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1. 发明授权

US10246479B2 Method of preparation of nanopore and uses thereof 有权
公开(公告)号：US10246479B2
公开(公告)日：2019-04-02
申请号：US14391320
申请日：2013-04-08
申请人： Jingyue Ju , Shiv Kumar , Chuanjuan Tao , Minchen Chien , James J. Russo , John J. Kasianowicz , Joseph W. F. Robertson
发明人： Jingyue Ju , Shiv Kumar , Chuanjuan Tao , Minchen Chien , James J. Russo , John J. Kasianowicz , Joseph W. F. Robertson
IPC分类号： C07H19/00 , C12Q1/42 , C12Q1/48 , C12Q1/6869 , C07H19/06 , C07H19/16 , C12Q1/6883 , G01N33/487 , C12Q1/68 , B82Y5/00
摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

2. 发明申请

US20190309008A1 METHOD OF PREPARATION OF NANOPORE AND USES THEREOF 审中-公开
公开(公告)号：US20190309008A1
公开(公告)日：2019-10-10
申请号：US16371646
申请日：2019-04-01
申请人： Jingyue JU , Shiv KUMAR , Chuanjuan TAO , Minchen CHIEN , James J. RUSSO , John J. KASIANOWICZ , Joseph W.F. ROBERTSON
发明人： Jingyue JU , Shiv KUMAR , Chuanjuan TAO , Minchen CHIEN , James J. RUSSO , John J. KASIANOWICZ , Joseph W.F. ROBERTSON
IPC分类号： C07H19/00 , C12Q1/48 , C12Q1/42 , C07H19/16 , C12Q1/6869 , C07H19/06 , G01N33/487 , C12Q1/6883
摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

3. 发明申请

US20150111759A1 METHOD OF PREPARATION OF NANOPORE AND USES THEREOF 审中-公开
标题翻译：制备纳米材料的方法及其用途
公开(公告)号：US20150111759A1
公开(公告)日：2015-04-23
申请号：US14391320
申请日：2013-04-08
申请人： Jingyue Ju , Shiv Kumar , Chuanjuan Tao , Minchen Chien , James J. Russo , John J. Kasianowicz , Joseph W.F. Robertson
发明人： Jingyue Ju , Shiv Kumar , Chuanjuan Tao , Minchen Chien , James J. Russo , John J. Kasianowicz , Joseph W.F. Robertson
IPC分类号： C12Q1/68
CPC分类号： C07H19/00 , B82Y5/00 , C07H19/06 , C07H19/16 , C12Q1/42 , C12Q1/48 , C12Q1/6869 , C12Q1/6883 , C12Q2600/158 , G01N33/48721 , G01N2333/9125 , C12Q2521/525 , C12Q2521/543 , C12Q2535/107 , C12Q2565/631
摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.
摘要翻译：本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。在一些方面，本公开涉及使用标签分子对核酸进行测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

4. 发明申请

US20100122907A1 SINGLE MOLECULE MASS OR SIZE SPECTROMETRY IN SOLUTION USING A SOLITARY NANOPORE 审中-公开
标题翻译：单分子单分子质谱或尺寸分光光度计
公开(公告)号：US20100122907A1
公开(公告)日：2010-05-20
申请号：US12435667
申请日：2009-05-05
申请人： Vincent M. Stanford , John J. Kasianowicz , Joseph W.F. Robertson , Claudio G. Rodrigues , Oleg V. Krasilnikov
发明人： Vincent M. Stanford , John J. Kasianowicz , Joseph W.F. Robertson , Claudio G. Rodrigues , Oleg V. Krasilnikov
IPC分类号： G01N27/447
CPC分类号： G01N33/48721 , C12Q1/6869 , C12Q2537/165 , C12Q2563/116 , C12Q2565/631
摘要： A nanopore conductance measurement method and system is provided. The system has reservoirs of conductive fluid separated by a resistive barrier, which is perforated by a single nanometer scale pore commensurate in size with an analyte molecule in at least one of the reservoirs. The system is configured to have an ionic current driven across the reservoirs by an applied potential and the pore may be treated so that the pore surface can form associations with the analyte molecules of interest to increase the analyte molecule residence times on or in the pore. The system also comprises a means of measuring the ionic current, which current may be either direct or alternating in time, induced by an applied potential between electrodes in the conductive fluid, on each side of the barrier. The system also comprises a means of recording the ionic current time course as a time series, which includes time periods when the pore is unobstructed and also in periods when analyte molecules cause pulses of reduced conductance. The method comprises methods to delineate segments of the conductance time series into regions statistically consistent with the unobstructed pore conductance level, and pulses of reduced conductance, and also statistically stationary segments within individual pulses of reduced conductance. The method may also provide steps for interpreting the statistical analysis to yield parameters such as size, mass, and/or concentration of at least one type of analyte in solution.
摘要翻译：提供纳米孔电导测量方法和系统。该系统具有由电阻屏障分隔的导电流体的储存器，该电阻屏障通过与至少一个储存器中的分析物分子大小相当的单个纳米尺度孔穿孔。该系统被配置为具有通过施加的电势驱动跨越储存器的离子电流，并且可以处理孔以使孔表面可以与感兴趣的分析物分子形成关联，以增加分析物分子在孔上或孔中的停留时间。该系统还包括测量离子电流的手段，该电流可以是在屏障的每一侧上的导电流体中的电极之间的施加电位引起的时间上的直接或交替的电流。该系统还包括将离子电流时间过程记录为时间序列的手段，时间序列包括当孔隙畅通的时间段以及分析物分子引起电导率降低的时段。该方法包括将电导时间序列的段划分到与无阻碍的孔电导水平统计学一致的区域，以及降低的电导的脉冲以及降低的电导的单个脉冲内的统计学上固定的区段的方法。该方法还可以提供解释统计分析以产生诸如解决方案中的至少一种类型的分析物的尺寸，质量和/或浓度的参数的步骤。

5. 发明授权

US08294007B2 Method of stabilization of functional nanoscale pores for device applications 有权
标题翻译：用于器件应用的功能性纳米孔的稳定方法
公开(公告)号：US08294007B2
公开(公告)日：2012-10-23
申请号：US11070398
申请日：2005-02-28
申请人： Devanand K. Shenoy , Alok Singh , William R. Barger , John J. Kasianowicz
发明人： Devanand K. Shenoy , Alok Singh , William R. Barger , John J. Kasianowicz
IPC分类号： B05D1/28
CPC分类号： B01D61/38 , C12Q1/6869 , G01N33/48721
摘要： A membrane is disclosed made from a compound having a hydrophilic head group, an aliphatic tail group, and a polymerizable functional group. The membrane spans an aperture and may be polymerized. The membrane may be useful for DNA sequencing when the membrane includes an ion channel.
摘要翻译：公开了由具有亲水性头基，脂肪族尾基和可聚合官能团的化合物制成的膜。膜跨越孔并可以聚合。当膜包括离子通道时，膜可用于DNA测序。

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