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    • 10. 发明申请
    • VITRO IMMUNIZATION
    • 体外免疫
    • US20090098090A1
    • 2009-04-16
    • US10538393
    • 2003-12-09
    • Derek Nigel John HartCameron John Turtle
    • Derek Nigel John HartCameron John Turtle
    • A61K35/14C12N5/08
    • C12N5/0636A61K2039/5158A61K2039/57C12N2501/01C12N2501/056C12N2501/23C12N2502/11
    • The present invention relates generally to a method of generating lymphocytes specific for particular antigens. More particularly, the present invention provides a method for generating antigen-reactive T- cells and even more particularly cytotoxic (CD8+) T-cells in 10 vitro specific for antigens such as peptide antigens. The method of the present invention enables in vitro T-cell priming for particular antigens such as antigens on cancer cells, pathogenic cells, viruses or cells infected with viruses. The present invention is useful in identifying particularly immunogenic antigens for immunotherapy. Furthermore, as a consequence, the present invention is useful in avoiding the need for expensive and time 15 consuming clinical trials. The present invention further provides a method for the treatment or prophylaxis of a disease or condition in a subject by generating T-cells reactive to an antigenic molecule and administering an effective amount of antigen-reactive T-cells to the subject or other compatible host. Furthermore, the present invention permits the generation of dendritic cell/T-cell populations for use in cellular immunotherapy.
    • 本发明一般涉及产生针对特定抗原特异性的淋巴细胞的方法。 更具体地,本发明提供了一种用于产生抗原反应性T细胞,甚至更具体地细胞毒性(CD8 +)T细胞的方法,其在抗原例如肽抗原中具有10种体外特异性。 本发明的方法能够在癌细胞,致病细胞,病毒或感染病毒的细胞上的特定抗原例如抗原的体外T细胞引发。 本发明可用于鉴定用于免疫治疗的特异性免疫原性抗原。 此外,因此,本发明有助于避免对昂贵和时间消耗的临床试验的需要。 本发明还提供了通过产生对抗原分子具有反应性的T细胞并向对象或其他相容宿主施用有效量的抗原反应性T细胞来治疗或预防受试者的疾病或病症的方法。 此外,本发明允许产生用于细胞免疫治疗的树突状细胞/ T细胞群体。