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    • 6. 发明授权
    • Method and composition for a protein transduction technology and its applications
    • 蛋白转导技术的方法和组成及其应用
    • US08722348B2
    • 2014-05-13
    • US12128320
    • 2008-05-28
    • Jianjun WangQianqian Li
    • Jianjun WangQianqian Li
    • G01N33/567
    • A61K38/00A61K47/48046A61K47/48176C12N15/88C12P21/00G01N33/5023G01N33/58G01N33/68G01N2458/15
    • A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.
    • 发明了用于将外源蛋白有效递送到哺乳动物细胞中的蛋白质转导方法,其具有靶向不同细胞区室的能力并且防止递送的蛋白质从细胞蛋白酶降解。 用于处理蛋白质的组合物在载体中具有阳离子试剂,脂质和增强剂。 该方法可以以多种方式使用,包括:使用哺乳动物细胞机制产生大量正确折叠的翻译后修饰蛋白,使用小分子荧光团进行细胞内荧光光谱和成像,并使用细胞内核磁共振光谱 活哺乳动物细胞。 该方法允许细胞生物学在原子分辨率下与生理和病理相关,并允许蛋白质治疗来治疗人类疾病。 该方法还可用于在哺乳动物细胞内递送外源蛋白质,其中外源蛋白质遵循与内源性蛋白质相似的分泌途径。