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1. 发明申请

US20060228704A1 Visualization of introduced dna (void) in transit by in situ hybridization 审中-公开
标题翻译：通过原位杂交可视化引入的dna（空白）
公开(公告)号：US20060228704A1
公开(公告)日：2006-10-12
申请号：US10510034
申请日：2003-04-01
申请人： Shen Pan
发明人： Shen Pan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12N15/63 , C12Q2561/113
摘要： The invention relates to a process for monitoring exogenous nucleic acid in transit, termed Visualization Of Introduced DNA (VOID). Once the nucleic acid has been introduced into a cell in a biological sample, the cells are fixed and permeabilized if necessary, then subjected to an in situ hybridization procedure in which the fixed cells are contacted with a probe which hybridizes to the exogenous nucleic acid. The exogenous nucleic acid, in transit, can thus be visualized. VOID may be used (a) to determine the efficiency of delivery of the nucleic acid into the nucleus; (b) to assess the risk associated with DNA delivery procedures where, by tracking the fate of the DNA, it can be determined whether too many of the exogenous DNA are delivered, which may lead to an undesired consequence; (c) to control the copy number of DNA delivery during the development of a transgenic product or a product containing an exogenous nucleic acid as the active ingredient; (d) to identify cells as having been transformed or transfected with an exogenous DNA, without the use of selection markers or reporters; (e) to identify molecular markers associated with transformation competency of a cell and identifying a cell that is competent to receive exogenous nucleic acid; (f) identifying, characterizing and producing cells competent to receive exogenous nucleic acid. VOID has been used successfully to identify the cellular protein VirD2-Interacting protein (VDI) as such a molecular marker. Thus a cell may be identified as being transformation-competent if and when it expresses VDI.
摘要翻译：本发明涉及用于监测转运中的外源核酸的方法，称为引入DNA的可视化（VOID）。一旦将核酸引入生物样品中的细胞中，如果需要，将细胞固定并透化，然后进行原位杂交程序，其中固定的细胞与与外源核酸杂交的探针接触。因此，在运输中的外源核酸可以被可视化。可以使用VOID（a）来确定将核酸递送到细胞核中的效率; （b）评估与DNA递送程序相关的风险，其中通过跟踪DNA的命运，可以确定是否传递了太多的外源DNA，这可能导致不期望的后果; （c）控制在开发转基因产物或含有外源核酸的产物作为活性成分的DNA递送的拷贝数; （d）将细胞鉴定为已经用外源DNA转化或转染，而不使用选择标记物或记者; （e）鉴定与细胞的转化能力相关的分子标记并鉴定能够接受外源核酸的细胞; （f）识别，表征和产生有能力接受外源核酸的细胞。 VOID已成功用于鉴定细胞蛋白VirD2相互作用蛋白（VDI）作为分子标记。因此，如果和表达VDI，则可以将小区识别为具有转型能力的小区。

2. 发明授权

US06447490B1 Vagina cleaning system for preventing pregnancy and sexually transmitted diseases 有权
标题翻译：用于预防怀孕和性传播疾病的阴道清洁系统
公开(公告)号：US06447490B1
公开(公告)日：2002-09-10
申请号：US09573014
申请日：2000-05-17
申请人： James Zhou Liu , Shen Pan
发明人： James Zhou Liu , Shen Pan
IPC分类号： A61M3100
CPC分类号： A61M3/0262 , A61M3/0279 , A61M3/0291
摘要： The present invention discloses an improved vagina cleaning system. The improved system comprises a vagina opener, a vagina cleaning-solution sprayer, a multiple-functional vagina cleaning solution, and a health-promoting bacteria introducer. The system is used to remove semen from the vagina to prevent pregnancy, to reduce pathogenic microorganisms in the vagina to prevent sexually transmitted diseases, to remove other unwanted materials from the vagina to eliminate discomfort, and to add health-promoting bacteria into the vagina to prevent abnormal colonization of microflora in the vaginal cavity. The present invention also discloses methods of using the system for cleaning the vaginal cavity and for introducing health-promoting bacteria into the vaginal cavity.
摘要翻译：本发明公开了一种改良的阴道清洗系统。改进的系统包括阴道开放器，阴道清洁溶液喷雾器，多功能阴道清洁溶液和健康促进细菌导入器。该系统用于从阴道中除去精液以防止怀孕，减少阴道中的致病微生物以预防性传播疾病，从阴道去除其他不需要的物质以消除不适，并将健康促进细菌添加到阴道中防止阴道腔中菌群的异常定植。本发明还公开了使用该系统来清洁阴道腔并将健康促进细菌引入阴道腔的方法。

3. 发明授权

US08965861B1 Concurrency control in database transactions 有权
标题翻译：数据库事务中的并发控制
公开(公告)号：US08965861B1
公开(公告)日：2015-02-24
申请号：US13536411
申请日：2012-06-28
申请人： Zachary M. Shalla , Ullas Sankhla , Shen Pan , Nagesh V. Honnalli , Surendra Moolchand Bashani , Vaibhav Sharma , Gaurav D. Ghare , Sushanth Tiruvaipati , Léon Thrane
发明人： Zachary M. Shalla , Ullas Sankhla , Shen Pan , Nagesh V. Honnalli , Surendra Moolchand Bashani , Vaibhav Sharma , Gaurav D. Ghare , Sushanth Tiruvaipati , Léon Thrane
IPC分类号： G06F17/30
CPC分类号： G06F17/30362 , G06F9/466 , G06F9/546 , G06F17/30348 , G06F17/30371 , G06F17/30489
摘要： To achieve long running batch updates, a transaction aggregator layer and one or more data aggregation queues, such as a command queue, are placed between one or more clients and a database. Writes go to the database aggregator layer and are enqueued in the appropriate data aggregation queue, which may be allocated by transaction. Committing the transaction drains the queue and writes the contents of the queue in one transaction-like update to the database. By using the transaction logic in the transaction aggregator layer, transaction-like behavior may be achieved that allows both updates and transactions to scale with less impact on database servers.
摘要翻译：为了实现长时间运行的批量更新，将事务聚合器层和一个或多个数据聚合队列（如命令队列）放置在一个或多个客户端和数据库之间。写入数据库聚合器层，并排入适当的数据聚合队列，可以由事务分配。提交事务排除队列，并将队列的内容写入到类似于事务的更新中。通过在事务聚合器层中使用事务逻辑，可以实现类似事务的行为，允许更新和事务在对数据库服务器的影响较小的情况下扩展。

4. 外观设计

USD521987S1 Headset 失效
公开(公告)号：USD521987S1
公开(公告)日：2006-05-30
申请号：US29199536
申请日：2004-02-17
申请人： Jeong J. Ma , Donald Y. Tio , Hung Shen Pan
设计人： Jeong J. Ma , Donald Y. Tio , Hung Shen Pan

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