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1. 发明授权

US12005436B2 Devices and methods for separating particles from a liquid 有权
公开(公告)号：US12005436B2
公开(公告)日：2024-06-11
申请号：US16615877
申请日：2018-05-25
申请人： Stemcell Technologies Inc.
发明人： Eric Jervis , Tom Glawdel
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01L3/5082 , B01L2300/0858 , B01L2400/086
摘要： Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface.

2. 发明授权

US10961506B2 Soluble antibody complexes for T cell or NK cell activation and expansion 有权
公开(公告)号：US10961506B2
公开(公告)日：2021-03-30
申请号：US14844717
申请日：2015-09-03
申请人： StemCell Technologies Inc.
发明人： Andy Isamu Kokaji
IPC分类号： C12N5/0783 , C07K14/725 , C07K14/705
摘要： This disclosure provides compositions and methods for the activation and expansion of human T cells or NK cells using soluble monospecific antibody complexes.

3. 发明授权

US10081793B2 Method for separating cells using immunorosettes and magnetic particles 有权
公开(公告)号：US10081793B2
公开(公告)日：2018-09-25
申请号：US14775987
申请日：2014-03-12
申请人： Stemcell Technologies Inc.
发明人： Andy Isamu Kokaji
IPC分类号： C12N5/078 , G01N33/569 , G01N33/543 , G01N1/40 , A23L2/52 , A61K31/122 , A61K31/355 , A61K31/375 , A61K31/4188 , A61K31/51 , A61K31/525 , A61K31/593 , A61K31/714 , A61K33/06 , A61K33/30 , A61K31/07 , A61K31/197 , A61K31/198 , A61K31/221 , A61K31/385 , A61K31/4415 , A61K31/455 , A61K31/519 , A61K33/00 , A61K33/04 , A23L33/10 , A23L33/105 , A23L33/12 , A23L33/15 , A23L33/155 , A23L33/16 , A23L33/175
CPC分类号： C12N5/0634 , A23L2/52 , A23L33/10 , A23L33/105 , A23L33/12 , A23L33/15 , A23L33/155 , A23L33/16 , A23L33/175 , A61K31/07 , A61K31/122 , A61K31/197 , A61K31/198 , A61K31/221 , A61K31/355 , A61K31/375 , A61K31/385 , A61K31/4188 , A61K31/4415 , A61K31/455 , A61K31/51 , A61K31/519 , A61K31/525 , A61K31/593 , A61K31/714 , A61K33/00 , A61K33/04 , A61K33/06 , A61K33/30 , A61K2300/00 , C07K16/28 , C07K16/2896 , C07K16/4283 , C07K2317/31 , G01N1/40 , G01N33/54326 , G01N33/56966 , G01N33/56972
摘要： The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.

4. 发明申请

US20160032248A1 COMPOSITIONS AND METHODS FOR OBTAINING ENRICHED MESENCHYMAL STEM CELL CULTURES 有权
标题翻译：用于获得富集的细胞干细胞培养物的组合物和方法
公开(公告)号：US20160032248A1
公开(公告)日：2016-02-04
申请号：US14776866
申请日：2014-03-12
申请人： STEMCELL TECHNOLOGIES INC.
发明人： Brenton John SHORT , Christopher Duronio
IPC分类号： C12N5/0775
CPC分类号： C12N5/0663 , C12N5/0668 , C12N2501/727 , C12N2501/999
摘要： The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
摘要翻译：本公开提供了培养间充质细胞谱系的细胞的方法，所述方法包括使细胞与包含CSF1R激酶抑制剂的培养基接触。本公开还提供了从骨髓和/或紧密骨培养细胞以使细胞与间充质细胞系的细胞富集的方法，包括使细胞与包含CSF1R激酶抑制剂的培养基接触。还提供了包含CSF1R激酶抑制剂并且可用于培养间充质细胞谱系细胞和/或富含间充质细胞系的细胞的细胞培养基。

5. 发明申请

US20150352546A1 MENISCUS REDUCING MEMBER 有权
标题翻译：男士减少会员
公开(公告)号：US20150352546A1
公开(公告)日：2015-12-10
申请号：US14760397
申请日：2014-01-10
申请人： STEMCELL TECHNOLOGIES INC.
发明人： Oliver Egeler , Steven Woodside
IPC分类号： B01L3/00 , B05D1/18 , B05D5/08 , B05D1/00
CPC分类号： B01L3/508 , B01L3/5085 , B01L2200/06 , B01L2300/0829 , B01L2300/0858 , B01L2300/16 , B01L2300/161 , B01L2300/166 , B05D1/002 , B05D1/18 , B05D5/08 , C08G77/24 , C09D183/08
摘要： A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.
摘要翻译：用于容纳液体的容器中的弯液面减少构件可包括覆盖容器的内表面的至少一部分的物理表面特征。物理表面特征可以具有大致平行的第一和第二内表面以及在第一和第二表面之间延伸的至少第三表面。第一内表面，第二内表面和第三表面可以被配置为物理地改变液体和物理表面特征之间的后退接触角。可以将涂层材料施加到物理表面特征的至少一个表面，以化学地改变液体和涂覆表面之间的后退接触角，由此在液体和弯液面减少构件之间形成的后退接触角在约75 度和110度。

6. 发明申请

US20150204765A1 Method For Separating Target Entities From A Sample Using A Composition of Mono-Specific Tetrameric Antibody Complexes Coupled to A Surface 有权
标题翻译：使用与表面偶联的单特异性四聚体抗体复合物的组合物从样品中分离目标实体的方法
公开(公告)号：US20150204765A1
公开(公告)日：2015-07-23
申请号：US14601367
申请日：2015-01-21
申请人： Stemcell Technologies Inc.
发明人： Andy Isamu Kokaji
IPC分类号： G01N1/40
CPC分类号： G01N1/405 , G01N33/543 , G01N33/54326 , G01N33/6854
摘要： An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.
摘要翻译：描述了用于制备靶特定表面的改进方法及其用途。特别地，表面在添加到含有目标实体的样品并将其与非目标实体分离之前，与单特异性四聚体抗体复合物结合。

7. 发明授权

US10537891B2 Meniscus reducing member 有权
公开(公告)号：US10537891B2
公开(公告)日：2020-01-21
申请号：US15918798
申请日：2018-03-12
申请人： STEMCELL TECHNOLOGIES INC
发明人： Oliver Egeler , Steven Woodside
IPC分类号： B01L3/00
摘要： A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

8. 发明申请

US20180200711A1 MENISCUS REDUCING MEMBER 审中-公开
公开(公告)号：US20180200711A1
公开(公告)日：2018-07-19
申请号：US15918798
申请日：2018-03-12
申请人： STEMCELL TECHNOLOGIES INC
发明人： Oliver Egeler , Steven Woodside
IPC分类号： B01L3/00
CPC分类号： B01L3/508 , B01L3/5085 , B01L2200/026 , B01L2300/0829 , B01L2300/0851 , B01L2300/0858 , B01L2300/165 , B01L2300/166 , C12M1/00 , C12M23/04 , C12M23/12 , C12M23/20 , C12M41/36
摘要： A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

9. 发明授权

US10017741B2 Compositions and methods for obtaining enriched mesenchymal stem cell cultures 有权
公开(公告)号：US10017741B2
公开(公告)日：2018-07-10
申请号：US14776866
申请日：2014-03-12
申请人： StemCell Technologies Inc.
发明人： Brenton John Short , Christopher Duronio
IPC分类号： C12N5/0775
摘要： The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.

10. 发明申请

US20150204857A1 Compositions and Methods for Rapid and Reversible Biomolecular Labeling 审中-公开
标题翻译：用于快速和可逆生物分子标记的组合物和方法
公开(公告)号：US20150204857A1
公开(公告)日：2015-07-23
申请号：US14419665
申请日：2013-08-22
申请人： STEMCELL TECHNOLOGIES INC.
发明人： Samuel Jon Clarke
IPC分类号： G01N33/533 , G01N33/569 , G01N33/543
CPC分类号： G01N33/533 , B01D15/265 , B82Y5/00 , G01N33/54306 , G01N33/56966 , G01N33/58 , G01N2333/7155
摘要： This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.
摘要翻译：本公开提供了在生理条件下快速逆转的低亲合力，高亲和力和高特异性生物分子相互作用的组合物和方法。所述方法包括将生物学靶标（例如分子，蛋白质，DNA，细胞等）与聚合物和抗聚合物配体连接，以及使用生理上相容的聚合化合物来逆转其结合的方法。所述方法还包括将不同聚合物/抗聚合物体系结合以进行正交标记的方法。组合物包含与聚合物或缀合至抗聚合物抗体的标记缀合的颗粒（荧光，磁性，致密性等）的标记。组合物还包含与聚合物缀合的生物分子（蛋白质，抗体，DNA等）。这些方法和组成代表了对最先进技术的重大改进。它们对于使用颗粒的生物靶标的分离和分离特别有用，但在包括荧光成像在内的其他领域具有重要应用。

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