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    • 8. 发明授权
    • Stabilization of particles and reduction of sample discordance in immunoassays using casein coating of particles
    • 使用酪蛋白涂层的颗粒免疫测定中颗粒的稳定化和样品不一致性的降低
    • US06756234B2
    • 2004-06-29
    • US10029541
    • 2001-12-21
    • Wei-Chao NiDaniel W. EustaceSteve Chin-Shen Chang
    • Wei-Chao NiDaniel W. EustaceSteve Chin-Shen Chang
    • G01N33553
    • G01N33/54333G01N33/5434G01N33/54393G01N2333/4731G01N2446/84Y10S435/963Y10S436/81Y10S530/815Y10T428/2991Y10T428/2998
    • It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used for immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and few discordant samples have been developed. Casein used at a concentration of 0.05-4.0 grams per gram of paramagnetic particle (optimally approximately 0.78-1.2 grams of casein per gram of magnetic particle) has been found to confer this benefit. In addition, a process for coating solid phases has been invented, said process comprising the mixing of casein with magnetic particles at 30-60° C. for 5-180 hours, said process resulting in casein-coated paramagnetic particles which either (1) already have combined therewith active ingredients needed in the binding assay or (2) are capable of reacting with active ingredients needed in the binding assay. Furthermore, a process for using the casein-coated paramagnetic particles have been developed, said particles being directly or indirectly combined with active ingredients used in the immunoassays.
    • 已经发现酪蛋白和酪蛋白的盐可用作BSA的替代物或作为BSA的替代物,作为用于包被固相的材料,特别是用于免疫测定的磁性颗粒和用于分离所需分析物的其它结合测定。 通过使用酪蛋白,开发了具有改善的稳定性和少量不一致样品的免疫测定。 已经发现以每克顺磁性颗粒(最佳约0.78-1.2克酪蛋白/克磁性颗粒)浓度为0.05-4.0克的酪蛋白赋予这种益处。 此外,已经发明了一种用于涂布固相的方法,所述方法包括将酪蛋白与磁性颗粒在30-60℃下混合5-180小时,所述方法得到酪蛋白包被的顺磁性颗粒,其(1) 已经结合了结合测定中所需的活性成分,或(2)能够与结合测定中所需的活性成分反应。 此外,已经开发了使用酪蛋白涂覆的顺磁性颗粒的方法,所述颗粒与免疫测定中使用的活性成分直接或间接组合。