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1. 发明授权

US07091312B2 Biocidal protein 有权
公开(公告)号：US07091312B2
公开(公告)日：2006-08-15
申请号：US10409818
申请日：2003-04-08
申请人： Ching-San Chen , Kuan-Chung Chen , Cheng-Chun Kuan , Ching-Yu Lin
发明人： Ching-San Chen , Kuan-Chung Chen , Cheng-Chun Kuan , Ching-Yu Lin
IPC分类号： C07K14/415 , A01N37/18
CPC分类号： C07K14/415 , C12N15/8282 , C12N15/8286 , Y02A40/162
摘要： The invention relates to novel nucleic acid and protein sequences from the mung bean Vigna radiata. The nucleic acid sequence, isolated from a bruchid resistant mung bean line, encodes a thionin-like protein with insecticidal properties.

2. 发明授权

US06653463B1 Biocidal protein 失效
标题翻译：杀生物蛋白
公开(公告)号：US06653463B1
公开(公告)日：2003-11-25
申请号：US09686332
申请日：2000-10-11
申请人： Ching-San Chen , Kuan-Chung Chen , Cheng-Chun Kuan , Ching-Yu Lin
发明人： Ching-San Chen , Kuan-Chung Chen , Cheng-Chun Kuan , Ching-Yu Lin
IPC分类号： C12N1529
CPC分类号： C07K14/415 , C12N15/8282 , C12N15/8286 , Y02A40/162
摘要： The invention relates to novel nucleic acid and protein sequences from the mung bean Vigna radiata. The nucleic acid sequence, isolated from a bruchid resistant mung bean line, encodes a thionin-like protein with insecticidal properties.
摘要翻译：本发明涉及来自绿豆Vigna radiata的新型核酸和蛋白质序列。从Bruchid resistant绿豆系分离的核酸序列编码具有杀虫性质的硫素样蛋白质。

3. 发明申请

US20110166338A1 REAGENT, CHAOTROPIC AGENT, AND REAGENT KIT FOR AND APPLICATIONS OF ISOLATING NUCLEIC ACID BY USE OF MAGNETIC CELLULOSE MATERIAL 有权
标题翻译：通过使用磁性纤维素材料分离核酸的试剂，化学试剂和试剂盒和应用
公开(公告)号：US20110166338A1
公开(公告)日：2011-07-07
申请号：US12935681
申请日：2008-04-02
申请人： Pei-Shin Jiang , Yu-Ting Su , Kun-Chan Wu , Hui-Ju Cho , Wen-Hsun Kuo , Yuh-Jiuan Lin , Yi-Ling Li , Cheng-Chun Kuan
发明人： Pei-Shin Jiang , Yu-Ting Su , Kun-Chan Wu , Hui-Ju Cho , Wen-Hsun Kuo , Yuh-Jiuan Lin , Yi-Ling Li , Cheng-Chun Kuan
IPC分类号： C07H1/06 , C07H1/08 , H01F1/42
CPC分类号： C07H1/06 , C07H21/00 , C12N15/1013
摘要： Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.
摘要翻译：公开了一种离液剂; 包含离液剂和锂盐的试剂; 包含离液剂的试剂盒; 离液剂，试剂，试剂盒和通过使用磁性纤维素材料分离核酸的方法; 将核酸与磁性纤维素材料结合的方法; 分离核酸的方法; 以及纯化染色体DNA的方法。需要每种离液剂，试剂和试剂盒与至少一种固相含磁性纤维素的载体一起工作，以将核酸与非核酸物质分离。此外，各离液剂包括醇物质和用于将醇物质调节至适当浓度的底物溶液，从而促进样品中的核酸与磁性纤维素的结合。

4. 发明授权

US08163519B2 Fungal immunomodulatory protein (FIP) prepared by microorganisms and uses thereof 有权
标题翻译：通过微生物制备的真菌免疫调节蛋白（FIP）及其用途
公开(公告)号：US08163519B2
公开(公告)日：2012-04-24
申请号：US10572563
申请日：2004-09-14
申请人： Jiunn-Liang Ko , Yu-Lu Huang , Tzu-Chih Chen , Hsu-Wei Hung , Ho-Lung Jiang , Ching-Lung Hu , Cheng-Chun Kuan , Hsuan-Ju Thou
发明人： Jiunn-Liang Ko , Yu-Lu Huang , Tzu-Chih Chen , Hsu-Wei Hung , Ho-Lung Jiang , Ching-Lung Hu , Cheng-Chun Kuan , Hsuan-Ju Thou
IPC分类号： C12P21/04 , C12P21/06 , A61K39/00
CPC分类号： C07K14/37 , A61K36/064 , A61K38/00
摘要： The invention relates to an improved nucleic acid molecule encoding fungal immunomodulatory protein (FIP) that is better expressed in fungi, to vectors comprising the nucleic acid molecule, to hosts transformed with said vectors, to processes of expressing the protein of the invention in said transformed hosts, to the protein of the invention produced by said processes, to uses of said hosts comprising the protein of the invention and to a process of purifying FIP. The protein of the invention has wide immunomodulatory activity. Thus, the present invention further relates to uses of the protein of the invention in cosmetic or pharmaceutical compositions and to food or feed additives comprising the protein of the invention. Finally, the invention relates to the method of modulating immunological activities by orally administering FIP or proteins fused with FIP to a subject.
摘要翻译：本发明涉及一种编码真菌免疫调节蛋白（FIP）的改良的核酸分子，其在真菌中更好地表达，包含核酸分子的载体，用所述载体转化的宿主，在所述转化的表达本发明的蛋白质的过程中宿主，由所述方法产生的本发明的蛋白质，涉及包含本发明蛋白质的所述宿主的用途以及纯化FIP的方法。本发明的蛋白质具有广泛的免疫调节活性。因此，本发明还涉及本发明的蛋白质在化妆品或药物组合物中的用途，以及包含本发明蛋白质的食品或饲料添加剂的用途。最后，本发明涉及通过口服FIP或与FIP融合的蛋白质来调节免疫活性的方法。

5. 发明授权

US08513405B2 Reagent, chaotropic agent, and reagent kit for and applications of isolating nucleic acid by use of magnetic cellulose material 有权
标题翻译：试剂，离液剂和试剂盒，用于通过使用磁性纤维素材料分离核酸
公开(公告)号：US08513405B2
公开(公告)日：2013-08-20
申请号：US12935681
申请日：2008-04-02
申请人： Pei-Shin Jiang , Yu-Ting Su , Kun-Chan Wu , Hui-Ju Cho , Wen-Hsun Kuo , Yuh-Jiuan Lin , Yi-Ling Li , Cheng-Chun Kuan
发明人： Pei-Shin Jiang , Yu-Ting Su , Kun-Chan Wu , Hui-Ju Cho , Wen-Hsun Kuo , Yuh-Jiuan Lin , Yi-Ling Li , Cheng-Chun Kuan
IPC分类号： C07H21/02 , C07H21/00
CPC分类号： C07H1/06 , C07H21/00 , C12N15/1013
摘要： Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.
摘要翻译：公开了一种离液剂; 包含离液剂和锂盐的试剂; 包含离液剂的试剂盒; 离液剂，试剂，试剂盒和通过使用磁性纤维素材料分离核酸的方法; 将核酸与磁性纤维素材料结合的方法; 分离核酸的方法; 以及纯化染色体DNA的方法。需要每种离液剂，试剂和试剂盒与至少一种固相含磁性纤维素的载体一起工作，以将核酸与非核酸物质分离。此外，各离液剂包括醇物质和用于将醇物质调节至适当浓度的底物溶液，从而促进样品中的核酸与磁性纤维素的结合。

6. 发明授权

US08663582B2 Integral-type reaction cartridge 有权
标题翻译：积分式反应盒
公开(公告)号：US08663582B2
公开(公告)日：2014-03-04
申请号：US12846923
申请日：2010-07-30
申请人： Cheng-Chun Kuan , Wei-Sung Chen
发明人： Cheng-Chun Kuan , Wei-Sung Chen
IPC分类号： A61J1/06
CPC分类号： B01L3/5085 , B01L3/502 , B01L2300/0851 , G01N1/34 , G01N35/0098 , G01N2035/0436
摘要： An integral-type reaction cartridge includes a plurality of reaction containers and at least a separation container. The plural reaction containers and the separation container are integrally formed of plastic and lined along a first direction. The separation container has a first end, a second end opposing the first end, and a hollow accommodating space between the first and second ends, wherein the first end is an open end, the second end is a closed end, and the accommodating space tapers from the first end to the second end. The accommodating space has a first side and a second side along the first direction, as well as a third side and a fourth side along a second direction perpendicular to the first direction. Near the second end, at least one of the first side and the second side has a greater slope than the third side and the fourth side.
摘要翻译：整体型反应筒包括多个反应容器和至少一个分离容器。多个反应容器和分离容器由塑料一体地形成并沿着第一方向排列。分离容器具有第一端，与第一端相对的第二端和在第一和第二端之间的中空容纳空间，其中第一端是开口端，第二端是封闭端，容纳空间逐渐变细从第一端到第二端。容纳空间具有沿着第一方向的第一侧和第二侧，以及沿着与第一方向垂直的第二方向的第三侧和第四侧。在第二端附近，第一侧和第二侧中的至少一个具有比第三侧和第四侧更大的斜率。

7. 发明申请

US20110104028A1 INTEGRAL-TYPE REACTION CARTRIDGE 有权
标题翻译：集成式反应盒
公开(公告)号：US20110104028A1
公开(公告)日：2011-05-05
申请号：US12846923
申请日：2010-07-30
申请人： Cheng-Chun Kuan , Wei-Sung Chen
发明人： Cheng-Chun Kuan , Wei-Sung Chen
IPC分类号： B01J19/00
CPC分类号： B01L3/5085 , B01L3/502 , B01L2300/0851 , G01N1/34 , G01N35/0098 , G01N2035/0436
摘要： An integral-type reaction cartridge includes a plurality of reaction containers and at least a separation container. The plural reaction containers and the separation container are integrally formed of plastic and lined along a first direction. The separation container has a first end, a second end opposing the first end, and a hollow accommodating space between the first and second ends, wherein the first end is an open end, the second end is a closed end, and the accommodating space tapers from the first end to the second end. The accommodating space has a first side and a second side along the first direction, as well as a third side and a fourth side along a second direction perpendicular to the first direction. Near the second end, at least one of the first side and the second side has a greater slope than the third side and the fourth side.
摘要翻译：整体型反应筒包括多个反应容器和至少一个分离容器。多个反应容器和分离容器由塑料一体地形成并沿着第一方向排列。分离容器具有第一端，与第一端相对的第二端和在第一和第二端之间的中空容纳空间，其中第一端是开口端，第二端是封闭端，容纳空间逐渐变细从第一端到第二端。容纳空间具有沿着第一方向的第一侧和第二侧，以及沿着与第一方向垂直的第二方向的第三侧和第四侧。在第二端附近，第一侧和第二侧中的至少一个具有比第三侧和第四侧更大的斜率。

8. 发明申请

US20090042776A1 Fungal immunomodulatory protein (fip) prepared by microorganisms and uses thereof 有权
标题翻译：由微生物制备的真菌免疫调节蛋白（fip）及其用途
公开(公告)号：US20090042776A1
公开(公告)日：2009-02-12
申请号：US10572563
申请日：2004-09-14
申请人： Jiunn-Liang Ko , Yu-Lu Huang , Tzu-Chih Chen , Hsu-Wei Hung , Ho-Lung Jiang , Ching-Lung Hu , Cheng-Chun Kuan , Hsuan-Ju Thou
发明人： Jiunn-Liang Ko , Yu-Lu Huang , Tzu-Chih Chen , Hsu-Wei Hung , Ho-Lung Jiang , Ching-Lung Hu , Cheng-Chun Kuan , Hsuan-Ju Thou
IPC分类号： A61K38/00 , C12N15/11 , C12N1/19 , A61P31/00 , C12P21/04 , C07K14/00
CPC分类号： C07K14/37 , A61K36/064 , A61K38/00
摘要： The invention relates to an improved nucleic acid molecule encoding fungal immunomodulatory protein (FIP) that is better expressed in fungi, to vectors comprising the nucleic acid molecule, to hosts transformed with said vectors, to processes of expressing the protein of the invention in said transformed hosts, to the protein of the invention produced by said processes, to uses of said hosts comprising the protein of the invention and to a process of purifying FIP. The protein of the invention has wide immunomodulatory activity. Thus, the present invention further relates to uses of the protein of the invention in cosmetic or pharmaceutical compositions and to food or feed additives comprising the protein of the invention. Finally, the invention relates to the method of modulating immunological activities by orally administering FIP or proteins fused with FIP to a subject.
摘要翻译：本发明涉及一种编码真菌免疫调节蛋白（FIP）的改良的核酸分子，其在真菌中更好地表达，包含核酸分子的载体，用所述载体转化的宿主，在所述转化的表达本发明的蛋白质的过程中宿主，由所述方法产生的本发明的蛋白质，涉及包含本发明蛋白质的所述宿主的用途以及纯化FIP的方法。本发明的蛋白质具有广泛的免疫调节活性。因此，本发明还涉及本发明的蛋白质在化妆品或药物组合物中的用途，以及包含本发明蛋白质的食品或饲料添加剂的用途。最后，本发明涉及通过口服FIP或与FIP融合的蛋白质来调节免疫活性的方法。

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