专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20110159497A1 FREEZE-DRIED COMPOSITIONS FOR CARRYING OUT PCR AND OTHER BIOCHEMICAL REACTIONS 审中-公开
标题翻译：用于携带PCR和其他生物化学反应的冻干组合物
公开(公告)号：US20110159497A1
公开(公告)日：2011-06-30
申请号：US12664183
申请日：2008-06-16
申请人： Martin Alan Lee , Jennifer Mitchell , Emma Henderson
发明人： Martin Alan Lee , Jennifer Mitchell , Emma Henderson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2527/125
摘要： A composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, (ii) a glass forming agent, (iii) a stabilising agent therefore and (iv) fish gelatine. In particular compositions for carrying out PCR are foreseen. Kits comprising these compositions and methods for using them form a further aspect of the invention.
摘要翻译：一种用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含进行所述化学或生物化学反应所需的至少一些化学或生物化学试剂，（ii）），玻璃形成剂，（iii）稳定剂，和（iv）鱼明胶。特别地，预期用于进行PCR的组合物。包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。

2. 发明申请

US20100184059A1 COMPOSITIONS 审中-公开
标题翻译：组合物
公开(公告)号：US20100184059A1
公开(公告)日：2010-07-22
申请号：US12664175
申请日：2008-06-16
申请人： Martin Alan Lee , Jennifer Mitchell , Gerald Adams , Valerie Adams
发明人： Martin Alan Lee , Jennifer Mitchell , Gerald Adams , Valerie Adams
IPC分类号： C12Q1/68 , G01N21/76
CPC分类号： C12N9/96
摘要： A composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried form and comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessary for conducting said chemical or biochemical reaction, including at least one reagent which is fluorescent (ii) a glass forming agent, and (iii) threonine. Kits comprising these compositions and methods of using them form a further aspect of the invention.
摘要翻译：一种用于进行化学或生物化学反应的组合物，所述组合物为冷冻干燥形式，并且包含（i）一组试剂，其包含进行所述化学或生物化学反应所需的至少一些化学或生物化学试剂，包括在至少一种荧光试剂（ii）玻璃形成剂，和（iii）苏氨酸。包含这些组合物的组合物和使用它们的方法形成本发明的另一方面。

3. 发明申请

US20080314855A1 Liquid Dispensing Device with a Cap and a Diaphragm 有权
标题翻译：具有盖和隔膜的液体分配装置
公开(公告)号：US20080314855A1
公开(公告)日：2008-12-25
申请号：US12065659
申请日：2006-09-05
申请人： Martin Alan Lee , David James Squirrell , Georgina Martin
发明人： Martin Alan Lee , David James Squirrell , Georgina Martin
IPC分类号： B65D39/00 , B67D5/06 , B67B7/00
CPC分类号： B01L3/021 , B01L2400/0481 , G01N35/1002 , G01N2035/103
摘要： A liquid dispensing device comprising a hollow body having an opening in a lower end thereof for receiving liquid, and an integrated cap member arranged to sealingly close the body, said cap member comprising a resilient diaphragm which is deformable in a downwards direction. The device is suitable for use in automated apparatus.
摘要翻译：一种液体分配装置，包括在其下端具有用于接收液体的开口的中空本体，以及设置成密封地封闭所述主体的集成的盖构件，所述盖构件包括可沿向下方向变形的弹性隔膜。该设备适用于自动化设备。

4. 发明申请

US20080290292A1 Fluorescence-Based Detection Methods and Apparatus 失效
标题翻译：基于荧光的检测方法和装置
公开(公告)号：US20080290292A1
公开(公告)日：2008-11-27
申请号：US12095444
申请日：2006-12-11
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： G01J1/58
CPC分类号： G01N21/6428 , G01N21/645 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6441 , G01N2201/0691
摘要： In apparatus for detecting the emission of fluorescent radiation from a sample in response to excitation by two or more different radiation sources (145, 160) over the same time period, each radiation source (145, 160) is imprinted with a modulation regime which can then be separately detected in the emitted fluorescent radiation. For instance in techniques based on fluorescent resonant energy transfer (“FRET”), the drive current to two or more different LED sources (145, 160) may be frequency modulated, or pulse width modulated, according to different modulation regimes. Responses of the sample, for instance of different donor/acceptor probes contained in the sample, to each of the sources (145, 160) can then be separately detected by means of the different modulation regimes, even where the wavelengths or wavelength ranges of the responses are the same or overlapping.
摘要翻译：在用于响应于两个或更多个不同的辐射源（145,160）在相同时间段的激发来检测来自样品的荧光辐射的发射的装置中，每个辐射源（145,160）被印刷有调制方式，其可以然后在发射的荧光辐射中单独检测。例如，在基于荧光共振能量转移（“FRET”）的技术中，可以根据不同的调制方式对两个或更多个不同的LED源（145,160）的驱动电流进行频率调制或脉冲宽度调制。然后可以通过不同的调制方式单独地检测样品（例如包含在样品中的不同施主/受体探针）到每个源（145,160）的响应，即使其中波长或波长范围回应是相同或重叠的。

5. 发明授权

US07015018B1 Amplification method for detection of target nucleic acids involving-fluorescence energy transfer 失效
标题翻译：用于检测涉及荧光能量转移的靶核酸的扩增方法
公开(公告)号：US07015018B1
公开(公告)日：2006-03-21
申请号：US10048752
申请日：2000-08-03
申请人： Martin Alan Lee , Dario Lyall Leslie
发明人： Martin Alan Lee , Dario Lyall Leslie
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6853 , C12Q1/6818 , C12Q2531/113 , C12Q2531/131 , C12Q2565/101
摘要： A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is labelled with a first label, and a reagent comprising an amplification primer which can hybridise to said target sequence when in single stranded form and which is connected at its 5′ end to a probe which carries a second label by way of a chemical linking group, said labelled probe being of a sequence which is similar to that of the said target sequence, such that it can hybridise to a complementary region in an amplification product, and wherein one of the first or the second label comprises a donor label and the other comprises an acceptor label, the donor label comprising a fluorescent molecule which is able to donate fluorescent energy to the acceptor label; and monitoring fluorescence of said sample. The method can be used to quantitate the amount of target nucleic acid in the sample as well as to determine sequence characteristics. Kits for effecting the method are also claimed.
摘要翻译：一种用于检测样品中靶核酸序列的存在的方法，所述方法包括使用一组核苷酸对所述样品进行扩增反应，所述核苷酸中的至少一个用第一标记物标记，并且试剂包含扩增当单链形式可以与所述靶序列杂交并且其在5'末端连接到通过化学连接基团携带第二标记的探针的引物时，所述标记的探针具有与之相似的序列的所述靶序列，使得其可以与扩增产物中的互补区域杂交，并且其中所述第一或第二标签中的一个包含供体标记，另一个包含受体标签，所述供体标签包含荧光分子，所述荧光分子能够将荧光能量捐赠给受体标签; 并监测所述样品的荧光。该方法可用于定量样品中靶核酸的量以及确定序列特征。还要求用于实现该方法的套件。

6. 发明授权

US08802031B2 Liquid dispensing device with a cap and a diaphragm 有权
标题翻译：具有盖和隔膜的液体分配装置
公开(公告)号：US08802031B2
公开(公告)日：2014-08-12
申请号：US12065659
申请日：2006-09-05
申请人： Martin Alan Lee , David James Squirrell , Georgina Martin
发明人： Martin Alan Lee , David James Squirrell , Georgina Martin
IPC分类号： B01L3/02 , B01L3/00
CPC分类号： B01L3/021 , B01L2400/0481 , G01N35/1002 , G01N2035/103
摘要： A liquid dispensing device comprising a hollow body having an opening in a lower end thereof for receiving liquid, and an integrated cap member arranged to sealingly close the body, said cap member comprising a resilient diaphragm which is deformable in a downwards direction. The device is suitable for use in automated apparatus.
摘要翻译：一种液体分配装置，包括在其下端具有用于接收液体的开口的中空本体，以及设置成密封地封闭所述主体的集成的盖构件，所述盖构件包括可沿向下方向变形的弹性隔膜。该设备适用于自动化设备。

7. 发明申请

US20100297707A1 REACTION VESSEL COMPRISING CONDUCTIVE LAYER AND INNER NON-METALLIC LAYER 有权
标题翻译：包含导电层和内部非金属层的反应容器
公开(公告)号：US20100297707A1
公开(公告)日：2010-11-25
申请号：US12671272
申请日：2008-08-01
申请人： Martin Alan Lee , David James Squirrell , Ross Peter Jones , Roger James Williamson , George Richard Gregory , Graham Gutsell
发明人： Martin Alan Lee , David James Squirrell , Ross Peter Jones , Roger James Williamson , George Richard Gregory , Graham Gutsell
IPC分类号： C12P19/34 , C12M1/00
CPC分类号： B01L7/52 , B01L3/50851 , B01L2300/0858 , B01L2300/0887 , B01L2300/16 , B01L2300/1827 , B01L2300/1883 , G01N21/0332
摘要： A reaction vessel for conducting a chemical or biochemical reaction, such as a polymerase chain reaction wherein at least one wall of said vessel comprises a metallic layer and an inner non-metallic layer. Reaction systems comprising combinations of vessels of the invention and apparatus for heating them, as well as particular reactions vessels are also described and claimed.
摘要翻译：一种用于进行化学或生物化学反应（例如聚合酶链式反应）的反应容器，其中所述容器的至少一个壁包括金属层和内部非金属层。还描述和要求保护包括本发明的容器和用于加热它们的装置的组合的反应系统以及特定的反应容器。

8. 发明授权

US07700275B2 Detection system 失效
标题翻译：检测系统
公开(公告)号：US07700275B2
公开(公告)日：2010-04-20
申请号：US10478788
申请日：2002-05-24
申请人： Martin Alan Lee
发明人： Martin Alan Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2565/101 , C12Q2561/113
摘要： A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: performing nucleic acid amplification on the sample in the presence of (a) a DNA duplex binding agent, (b) a nucleic acid polymerase and (c) a reagent comprising an amplification primer which can hybridize to said target sequence when in single stranded form and which is connected at its 5′ end to a probe which carries a label by way of a chemical linking group, said labeled probe being of a sequence which is similar to that of the said target nucleic acid sequence, such that it can hybridize to a complementary region in an amplification product, and wherein the label is able to absorb fluorescence from or donate fluorescent energy to the DNA duplex binding agent; and monitoring fluorescence of said sample.
摘要翻译：一种用于检测样品中靶核酸序列的存在的方法，所述方法包括：在（a）DNA双链体结合剂，（b）核酸聚合酶和（c ）包含扩增引物的试剂，所述扩增引物可以单链形式与所述靶序列杂交，并且其在其5'端与通过化学连接基团携带标记的探针连接，所述标记的探针是序列其与所述靶核酸序列类似，使得其可以与扩增产物中的互补区杂交，并且其中所述标记能够从所述DNA双链体结合剂吸收荧光或向其中提供荧光能; 并监测所述样品的荧光。

9. 发明授权

US07537927B2 Reaction system for thermal cycling 失效
标题翻译：热循环反应系统
公开(公告)号：US07537927B2
公开(公告)日：2009-05-26
申请号：US11782255
申请日：2007-07-24
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12M1/00 , C12M3/00
CPC分类号： B01L7/52
摘要： Method and apparatus for carrying out a thermal cycling reaction, wherein a succession of samples is conveyed through a series of sequentially arranged temperature control sites, each of the sites comprising means for supplying an electric current to, or inducing an electric current in, sample-containing vessels passing through it so as to induce temperature changes in the samples. Also provided is a sample support and its production, the support comprising a succession of sample vessels arranged sequentially one behind the next, preferably in the form of a linked chain, the support comprising an electrically conducting, preferably plastics, material which heats when an electric current passes through it.
摘要翻译：用于进行热循环反应的方法和装置，其中一系列样品被传送通过一系列顺序排列的温度控制位点，每个位置包括用于向样品中提供电流或引起电流的装置，通过其容纳的容器以引起样品中的温度变化。还提供了一种样品支持体及其生产，该支撑体包括一系列连续的样品容器，这些样品容器顺序地布置在下一个之后，优选地以连接的链条的形式，该支撑体包括导电的，优选塑料的材料，其在电电流通过它。

10. 发明申请

US20080166700A1 Method For Detecting Viable Cells In A Sample By Using A Virus 有权
标题翻译：通过使用病毒检测样品中活细胞的方法
公开(公告)号：US20080166700A1
公开(公告)日：2008-07-10
申请号：US11816331
申请日：2006-02-16
申请人： David James Squirrell , Martin Alan Lee
发明人： David James Squirrell , Martin Alan Lee
IPC分类号： C12Q1/70 , C12N7/00
CPC分类号： C12Q1/689 , C12Q1/70
摘要： A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; (ii) detecting any nucleic acid obtained by replication of the virus in said cell.
摘要翻译：一种用于检测样品内的活细胞如细菌细胞的方法，所述方法包括（i）在允许所述病毒在任何这样的细胞内感染和复制的条件下，使能够感染所述细胞的病毒孵育所述样品，所述病毒是可行的（ii）检测通过在所述细胞中复制病毒获得的任何核酸。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式