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    • 41. 发明授权
    • Light scatter-based immunoassay without particle self aggregation
    • 基于光散射的免疫测定,无颗粒自聚集
    • US5589401A
    • 1996-12-31
    • US286778
    • 1994-08-05
    • W. Peter HansenMichael Cennerazzo
    • W. Peter HansenMichael Cennerazzo
    • G01N15/02G01N15/14G01N33/543G01N33/553G01N33/538G01N33/544
    • G01N15/14G01N33/54313G01N33/553G01N15/0205
    • A homogeneous immunoassay method for the simultaneous determination of one or more antibody, antigen or hapten analytes in a fluid sample, that comprises the quantification of the effect of said analytes on the statistical changes in a dimension of a light scatter pulse height distribution histogram of relatively large diameter monodisperse binding molecule-coated polymeric microspheres induced by the binding to said microspheres of polydisperse binding molecule-coated colloid metal particles of relatively small diameter. For simultaneous assays of multiple analytes, different diameter or refractive index microspheres are assigned to each analyte. The assay may be used in forward binding, displacement, inhibition, and competition type systems, with the direction of the change in histogram dimension depending on the system. A convenient dimension to measure is the normalized peak width of a graphical representation of the histogram. For simultaneous assays for multiple analytes, a monodisperse polymeric microsphere of unique diameter or refractive index is dedicated to each analyte, so as to generate multiple histograms, one for each analyte. Polydisperse binding molecule-coated colloid metal particles, when used in effective concentrations, will also serve as a scavenger means to reduce the interfering effects of non-specific substances in the analyte-containing fluid samples, particularly when said samples are of biological origin.
    • 用于同时测定流体样品中一种或多种抗体,抗原或半抗原分析物的均匀免疫测定方法,其包括对所述分析物对相对较轻的光散射脉冲高度分布直方图的维度的统计变化的量化的量化 通过与所述微球体结合相对小直径的多分散结合分子包被的胶体金属颗粒诱导的大直径单分散结合分子包被的聚合物微球。 对于多个分析物的同时测定,将不同直径或折射率微球分配给每个分析物。 该测定可用于正向结合,置换,抑制和竞争类型系统,其中取决于系统的直方图尺寸的变化方向。 一个方便的测量尺寸是直方图的图形表示的归一化峰宽。 对于多个分析物的同时测定,独特直径或折射率的单分散聚合物微球专用于每个分析物,以便产生多个直方图,每个分析物一个。 当以有效浓度使用时,多分散结合分子涂覆的胶体金属颗粒也将用作清除剂手段,以减少非特异性物质在含分析物的流体样品中的干扰作用,特别是当所述样品是生物来源时。