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1. 发明申请

US20130212717A1 METHOD FOR PRODUCING PENTAMERIC CRP, PENTAMERIC CRP-PRODUCING TRANSGENIC SILKWORM AND METHOD FOR CONSTRUCTING SAME, DNA ENCODING CANINE MONOMERIC CRP AND EXPRESSION VECTOR CONTAINING THE DNA 有权
标题翻译：生产PENTAMERIC CRP，PENTAMERIC CRP生产转基因丝状细胞的方法及其构建方法，DNA编码犬单核细胞CRP和含有DNA的表达载体
公开(公告)号：US20130212717A1
公开(公告)日：2013-08-15
申请号：US13701946
申请日：2011-05-30
申请人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
发明人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
IPC分类号： C12N15/85 , A01K67/033
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/052 , A01K2227/706 , A01K2267/01 , C12N15/09 , C12P21/02
摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
摘要翻译：通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。

2. 发明申请

US20110239313A1 METHOD FOR PRODUCTION OF TRACP5b 有权
标题翻译：生产TRACP5b的方法
公开(公告)号：US20110239313A1
公开(公告)日：2011-09-29
申请号：US12521701
申请日：2007-12-28
申请人： Iwao Kiyokawa , Tatsuya Ohashi , Toshihide Miura , Katsuhiro Katayama , Toshiki Tamura , Isao Kobayashi , Hideki Sezutsu
发明人： Iwao Kiyokawa , Tatsuya Ohashi , Toshihide Miura , Katsuhiro Katayama , Toshiki Tamura , Isao Kobayashi , Hideki Sezutsu
IPC分类号： C12P21/00 , C12N15/85 , A01K67/04
CPC分类号： C12N9/16
摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.
摘要翻译：蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA）生产。结果表明，活性TRACP5b由蚕生产。这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

3. 发明申请

US20110021757A1 TRANSGENIC SILKWORM CAPABLE OF PRODUCING ANTIBODY AND METHOD FOR PRODUCTION THEREOF 有权
标题翻译：可生产抗体的转基因丝和其生产方法
公开(公告)号：US20110021757A1
公开(公告)日：2011-01-27
申请号：US12090702
申请日：2006-10-18
申请人： Toshiki Tamura , Isao Kobayashi , Toshio Kanda , Keiro Uchino , Katsuhiro Katayama , Tatsuya Ohashi , Iwao Kiyokawa , Hisae Arai , Noriyuki Funahashi
发明人： Toshiki Tamura , Isao Kobayashi , Toshio Kanda , Keiro Uchino , Katsuhiro Katayama , Tatsuya Ohashi , Iwao Kiyokawa , Hisae Arai , Noriyuki Funahashi
IPC分类号： C07K16/00 , C12P21/00 , C07H21/04 , C12N15/63 , C12N5/10 , A01K67/00
CPC分类号： C07K16/00 , A01K67/0335 , A01K67/04 , A01K2217/05 , A01K2227/703 , A01K2267/01 , C07K14/43586 , C07K2317/10 , C07K2317/20 , C07K2319/02 , C12N15/8509 , C12N2830/002 , C12N2830/003
摘要： The present inventors produced transgenic silkworms which comprise a promoter of a DNA encoding a protein specifically expressed in the silk gland and a DNA encoding a recombinant antibody whose expression is regulated directly or indirectly by the promoter, and which secrete the recombinant antibody into the silk gland. The recombinant antibodies produced from the silk gland of the transgenic silkworms were confirmed to be active.
摘要翻译：本发明人生产了转基因蚕，其包含编码丝腺特异性表达的蛋白质的DNA的启动子和编码重组抗体的DNA，其表达由启动子直接或间接调节，并将重组抗体分泌到丝腺中。从转基因蚕丝腺产生的重组抗体被证实是活性的。

4. 发明申请

US20050177877A1 Process for producing physiologically active protein using genetically modified silkworm 失效
标题翻译：使用转基因蚕生产生理活性蛋白的方法
公开(公告)号：US20050177877A1
公开(公告)日：2005-08-11
申请号：US10506327
申请日：2003-03-06
申请人： Shingo Hiramatsu , Takashi Tanaka , Katsushige Yamada , Toshiki Tamura
发明人： Shingo Hiramatsu , Takashi Tanaka , Katsushige Yamada , Toshiki Tamura
IPC分类号： A01K67/033 , A01K67/04 , C07K14/52 , C12N15/85 , C12N5/06
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/05 , A01K2267/01 , C07K14/52
摘要： The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.
摘要翻译：本发明提供了一种用于昆虫的遗传工程材料，其能够容易地纯化靶蛋白，而不需要使用重组杆状病毒，同时提供使用该遗传工程材料生产外源蛋白的方法。通过将外源蛋白质基因例如与在丝腺中起作用的启动子偶联的细胞因子基因插入蚕染色体而获得基因重组蚕。然后从蚕丝或其后代的丝腺或茧中提取和纯化外源蛋白，如细胞因子。通过插入表达基因盒，可以在丝腺细胞，丝腺细胞外或丝线或茧中产生大量外源蛋白，其中3'末端部分的DNA序列和5'端的DNA序列 “丝心蛋白H链基因的末端部分融合到外源蛋白基因，进入丝腺细胞等。

5. 发明授权

US08952215B2 Antibody-producing transgenic silkworms and methods for producing the same 有权
标题翻译：产生抗体的转基因蚕及其制备方法
公开(公告)号：US08952215B2
公开(公告)日：2015-02-10
申请号：US12090702
申请日：2006-10-18
申请人： Toshiki Tamura , Isao Kobayashi , Toshio Kanda , Keiro Uchino , Katsuhiro Katayama , Tatsuya Ohashi , Iwao Kiyokawa , Hisae Arai , Noriyuki Funahashi
发明人： Toshiki Tamura , Isao Kobayashi , Toshio Kanda , Keiro Uchino , Katsuhiro Katayama , Tatsuya Ohashi , Iwao Kiyokawa , Hisae Arai , Noriyuki Funahashi
IPC分类号： C12N15/00 , C07H21/04 , C07K16/00 , A01K67/033 , A01K67/04 , C07K14/435 , C12N15/85
CPC分类号： C07K16/00 , A01K67/0335 , A01K67/04 , A01K2217/05 , A01K2227/703 , A01K2267/01 , C07K14/43586 , C07K2317/10 , C07K2317/20 , C07K2319/02 , C12N15/8509 , C12N2830/002 , C12N2830/003
摘要： The present inventors produced transgenic silkworms which comprise a promoter of a DNA encoding a protein specifically expressed in the silk gland and a DNA encoding a recombinant antibody whose expression is regulated directly or indirectly by the promoter, and which secrete the recombinant antibody into the silk gland. The recombinant antibodies produced from the silk gland of the transgenic silkworms were confirmed to be active.
摘要翻译：本发明人生产了转基因蚕，其包含编码丝腺特异性表达的蛋白质的DNA的启动子和编码重组抗体的DNA，其表达由启动子直接或间接调节，并将重组抗体分泌到丝腺中。从转基因蚕丝腺产生的重组抗体被证实是活性的。

6. 发明授权

US07659112B2 Process for producing physiologically active protein using genetically modified silkworm 失效
标题翻译：使用转基因蚕生产生理活性蛋白的方法
公开(公告)号：US07659112B2
公开(公告)日：2010-02-09
申请号：US10506327
申请日：2003-03-06
申请人： Shingo Hiramatsu , Takashi Tanaka , Katsushige Yamada , Toshiki Tamura
发明人： Shingo Hiramatsu , Takashi Tanaka , Katsushige Yamada , Toshiki Tamura
IPC分类号： C12N15/00 , C12P21/00
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/05 , A01K2267/01 , C07K14/52
摘要： The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.
摘要翻译：本发明提供了一种用于昆虫的遗传工程材料，其能够容易地纯化靶蛋白，而不需要使用重组杆状病毒，同时提供使用该遗传工程材料生产外源蛋白的方法。通过将外源蛋白质基因例如与在丝腺中起作用的启动子偶联的细胞因子基因插入蚕染色体而获得基因重组蚕。然后从蚕丝或其后代的丝腺或茧中提取和纯化外源蛋白，如细胞因子。通过插入表达基因盒，可以在丝腺细胞，丝腺细胞外或丝线或茧中产生大量外源蛋白，其中3'末端部分的DNA序列和5'端的DNA序列 “丝心蛋白H链基因的末端部分融合到外源蛋白基因，进入丝腺细胞等。

7. 发明申请

US20070083940A1 Transformed silkworm producing human collagen 审中-公开
标题翻译：转化蚕生产人胶原蛋白
公开(公告)号：US20070083940A1
公开(公告)日：2007-04-12
申请号：US10475107
申请日：2001-06-11
申请人： Katsutoshi Yoshizato , Masahiro Tomita , Tsutomu Satou , Hajime Mori , Toshiki Tamura , Takahiro Adachi , Hiroto Munetsuna
发明人： Katsutoshi Yoshizato , Masahiro Tomita , Tsutomu Satou , Hajime Mori , Toshiki Tamura , Takahiro Adachi , Hiroto Munetsuna
IPC分类号： A01K67/033
CPC分类号： C12N15/8509 , A01K67/0333 , A01K67/0339 , A01K2217/05 , A01K2267/01
摘要： The present invention provides a transformed silkworm which has a polynucleotide encoding human collagen within the genomic DNA and produces recombinant human collagen as a part of proteins in the cocoon or the silk gland; a process for producing recombinant human collagen by using this transformed silkworm; and a recombinant vector for use in the generation of the transformed silkworm. Since human collagen is collected from the cocoon discharged by this transformed silkworm or its silk gland, highly pure human collagen can be conveniently obtained in a large amount. Moreover, because the recombinant human collagen produced by the transformed silkworm is safe collagen which is free from any fear of the contamination with pathogens such as viruses or prions and exhibits no antigenicity toward humans. Thus, it can be utilized in various industrial fields including medicines, foods, cosmetics and the like.
摘要翻译：本发明提供了一种转化的蚕，其具有在基因组DNA内编码人胶原蛋白的多核苷酸，并产生作为茧或丝腺中的蛋白质的一部分的重组人胶原蛋白; 通过使用该转化的蚕生产重组人胶原蛋白的方法; 以及用于产生转化的蚕的重组载体。由于由这种转化的蚕丝或其丝腺排出的茧收集人胶原蛋白，所以可以方便地获得高纯度的人胶原蛋白。此外，因为由转化的蚕产生的重组人胶原是安全的胶原蛋白，它不受任何对病原体（例如病毒或朊病毒）的污染的恐惧，并且对人类没有抗原性。因此，可以用于包括药品，食品，化妆品等在内的各种工业领域。

8. 发明申请

US20060070132A1 Methods for producing proteins using silkworm middle silk gland-specific gene expression system 有权
标题翻译：使用蚕丝绸腺特异性基因表达系统生产蛋白质的方法
公开(公告)号：US20060070132A1
公开(公告)日：2006-03-30
申请号：US11085838
申请日：2005-03-21
申请人： Toshiki Tamura , Hideki Sezutsu , Isao Kobayashi , Katsura Kojima , Toshio Kanda , Keiro Uchino
发明人： Toshiki Tamura , Hideki Sezutsu , Isao Kobayashi , Katsura Kojima , Toshio Kanda , Keiro Uchino
IPC分类号： A01K67/033
CPC分类号： C12N15/8509 , A01K67/0333 , A01K67/04 , A01K2217/05 , A01K2227/706 , A01K2267/01 , A01K2267/0393
摘要： Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.
摘要翻译：产生包含GFP的转基因蚕，其表达受丝胶蛋白基因启动子调节。蚕的最后一龄幼虫的丝腺的观察仅在中间的丝腺中显示出荧光。 GFP从旋转阶段周围的中间silkgland细胞分泌，表明GFP移入腺体管腔。最后，将GFP转染成茧丝，制成含有大量GFP的茧。因此，通过使用丝胶蛋白基因的启动子区域，可以在中间丝腺中产生重组蛋白质。此外，在中间丝腺中产生的重组蛋白质容易分泌到中间丝腺的内腔中。

9. 发明授权

US6123572A Modular plug for a signal transmission cable 失效
标题翻译：用于信号传输电缆的模块化插头
公开(公告)号：US6123572A
公开(公告)日：2000-09-26
申请号：US419223
申请日：1999-10-15
申请人： Takuji Ishii , Toshiki Tamura , Masahito Watanabe
发明人： Takuji Ishii , Toshiki Tamura , Masahito Watanabe
IPC分类号： H01R13/50 , H01R13/506 , H01R13/58 , H01R11/20 , H01R13/14
CPC分类号： H01R13/5829 , H01R13/6463 , H01R13/501 , H01R13/506
摘要： A modular plug includes a dielectric housing, a plurality of terminals, and a signal transmission cable. The dielectric housing has a cable insertion end, a mating end opposite to the cable insertion end, a lower body portion, and an upper lid portion connected to the lower body portion. The lower body portion extends from the cable insertion end to the mating end. The lower body portion has a plurality of terminal grooves formed adjacent to the mating end, and a cable receiving space extending from the cable insertion end adjacent to the terminal grooves. The upper lid portion is operable relative to the lower body portion to expose the cable receiving space. The terminals are inserted into the cable receiving space through the terminal grooves. The signal transmission cable is received in the cable receiving space, and has a plurality of conductive wires that extend adjacent to the terminal grooves, respectively, and that are connected electrically to the terminals. The upper lid portion lies over the cable receiving space without extending to the terminal grooves, and has a protrusion projecting into the cable receiving space to press the signal transmission cable against the lower body portion.
摘要翻译：模块化插头包括绝缘壳体，多个端子和信号传输电缆。绝缘壳体具有电缆插入端，与电缆插入端相对的配合端，下主体部分和连接到下主体部分的上盖部分。下主体部分从电缆插入端延伸到配合端。下主体部分具有邻近配合端形成的多个端子槽，以及从电缆插入端部延伸到电缆槽的相邻端子槽的电缆接收空间。上盖部分可相对于下主体部分操作以暴露电缆接收空间。端子通过端子槽插入电缆接收空间。信号传输电缆被接收在电缆接收空间中，并且具有分别与端子槽相邻延伸并且与端子电连接的多条导线。上盖部分位于电缆接收空间之上，而不延伸到端子槽，并且具有突出到电缆接收空间中的突起，以将信号传输电缆按压在下主体部分上。

10. 发明授权

US08865966B2 Pentameric CRP-producing transgenic silkworm 有权
标题翻译：五聚体CRP生产转基因蚕
公开(公告)号：US08865966B2
公开(公告)日：2014-10-21
申请号：US13701946
申请日：2011-05-30
申请人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
发明人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
IPC分类号： C12P21/00 , A01K67/033 , C12N15/00 , C12N15/85 , C12N15/09 , A01K67/04 , C12P21/02
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/052 , A01K2227/706 , A01K2267/01 , C12N15/09 , C12P21/02
摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
摘要翻译：通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。

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