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    • 9. 发明申请
    • NOVEL METHOD FOR THE PRODUCTION OF DIFFERENTIATED RESPIRATORY EPITHELIAL CELLS
    • 用于生产分化呼吸上皮细胞的新方法
    • US20140017691A1
    • 2014-01-16
    • US13983299
    • 2012-02-03
    • Ulrich MartinChristina Mauritz
    • Ulrich MartinChristina Mauritz
    • C12N5/071C12Q1/68G01N33/50
    • C12N5/0688C12N2501/01C12N2501/117C12N2501/39C12Q1/6881C12Q1/6897G01N33/5044
    • The present invention relates to a method for the production of differentiated respiratory epithelial cells comprising: (a) providing a cell population comprising or consisting of precursor cells of respiratory epithelial cells; (b) culturing the cell population of (a) in culture medium to which keratinocyte growth factor has been added; wherein the cultured cell population is supplemented with a glucocorticoid, a cAMP analogue and a cAMP elevating agent and wherein said supplementation is either simultaneously with the addition of keratinocyte growth factor in step (b) or prior or subsequently to the addition of keratinocyte growth factor in step (b), thereby differentiating said precursor cells into respiratory epithelial cells. The present invention further relates to the cell(s) obtained by the method of the invention for use in treating or preventing a respiratory disease and to a method for identifying a compound having an pharmacological, cytotoxic, proliferative, transforming or differentiating effect on the differentiated respiratory epithelial cells obtained by the method of the invention.
    • 本发明涉及产生分化的呼吸上皮细胞的方法,包括:(a)提供包含呼吸上皮细胞的前体细胞或由其组成的细胞群; (b)在已加入角质形成细胞生长因子的培养基中培养(a)的细胞群; 其中所述培养的细胞群体补充有糖皮质激素,cAMP类似物和cAMP升高剂,并且其中所述补充剂或者是在步骤(b)中或加入角化细胞生长因子之前或随后加入角质形成细胞生长因子同时与 步骤(b),从而将所述前体细胞分化成呼吸上皮细胞。 本发明还涉及通过本发明的用于治疗或预防呼吸系统疾病的方法获得的细胞,以及用于鉴别具有药理,细胞毒性,增殖,转化或分化作用的化合物对分化的化合物的方法 通过本发明的方法获得的呼吸上皮细胞。