iv) an oligomer comprising copolymerized units of tetrafluoroethylene and a hydrocarbon olefin, R′ is H or —CH3, n is 1-4 and wherein said oligomer has a number average molecular weight of 1000 to 25,000 daltons. The source of the free radicals may be a photoinitiator or an organic peroxide.
    • 本文公开了通过二丙烯酸酯含氟聚合物的自由基引发交联形成的交联的氟聚合物网络。二丙烯酸酯共聚物具有式CH 2 = CR'COO-(CH 2)n -R-(CH 2)n -OOCR' = CH 2,其中R为 选自i)包含偏二氟乙烯和全氟(甲基乙烯基醚)的共聚单元的低聚物,ii)包含偏二氟乙烯和六氟丙烯的共聚单元的低聚物,iii)包含四氟乙烯和全氟(甲基)丙烯的共聚单元的低聚物 乙烯基醚),和iv)包含四氟乙烯和烃烯烃的共聚单元的低聚物,R'是H或-CH 3,n是1-4,并且其中所述低聚物的数均分子量为1000-25,000道尔顿。 自由基的来源可以是光引发剂或有机过氧化物。