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1. 发明申请

US20220403440A1 METHODS FOR CORRELATIVE MICROSCOPY 有权
公开(公告)号：US20220403440A1
公开(公告)日：2022-12-22
申请号：US17839586
申请日：2022-06-14
申请人： Leica Mikrosysteme GmbH
发明人： Julia KÖNIG , Paul WURZINGER
IPC分类号： C12Q1/02 , G01N21/64 , G01N23/04 , G01N1/28 , G01N1/42 , G01N23/06
摘要： A method (400) for microscopic examination of a sample (1) includes applying (410) the sample (1) to a sample holder (10) having a transparent carrier material, capturing (420) a first image (210, 220) of the sample (1) applied to the sample holder (10) using a first light-microscopy method, cryofixing, freeze-substituting, and subsequently infiltrating and embedding (430) the sample (1) together with the sample holder (10) with an embedding medium (20) in an embedding mold (90, 100), curing (440) the embedding medium (20), removing the sample (1) from the embedding mold (90, 100) together with the embedding medium (20) and the sample holder (10), capturing (450) a second image (230) of the sample (1) embedded in the cured embedding medium (20) using a second light-microscopy method, wherein at least partially identical regions of the sample (1) are captured in the first and second images, and identifying (460) at least one portion of the first image (210, 220) and one portion of the second image (230) which show identical regions of the sample (1).

2. 发明授权

US10809211B2 Modular specimen holders for high pressure freezing and X-ray crystallography of a specimen 有权
公开(公告)号：US10809211B2
公开(公告)日：2020-10-20
申请号：US16327835
申请日：2017-07-13
申请人： LEICA MIKROSYSTEME GMBH
发明人： Rainer Wogritsch , Cveta Tomova , Paul Wurzinger , Heinz Plank , Siegfried Tanki
IPC分类号： G01N23/20 , G01N23/20033 , G01N1/42 , G01N23/20025 , G01N23/20041
摘要： A modular specimen holder (10′) for high pressure freezing and/or X-ray crystallography of a specimen has a specimen holding element (100′) and an extension element (200′) connectable with and separable from each other; the specimen holding element (100′) including a tubule (120′) adapted to hold the specimen and a base element (110′) adapted to hold the tubule (120′), wherein a distance from a bottom of the base element (110′) to a top of the tubule (120′) is a first distance (d1); the extension element (200′) being connectable with the base element (110′), wherein, when the extension element (200′) and the base element (110′) are connected with each other, a second distance (d2) from a bottom of the extension element (200′) to the top of the tubule (120′) is larger than the first distance (d1).

3. 发明申请

US20040251434A1 ERGONOMIC APPARATUS FOR CUTTING SPECIMENS 审中-公开
标题翻译：用于切割样本的人体器官
公开(公告)号：US20040251434A1
公开(公告)日：2004-12-16
申请号：US10709874
申请日：2004-06-02
申请人： Leica Mikrosysteme GmbH
发明人： Michael Zimmermann , Reinhard Lihl , Andreas Hallady , Anton Lang
IPC分类号： G06M007/00
CPC分类号： G01N1/06 , G01N2001/065
摘要： In cutting devices (10), in particular microtomes or ultramicrotomes, it is necessary to preset the specimen (14) onto the knife (16). To simplify this presetting operation, which is intended to ensure the quickest and most accurate possible positioning of the knife (16) relative to the specimen (14), a stereomicroscope (12) is provided that can be adjusted in many ways using operating elements (15, 17, 19, 27). To improve the unit's ergonomics and additionally eliminate collisions during pivoting, the unit base (22) of the cutting device (10) is modified. Since the modification of the unit base must result in a lowering, this modification can be achieved by lowering the knife holder (26), the mounting surface for the linear guide being milled directly onto the unit base (22).
摘要翻译：在切割装置（10），特别是切片机或超薄切片机中，需要将样品（14）预先设置在刀具（16）上。为了简化这种预设操作，其旨在确保刀具（16）相对于试样（14）的最快和最准确的定位，提供立体显微镜（12），其可以以许多方式使用操作元件（ 15，17，19，27）。为了改善单元的人体工程学并且另外消除在枢转期间的碰撞，切割装置（10）的单元基座（22）被修改。由于单元基座的修改必须降低，所以可以通过降低刀架（26）来实现该修改，直线导轨的安装面直接被研磨到单元基座（22）上。

4. 发明申请

US20040171102A1 Apparatus and method for immunological labeling for thin tissue sections 失效
标题翻译：薄组织切片免疫标记的装置和方法
公开(公告)号：US20040171102A1
公开(公告)日：2004-09-02
申请号：US10789529
申请日：2004-02-27
申请人： Leica Mikrosysteme GmbH
发明人： Heinz Plank
IPC分类号： G01N001/30 , G01N033/48 , C12M001/34
CPC分类号： G01N35/04 , B01L9/52 , G01N1/30 , G01N1/312 , G01N33/543 , G01N33/54366 , Y10T436/13
摘要： An apparatus and a method for immunological labeling of thin tissue sections are disclosed. The treatment fluid is applied in the form of liquid droplets onto at least one slide, the arrangement of liquid droplets corresponding to the arrangement of the thin tissue sections on the carrier plate. Each slide is retained in a transport container, the transport container being embodied in the form of a trough which comprises a peripheral delimiting wall that is closed off by a base. The base of one transport containers constitutes the cover of a transport container arranged beneath it. The transport containers are stacked in a first and a second station.
摘要翻译：公开了薄组织切片的免疫标记的装置和方法。处理流体以液滴的形式施加到至少一个载玻片上，液滴的布置对应于载体板上的薄组织部分的布置。每个滑块保持在运输容器中，运输容器以槽的形式实施，该槽包括由基座封闭的外围分隔壁。一个运输容器的底部构成布置在其下方的运输容器的盖子。运输容器堆放在第一和第二站中。

5. 发明申请

US20030003577A1 Method and apparatus for preparing monolayers of cells 有权
标题翻译：用于制备细胞单层的方法和装置
公开(公告)号：US20030003577A1
公开(公告)日：2003-01-02
申请号：US10172473
申请日：2002-06-14
申请人： Leica Mikrosysteme GmbH
发明人： Heinz Horstmann
IPC分类号： C12N005/00
CPC分类号： G01N1/42 , G01N1/2813 , G01N1/36
摘要： The invention concerns an apparatus for preparing monolayers of cells. The apparatus comprises a container (6) for a cryosubstitution system and an insert (1) for the container, the insert (1) having a surface (1a) and a plurality of orifices (3). An SCS (2) together with a cellular monolayer (20) is insertable into each of the orifices (3). The SCS (2) is thus arranged perpendicular to the surface (1a) of the insert (1).
摘要翻译：本发明涉及一种制备细胞单层的装置。该装置包括用于冷冻置换系统的容器（6）和用于容器的插入件（1），插入件（1）具有表面（1a）和多个孔（3）。 SCS（2）与细胞单层（20）一起可插入每个孔（3）中。因此，SCS（2）垂直于插入件（1）的表面（1a）布置。

6. 发明申请

US20230120051A1 SAMPLE HOLDER FOR HOLDING A SAMPLE CARRIER CARRYING A SAMPLE 有权
公开(公告)号：US20230120051A1
公开(公告)日：2023-04-20
申请号：US18045179
申请日：2022-10-10
申请人： LEICA Mikrosysteme GmbH
发明人： Heinz Plank , Tim Laugks , Ulrike Laugks , Roland Thuenauer
IPC分类号： G01N1/42
摘要： A sample holder for holding a sample carrier carrying a sample includes a sample carrier fixing element. The sample carrier fixing element includes a first section configured, when in a first position, to fix the sample carrier to the sample holder, and, when in a second position, to release the sample carrier or to provide access to an area where the sample carrier is to be located. The sample carrier fixing element also includes a second section different from the first section, the second section being operable such that upon operation of the second section the first section switches from the first position into the second position or from the second position into the first position.

7. 发明申请

US20230045321A1 SYSTEM FOR LOADING AND/OR MANIPULATING A SAMPLE IN A SAMPLE TRANSFER DEVICE 有权
公开(公告)号：US20230045321A1
公开(公告)日：2023-02-09
申请号：US17873266
申请日：2022-07-26
申请人： Leica Mikrosysteme GmbH
发明人： Reinhard LIHL
IPC分类号： G01N1/28 , B01L1/02
摘要： A system (100) for loading a sample into and/or manipulating a sample in a sample transfer device (180) at cryogenic temperatures, comprising the sample transfer device (180) being configured to receive a sample through a receiving opening (182) of the sample transfer device (180) and configured to transfer the sample to a processing or analysing unit, and a dry box (110) having an interface opening (112) and being configured to be coupled to the sample transfer device (180) such that the interface opening (112) of the dry box (110) is located opposite the receiving opening (182) of the sample transfer device (180).

8. 发明申请

US20220074834A1 PREPARATION OF A SAMPLE FOR HIGH PRESSURE FREEZING 有权
公开(公告)号：US20220074834A1
公开(公告)日：2022-03-10
申请号：US17460383
申请日：2021-08-30
申请人： Leica Mikrosysteme GmbH
发明人： Rainer WOGRITSCH , Paul WURZINGER
IPC分类号： G01N1/42 , F25D3/10
摘要： The present disclosure relates to a device (10) for preparing a microscopic sample (1) for a high-pressure freezing process in which the sample (1) is provided using an arrangement comprising a middle plate (33) of a high-pressure freezing cartridge (30) and an incubation chamber (100, 200), the middle plate (33) being attached to the incubation chamber (100, 200) and being detachable from the incubation chamber (100, 200) by effecting a relative movement between the middle plate (33) and the incubation chamber (100, 200), and the sample (1) being provided on an enclosing element (37) which is fitted into an opening (36) of the middle plate (33), wherein the device (10) comprises an engagement structure (11, 21) adapted to engage with the middle plate (33) and to restrict a movement of the middle plate (33) while the incubation chamber (100, 200) is moved, such as to effect said relative movement between the middle plate (33) and the incubation chamber (100, 200) and to thereby detach the middle plate (33) from the incubation chamber (100, 200). Also, an incubation chamber (100, 200), a middle plate (33) of a high-pressure freezing cartridge (30), an arrangement comprising these components, and a corresponding method are part of the present disclosure.

9. 发明授权

US10520398B2 Micromanipulator for a cryomicrotome 有权
公开(公告)号：US10520398B2
公开(公告)日：2019-12-31
申请号：US14991631
申请日：2016-01-08
申请人： Leica Mikrosysteme GmbH
发明人： Reinhard Lihl , Michael Zimmermann
IPC分类号： G01N1/06 , B26D1/10 , B26D1/00 , G01N1/42 , B26D1/157
摘要： A microtome system has a cryomicrotome with a sectioning device in which is provided a preparation holder and a knife edge that are guided past one another inside a working space during a sectioning operation, in order to produce thin sections of a preparation retained in the preparation holder. A micromanipulator is operable outside the working space. With the micromanipulator, a tool for retention of a specimen support is positioned proximate the knife edge during a sectioning operation in order to receive the sections that are produced, preferably for substantially stationary retention of the specimen support.

10. 发明申请

US20160030941A1 MANIPULATION HOLDER FOR CRYOMICROSCOPY 审中-公开
标题翻译：手术器械用于结缔组织
公开(公告)号：US20160030941A1
公开(公告)日：2016-02-04
申请号：US14804942
申请日：2015-07-21
申请人： Leica Mikrosysteme GmbH , European Molecular Biology Laboratory
发明人： Reinhard LIHL , Leander GAECHTER , John BRIGGS , Martin SCHORB
IPC分类号： B01L9/00
CPC分类号： B01L9/00 , B01L7/50 , B01L9/50 , B01L2200/025 , B01L2200/087 , B01L2200/185 , B01L2300/041 , B01L2300/0609
摘要： In a manipulation container (1) for cryomicroscopy, having at least one holder (9) for a sample carrier mount (7) and at least one manipulation opening (2) for manipulating sample carriers or sample carrier mounts (7), the holder (9) for a sample carrier mount (7) is movable into a displacement path (11), passing completely through the manipulation container (1), of a displacement apparatus (3) for the sample carrier mount (7).
摘要翻译：在用于冷冻显微镜的操作容器（1）中，具有至少一个用于样品载体安装件（7）的保持器（9）和用于操纵样品载体或样品载体安装件（7）的至少一个操纵开口（2）用于样品载体安装件（7）的移动装置（7）可移动到位移路径（11）中，完全穿过操作容器（1），用于样品载体安装件（7）的位移设备（3）。

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