专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07670824B2 Multicopy-integration of heterologous genes and expression in Methylobacterium 有权
标题翻译：异源基因的多重整合和在甲基杆菌中的表达
公开(公告)号：US07670824B2
公开(公告)日：2010-03-02
申请号：US11492822
申请日：2006-07-26
申请人： Carlos B. Miguez , Young-Jun Choi
发明人： Carlos B. Miguez , Young-Jun Choi
IPC分类号： C12N1/20 , C12N15/00 , C12N15/74
CPC分类号： C12N15/74
摘要： The integration of genes into methylobacterium, such as M. extorquens ATCC is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (PmxaF). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for M. extorquens.
摘要翻译：使用转座子系统，优选小型Tn7转座子系统，并在启动子（如强甲醇脱氢酶启动子（PmxaF））的控制下，公开了将基因整合到甲基杆菌中，例如内切杆菌ATCC。还公开了感兴趣的基因的多重整合。针对Tn7附着物（attTn7）的独特和特异性的附着位点被鉴定为M.rerequens。

2. 发明申请

US20100221813A1 Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria 有权
标题翻译：调节甲基营养和甲烷营养细菌中的异源重组蛋白表达
公开(公告)号：US20100221813A1
公开(公告)日：2010-09-02
申请号：US11990779
申请日：2006-08-21
申请人： Carlos B. Miguez , Young-Jun Choi , Alaka Mullick , Bernard Massie
发明人： Carlos B. Miguez , Young-Jun Choi , Alaka Mullick , Bernard Massie
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/74 , C12P21/02
摘要： Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.
摘要翻译：用目的基因转化甲基营养型或甲烷营养型细菌（如甲基杆菌属），并通过与所关心的基因有效连接的丙烯酸阻遏蛋白和操纵子序列来调节基因的表达，并加入外部药剂。具体来说，来自恶臭假单胞菌的cymR阻遏物和cmt操纵子可以用于调节甲基营养型或甲烷营养型细菌中添加有机物的基因表达。

3. 发明授权

US08318455B2 Inducible/regulated gene expression system in E coli 有权
标题翻译：大肠杆菌诱导/调控基因表达系统
公开(公告)号：US08318455B2
公开(公告)日：2012-11-27
申请号：US12452232
申请日：2008-05-14
申请人： Young Jun Choi , Bernard Massie , Carlos B. Miguez
发明人： Young Jun Choi , Bernard Massie , Carlos B. Miguez
IPC分类号： C12P21/02
CPC分类号： C12N15/70
摘要： An expression system for transforming E coll with a nucleic acid molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E coll host cells may be transformed with the expression system. A method of producing a protein involves transforming an E coll host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will express the protein.
摘要翻译：用目的核酸分子转化E coll的表达系统具有与操作者的启动子可操作地连接的cmt操纵子的操纵子序列，以及可操作地连接到阻遏物启动子的cym操纵子的阻遏物序列。表达系统可以具有感兴趣的核酸分子，例如编码蛋白质的核酸分子。任何类型的E coll宿主细胞可以用表达系统进行转化。生产蛋白质的方法涉及用编码蛋白质的核酸分子的表达系统转化E coll宿主细胞，并且在核酸分子将表达蛋白质的条件下在培养基中培养宿主细胞。

4. 发明授权

US08216821B2 Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria 有权
标题翻译：调节甲基营养和甲烷营养细菌中的异源重组蛋白表达
公开(公告)号：US08216821B2
公开(公告)日：2012-07-10
申请号：US11990779
申请日：2006-08-21
申请人： Carlos B. Miguez , Young-Jun Choi , Alaka Mullick , Bernard Massie
发明人： Carlos B. Miguez , Young-Jun Choi , Alaka Mullick , Bernard Massie
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/74 , C12P21/02
摘要： Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.
摘要翻译：用目的基因转化甲基营养型或甲烷营养型细菌（如甲基杆菌属），并通过与所关心的基因有效连接的丙烯酸阻遏蛋白和操纵子序列来调节基因的表达，并加入外部药剂。具体来说，来自恶臭假单胞菌的cymR阻遏物和cmt操纵子可以用于调节甲基营养型或甲烷营养型细菌中添加有机物的基因表达。

5. 发明申请

US20100112636A1 INDUCIBLE/REGULATED GENE EXPRESSION SYSTEM IN E COLI 有权
标题翻译：可溶性/调控基因表达系统
公开(公告)号：US20100112636A1
公开(公告)日：2010-05-06
申请号：US12452232
申请日：2008-05-14
申请人： Young Jun Choi , Bernard Massie , Carlos B. Miguez
发明人： Young Jun Choi , Bernard Massie , Carlos B. Miguez
IPC分类号： C12P21/00 , C12N15/63 , C12N1/21
CPC分类号： C12N15/70
摘要： An expression system for transforming E. coli with a nucleic acid molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E. coli host cells may be transformed with the expression system. A method of producing a protein involves transforming an E. coli host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will express the protein.
摘要翻译：用感兴趣的核酸分子转化大肠杆菌的表达系统具有与操作者的启动子可操作地连接的cmt操纵子的操纵子序列，以及可操作地连接到阻遏物启动子的cym操纵子的阻遏物序列。表达系统可以具有感兴趣的核酸分子，例如编码蛋白质的核酸分子。可以用表达系统转化任何类型的大肠杆菌宿主细胞。生产蛋白质的方法涉及用具有编码蛋白质的核酸分子的表达系统转化大肠杆菌宿主细胞，并且在核酸分子将表达的条件下在培养基中培养宿主细胞蛋白。

6. 发明申请

US20080026005A1 Multicopy-integration of heterologous genes and expression in methylobacterium 有权
标题翻译：异源基因的多重整合和在甲基杆菌中的表达
公开(公告)号：US20080026005A1
公开(公告)日：2008-01-31
申请号：US11492822
申请日：2006-07-26
申请人： Carlos B. Miguez , Young-Jun Choi
发明人： Carlos B. Miguez , Young-Jun Choi
IPC分类号： A61K39/02 , C12N15/74 , C12N1/21
CPC分类号： C12N15/74
摘要： The integration of genes into methylobacterium, such as M. extorquens ATCC 55366 is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (PmxaF). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for M. extorquens.
摘要翻译：使用转座子系统（优选小型Tn7转座子系统）并在启动子如强甲醇脱氢酶启动子的控制下，公开了将基因整合到甲基杆菌中，例如外源半乳杆菌ATCC 55366（P < mxaF ）。还公开了感兴趣的基因的多重整合。针对Tn7附着物（attTn7）的独特和特异性的附着位点被鉴定为M.rerequens。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式