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    • 1. 发明授权
    • Use of template switching for DNA synthesis
    • 使用模板切换进行DNA合成
    • US09012183B2
    • 2015-04-21
    • US14000513
    • 2012-02-23
    • Alan M. LambowitzSabine MohrTravis B. WhiteScott Kuersten
    • Alan M. LambowitzSabine MohrTravis B. WhiteScott Kuersten
    • C12P19/34C12N15/10C12Q1/68
    • C12P19/34C12N15/1096C12Q1/6806C12Q2521/107C12Q2525/191C12Q2525/207
    • A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.
    • 描述了使用模板切换来制备靶多核苷酸的DNA拷贝的方法。 该方法包括将由与互补寡核苷酸模板链相关的DNA引物寡核苷酸组成的双链模板/引物底物与靶多核苷酸混合在反应介质中,并向反应介质中加入适量的非逆转录病毒逆转录酶至 从其3'末端延伸DNA引物寡核苷酸以提供DNA拷贝多核苷酸。 DNA拷贝多核苷酸包括使用靶多核苷酸作为模板合成的互补靶DNA多核苷酸。 还描述了向双链模板/底物底物添加核苷酸的方法。 该方法可用于促进RNA和DNA序列的检测,PCR扩增,克隆和测定。
    • 2. 发明申请
    • USE OF TEMPLATE SWITCHING FOR DNA SYNTHESIS
    • 使用模板切换进行DNA合成
    • US20140004569A1
    • 2014-01-02
    • US14000513
    • 2012-02-23
    • Alan M. LambowitzSabine MohrTravis B. WhiteScott Kuersten
    • Alan M. LambowitzSabine MohrTravis B. WhiteScott Kuersten
    • C12P19/34
    • C12P19/34C12N15/1096C12Q1/6806C12Q2521/107C12Q2525/191C12Q2525/207
    • A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.
    • 描述了使用模板切换来制备靶多核苷酸的DNA拷贝的方法。 该方法包括将由与互补寡核苷酸模板链相关的DNA引物寡核苷酸组成的双链模板/引物底物与靶多核苷酸混合在反应介质中,并向反应介质中加入适量的非逆转录病毒逆转录酶至 从其3'末端延伸DNA引物寡核苷酸以提供DNA拷贝多核苷酸。 DNA拷贝多核苷酸包括使用靶多核苷酸作为模板合成的互补靶DNA多核苷酸。 还描述了向双链模板/底物底物添加核苷酸的方法。 该方法可用于促进RNA和DNA序列的检测,PCR扩增,克隆和测定。