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1. 发明授权

US09752154B2 High-throughput DNA fragment assembly 有权
公开(公告)号：US09752154B2
公开(公告)日：2017-09-05
申请号：US14416799
申请日：2013-07-23
申请人： DOW AGROSCIENCES LLC , Sandeep Kumar , Steven L. Evans , Manju Gupta
发明人： Sandeep Kumar , Steven L. Evans , Manju Gupta
IPC分类号： C12N15/68 , C12N15/82
CPC分类号： C12N15/8209 , C12N15/68 , C12N15/82
摘要： This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

2. 发明授权

US09688996B2 Use of a maize untranslated region for transgene expression in plants 有权
公开(公告)号：US09688996B2
公开(公告)日：2017-06-27
申请号：US14416811
申请日：2013-08-08
申请人： DOW AGROSCIENCES LLC , Sandeep Kumar , Manju Gupta , Diaa Alabed
发明人： Sandeep Kumar , Manju Gupta , Diaa Alabed
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8218
摘要： Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

3. 发明授权

US10358684B2 Targeted modification of malate dehydrogenase 有权
公开(公告)号：US10358684B2
公开(公告)日：2019-07-23
申请号：US15379152
申请日：2016-12-14
申请人： Dow AgroSciences LLC , Sangamo BioSciences, Inc.
发明人： Vipula Shukla , Manju Gupta , Fyodor Urnov , Dmitry Y. Guschin , Michiel Jan De Both , Paul Bundock , Lakshmi Sastry-Dent
IPC分类号： C12N15/82
摘要： Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

4. 发明授权

US10047367B2 Zea mays regulatory elements and uses thereof 有权
公开(公告)号：US10047367B2
公开(公告)日：2018-08-14
申请号：US14514833
申请日：2014-10-15
申请人： Dow AgroSciences LLC
发明人： Manju Gupta , Navin Elango , Karthik N. Muthuraman , Jeffrey Beringer , Sara Bennett , Huixia Wu , Shavell Gorman , Andrew Worden
IPC分类号： C12N15/113 , C12N15/82
摘要： Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5′-UTRs, and/or 3′-UTRs, isolated from Zea mays.

5. 发明申请

US20150067921A1 FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 有权
标题翻译： FAD3性能位置和相应目标位点特异性结合蛋白质可诱导目标突变
公开(公告)号：US20150067921A1
公开(公告)日：2015-03-05
申请号：US14019211
申请日：2013-09-05
申请人： Sangamo BioSciences, Inc. , Dow AgroSciences LLC
发明人： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , William Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
IPC分类号： C12N15/79 , C12N15/82 , C12N9/22 , C12N15/63
CPC分类号： C12N15/79 , C12N9/22 , C12N15/63 , C12N15/8241 , C12N15/8247
摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
摘要翻译：通过以位点方式切割细胞中FAD3基因的位置，在FAD3基因座中进行基因编辑或基因堆积的方法，以在FAD3基因中产生断裂，然后连接到断裂中，与核酸分子缔合公开了一种或多种感兴趣的特征。

6. 发明申请

US20130198898A1 METHOD AND CONSTRUCT FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER 有权
标题翻译：合成双向SCBV植物促进剂的方法和构建
公开(公告)号：US20130198898A1
公开(公告)日：2013-08-01
申请号：US13674712
申请日：2012-11-12
申请人： Dow AgroSciences LLC
发明人： Sandeep Kumar , Diaa Alabed , Sara Bennett , Manju Gupta , Susan Jayne , Terry R. Wright
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.
摘要翻译：提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

7. 发明申请

US20130104257A1 CONSTRUCT AND METHOD FOR EXPRESSING TRANSGENES USING A BRASSICA BIDIRECTIONAL CONSTITUTIVE PROMOTER 有权
标题翻译：使用BRASSICA双向构成促进剂表达转基因的构造和方法
公开(公告)号：US20130104257A1
公开(公告)日：2013-04-25
申请号：US13653602
申请日：2012-10-17
申请人： Dow AgroSciences LLC
发明人： Manju Gupta , Sean Michael Russell , Liu Yin Shen , Sivarama Reddy Chennareddy , Jyoti Rout , Stephen Novak
IPC分类号： C12N15/82
CPC分类号： C12N15/8278 , C07K2319/00 , C07K2319/81 , C12N9/1092 , C12N15/113 , C12N15/8243 , C12N15/8274 , C12N15/8275 , C12N2830/205 , C12Y205/01019
摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from Brassica napus or Brassica bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.
摘要翻译：提供了使用公开的来自甘蓝型油菜或芸苔属双侧组成型启动子（BBCP）的双向启动子在植物细胞和/或植物组织中表达多个基因的构建体和方法。提供的构建体包含至少一个与多个基因表达盒连接的双向启动子，其中每个基因表达盒包含至少一个转基因。在一些实施方案中，提供的构建体和方法允许在二至二十个之间表达基因。

8. 发明申请

US20130102002A1 METHOD TO DETERMINE ZYGOSITY OF THE FAD3 GENE IN CANOLA USING END-POINT TAQMAN® PCR 有权
标题翻译：使用终点TAQMAN®PCR确定CANOLA中FAD3基因的ZYGOSITY的方法
公开(公告)号：US20130102002A1
公开(公告)日：2013-04-25
申请号：US13656270
申请日：2012-10-19
申请人： Dow AgroSciences LLC
发明人： Lasantha Chandana Ubayasena , Zoe Ehlert , Manju Gupta , Chandra Shekara A. Channabasavaradhya
IPC分类号： G01N21/64
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.
摘要翻译：本发明的一部分涉及终点TaqMan PCR分析用于卡诺拉油菜中fad-3c基因的检测和高通量接合度分析。本发明进一步涉及使用野生型DNA作为用于确定接合性的参考。这些和其它相关方法可用于唯一地鉴定包含受试基因的卡诺拉系的接合度和品种。本发明还提供了用于确定来自卡诺拉植物或种子的样品的接合性的相关试剂盒。

9. 发明授权

US11053544B2 Corn event DAS-59122-7 and methods for detection thereof 有权
公开(公告)号：US11053544B2
公开(公告)日：2021-07-06
申请号：US15722697
申请日：2017-10-02
申请人： E. I. DuPont de Nemours and Company , Pioneer Hi-Bred International, Inc. , Dow Agrosciences LLC
发明人： James Wayne Bing , Robert F. Cressman, Jr. , Manju Gupta , Salim M. Hakimi , David Hondred , Todd L. Krone , Mary E. Hartnett Locke , Abigail K. Luckring , Sandra E. Meyer , Daniel Moellenbeck , Kenneth Edwin Narva , Paul D. Olson , Craig D. Sanders , Jimei Wang , Jian Zhang , Gan-Yuan Zhong
IPC分类号： C12Q1/6876 , C12Q1/6895 , C07H21/04 , C12N15/82 , C07K14/415
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

10. 发明申请

US20200087671A1 FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 审中-公开
公开(公告)号：US20200087671A1
公开(公告)日：2020-03-19
申请号：US16698077
申请日：2019-11-27
申请人： Dow AgroSciences LLC , Sangamo Therapeutics, Inc.
发明人： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , W. Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
IPC分类号： C12N15/79 , C12N15/82 , C12N15/63 , C12N9/22
摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

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