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1. 发明申请

US20080003695A1 Enriched Pag-55 Fraction and Methods for Early Detection of Preganancy in Ungulate Animals 有权
标题翻译：丰富的Pag-55分子和早期检测有蹄类动物前体的方法
公开(公告)号：US20080003695A1
公开(公告)日：2008-01-03
申请号：US11587391
申请日：2005-04-27
申请人： Nagappan Mathialagan , Michael Mcgrath , Robert Schenkel
发明人： Nagappan Mathialagan , Michael Mcgrath , Robert Schenkel
IPC分类号： G01N33/53 , C07K14/00 , C07K16/18
CPC分类号： C07K16/18 , C07K14/4715 , G01N33/689 , G01N2800/368 , Y10S424/811
摘要： Disclosed are methods for determining the pregnancy status of an ungulate animal by detecting the level of PAG-55 protein fraction. These methods may involve the detection of PAG-55 enriched proteins in the PAG-55 protein fraction by using novel polyclonal and monoclonal antibodies that have been generated using the PAG-55 enriched protein fraction as an immulogen. The novel PAG-55 protein fraction indicates that the animal is pregnant when the acidic PAG-55 protein fraction, or antigenic components thereof, are present at an elevated level in a sample. The present invention also provides for methods of obtaining the PAG-55 enriched protein fraction. The invention provides accurate methods of detecting pregnancy at early stages and has the benefit of allowing early post partum detection of the pregnancy status with few1 false positive results. The antibodies for detecting PAG-55 proteins will also be useful in combination with detecting elevated progesterone levels in the animal, thus providing an even more effective pregnancy detection method. The benefit of this early pregnancy detection is that identifying animals that are not pregnant very shortly after breeding, allows for timely rebreeding and minimizes the amount of time the animal is open. Also disclosed are methods for making a breeding decision for an animal. These methods allow a herd manager to make breeding decisions based on the levels of PAG-55 protein fraction and optionally, progesterone levels detected in a biological sample taken from an animal suspected of being in the early stages of pregnancy.
摘要翻译：公开了通过检测PAG-55蛋白质级分的水平来确定有蹄动物的怀孕状态的方法。这些方法可以通过使用已经使用富含PAG-55的蛋白质级分作为免疫原产生的新型多克隆和单克隆抗体来检测PAG-55蛋白质级分中的PAG-55富集的蛋白质。新型PAG-55蛋白质级分表明当样品中升高的水平存在酸性PAG-55蛋白质级分或其抗原性组分时，该动物是怀孕的。本发明还提供了获得富含PAG-55的蛋白质级分的方法。本发明提供了早期检测怀孕的准确方法，并且具有允许早期产后检测怀孕状态的益处，并且几乎没有假阳性结果。用于检测PAG-55蛋白的抗体也可用于检测动物中升高的孕酮水平，从而提供更有效的妊娠检测方法。这种早期妊娠检测的好处是识别在繁殖后不久怀孕的动物，允许及时重新种植，并最大限度地减少动物开放的时间。还公开了用于为动物进行育种决定的方法。这些方法允许畜牧经理基于PAG-55蛋白质级分的水平进行育种决定，并且任选地，在从怀疑怀孕早期阶段的动物取得的生物样品中检测到孕酮水平。

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