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    • 5. 发明申请
    • Nucleic acid extraction method
    • 核酸提取法
    • US20070015177A1
    • 2007-01-18
    • US11399420
    • 2006-04-07
    • Pierre-Alain MaronDavid LejonKarine BizetEsmeralda Carvalho
    • Pierre-Alain MaronDavid LejonKarine BizetEsmeralda Carvalho
    • C12Q1/68C12N1/08C07H21/04
    • C12N15/1003
    • The invention relates to a method for the extraction of nucleic acids from microorganisms and to the use thereof in a method for the analysis of a microbial population in a given environment, e.g. a population of microorganisms taken from the air. In particular, the invention relates to an extraction method in which the following three lysis steps are performed successively in any order, namely: (i) chemical lysis of the microorganisms, consisting in bringing the microorganisms into contact with a lysis solution containing a detergent; (ii) thermal shock lysis comprising the incubation of the extract at a temperature lower than 0° C., immediately followed by the incubation of the extract at a temperature of at least 95° C. and preferably 100° C.; and mechanical lysis in which the extract is stirred in the presence of balls.
    • 本发明涉及一种从微生物中提取核酸的方法及其在给定环境例如微生物群体分析方法中的应用。 从空气中摄取的微生物群体。 具体而言,本发明涉及一种提取方法,其中以任何顺序连续进行以下三个裂解步骤,即:(i)微生物的化学裂解,使微生物与含有洗涤剂的裂解溶液接触; (ii)热休克裂解,包括在低于0℃的温度下孵育提取物,然后在至少95℃,优选100℃的温度下温育提取物。 和机械裂解,其中提取物在球存在下搅拌。