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1. 发明申请

US20120171739A1 Xylitol Producing Microorganism Introduced with Arabinose Metabolic Pathway and Production Method of Xylitol Using the Same 有权
标题翻译：用阿拉伯糖代谢途径引入的木糖醇生产微生物和使用其的木糖醇的生产方法
公开(公告)号：US20120171739A1
公开(公告)日：2012-07-05
申请号：US13391073
申请日：2011-01-18
申请人： Jung Hoe Kim , Byoung Hoon Yoon , Woo Young Jeon , Woo Yong Shim
发明人： Jung Hoe Kim , Byoung Hoon Yoon , Woo Young Jeon , Woo Yong Shim
IPC分类号： C12P7/18 , C12N1/19
CPC分类号： C12P7/18
摘要： The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose/arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in Candida tropicalis, codon optimization was performed. Then, each gene was inserted in the gene expression cassette containing the glyceraldehyde-3-phosphate dehydrogenase promoter and the selection marker URA3, which was introduced into Candida sp. microorganism. As a result, arabitol, the byproduct interrupting the purification and crystallization of xylitol could be inhibited, making the production method of xylitol of the present invention more efficient. The xylitol producing microorganism introduced with arabinose metabolic pathway of the present invention can be effectively used for the production of xylitol with high productivity by inhibiting the generation of arabitol.
摘要翻译：本发明涉及通过使用引入了阿拉伯糖代谢途径的木糖醇生产微生物来抑制阿糖醇（副产物）的产生的代谢产物，而是仅将木糖/阿拉伯糖混合培养基中的细胞代谢使用阿拉伯糖，从而有效地生产木糖醇。更准确地说，为了在热带假丝酵母中有效地表达L-阿拉伯糖异构酶（araA），L-核糖激酶（araB）和L-核酮糖-5-磷酸4-差向异构酶（araD），进行密码子优化。然后将每个基因插入含有甘油醛-3-磷酸脱氢酶启动子和选择标记URA3的基因表达盒中，将其引入假丝酵母属微生物。结果，可以抑制中和木糖醇的纯化和结晶的副产物，从而使本发明的木糖醇的生产方法更有效。引入本发明的阿拉伯糖代谢途径的木糖醇生产微生物可以通过抑制阿拉伯糖醇的产生而以高生产率有效地用于生产木糖醇。

2. 发明申请

US20230080381A1 EMERGENCY BRAKING DEVICE FOR AUTOMOBILE 有权
公开(公告)号：US20230080381A1
公开(公告)日：2023-03-16
申请号：US17799786
申请日：2021-04-06
申请人： Gwang Seok JEON , Woo Young JEON
发明人： Gwang Seok JEON , Woo Young JEON
IPC分类号： B60T17/04 , B60T17/22 , B60T7/04 , F16K17/02
摘要： An object of the present invention is to provide an emergency braking device for an automobile that is capable of preventing a safety-related accident by applying a braking force even when a brake pipe or the like is ruptured. To this end, the emergency braking device for an automobile of the present invention is an emergency braking device for an automobile that is added to an automobile that is braked by a pressure of main brake fluid transferred through a brake pipe when a brake pedal is stepped on and that is instantaneously and automatically switched to an emergency braking device when braking is not performed due to the rupture of the existing (main) brake pipe, or the like. The emergency braking device for an automobile includes a valve unit, an emergency pipe; a pressing part; and a pressure source.

3. 发明授权

US08343736B2 Xylitol producing microorganism introduced with arabinose metabolic pathway and production method of xylitol using the same 有权
标题翻译：木糖醇生产微生物引入阿拉伯糖代谢途径和木糖醇的生产方法
公开(公告)号：US08343736B2
公开(公告)日：2013-01-01
申请号：US13391073
申请日：2011-01-18
申请人： Jung Hoe Kim , Byoung Hoon Yoon , Woo Young Jeon , Woo Yong Shim
发明人： Jung Hoe Kim , Byoung Hoon Yoon , Woo Young Jeon , Woo Yong Shim
IPC分类号： C12P21/06 , C12N15/19 , C12N1/00 , C12N15/00
CPC分类号： C12P7/18
摘要： The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose/arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in Candida tropicalis, codon optimization was performed. Then, each gene was inserted in the gene expression cassette containing the glyceraldehyde-3-phosphate dehydrogenase promoter and the selection marker URA3, which was introduced into Candida sp. microorganism. As a result, arabitol, the byproduct interrupting the purification and crystallization of xylitol could be inhibited, making the production method of xylitol of the present invention more efficient. The xylitol producing microorganism introduced with arabinose metabolic pathway of the present invention can be effectively used for the production of xylitol with high productivity by inhibiting the generation of arabitol.
摘要翻译：本发明涉及通过使用引入了阿拉伯糖代谢途径的木糖醇生产微生物来抑制阿糖醇（副产物）的产生的代谢产物，而是仅将木糖/阿拉伯糖混合培养基中的细胞代谢使用阿拉伯糖，从而有效地生产木糖醇。更准确地说，为了在热带假丝酵母中有效地表达L-阿拉伯糖异构酶（araA），L-核糖激酶（araB）和L-核酮糖-5-磷酸4-差向异构酶（araD），进行密码子优化。然后将每个基因插入含有甘油醛-3-磷酸脱氢酶启动子和选择标记URA3的基因表达盒中，将其引入假丝酵母属微生物。结果，可以抑制中和木糖醇的纯化和结晶的副产物，从而使本发明的木糖醇的生产方法更有效。引入本发明的阿拉伯糖代谢途径的木糖醇生产微生物可以通过抑制阿拉伯糖醇的产生而以高生产率有效地用于生产木糖醇。

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