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    • 4. 发明授权
    • Method for preparing 2-pyrrolidone using a microorganism containing glutamate decarboxylase
    • 使用含有谷氨酸脱羧酶的微生物制备2-吡咯烷酮的方法
    • US08728775B2
    • 2014-05-20
    • US13876926
    • 2011-09-30
    • Dong Cheol ParkKi Kueon KangHyun Jung ParkSeo Hyoung KimJae Young Oh
    • Dong Cheol ParkKi Kueon KangHyun Jung ParkSeo Hyoung KimJae Young Oh
    • C12P17/10
    • C12P17/10C07D207/26C12N9/88C12P13/005C12P2203/00
    • The present invention relates to a method for preparing 2-pyrrolidone using biomass, comprising: a step (a) of culturing a microorganism which contains glutamate decarboxylase as a whole-cell catalyst in a culture medium containing glutamic acid or glutamate so as to prepare 4-amino butyric acid; a step (b) of filtering the 4-amino butyric acid from the culture medium in order to obtain the 4-amino butyric acid; and a step (c) of converting the 4-amino butyric acid into 2-pyrrolidone. The present invention provides a series of processes for preparing 2-pyrrolidone from glutamic acid or glutamate using biomass. According to the present invention, 4-amino butyric acid is prepared using a microorganism as a whole cell, and preferably, 4-amino butyric acid which has not undergone a complicated refining process such as a crystallization process is directly used to prepare 2-pyrrolidone at a high yield rate in an economically advantageous manner. According to the present invention, 2-pyrrolidone may be mass-produced at a high yield rate and low production costs. The method of the present invention simplifies processes for preparing 2-pyrrolidone, and thus is suitable for mass production at an industrial scale.
    • 本发明涉及使用生物质制备2-吡咯烷酮的方法,包括:(a)在含谷氨酸或谷氨酸的培养基中培养含谷氨酸脱羧酶作为全细胞催化剂的微生物,以制备4 - 氨基丁酸; 从培养基中过滤4-氨基丁酸以获得4-氨基丁酸的步骤(b); 和将4-氨基丁酸转化为2-吡咯烷酮的步骤(c)。 本发明提供了使用生物质从谷氨酸或谷氨酸制备2-吡咯烷酮的一系列方法。 根据本发明,使用微生物作为全细胞制备4-氨基丁酸,优选直接使用未经历复杂精制方法如结晶过程的4-氨基丁酸制备2-吡咯烷酮 以高收益率以经济上有利的方式。 根据本发明,2-吡咯烷酮可以高产率大批量生产,生产成本低。 本发明的方法简化了制备2-吡咯烷酮的方法,因此适用于工业规模的批量生产。
    • 7. 发明申请
    • METHOD FOR PREPARING 2-PYRROLIDONE USING BIOMASS
    • 使用生物质制备2-吡咯烷酮的方法
    • US20130203131A1
    • 2013-08-08
    • US13876926
    • 2011-09-30
    • Dong Cheol ParkKi Kueon KingHyun Jung ParkSeo Hyoung KimJae Young Oh
    • Dong Cheol ParkKi Kueon KingHyun Jung ParkSeo Hyoung KimJae Young Oh
    • C12P17/10
    • C12P17/10C07D207/26C12N9/88C12P13/005C12P2203/00
    • The present invention relates to a method for preparing 2-pyrrolidone using biomass, comprising: a step (a) of culturing a microorganism which contains glutamate decarboxylase as a whole-cell catalyst in a culture medium containing glutamic acid or glutamate so as to prepare 4-amino butyric acid; a step (b) of filtering the 4-amino butyric acid from the culture medium in order to obtain the 4-amino butyric acid; and a step (c) of converting the 4-amino butyric acid into 2-pyrrolidone. The present invention provides a series of processes for preparing 2-pyrrolidone from glutamic acid or glutamate using biomass. According to the present invention, 4-amino butyric acid is prepared using a microorganism as a whole cell, and preferably, 4-amino butyric acid which has not undergone a complicated refining process such as a crystallization process is directly used to prepare 2-pyrrolidone at a high yield rate in an economically advantageous manner. According to the present invention, 2-pyrrolidone may be mass-produced at a high yield rate and low production costs. The method of the present invention simplifies processes for preparing 2-pyrrolidone, and thus is suitable for mass production at an industrial scale.
    • 本发明涉及使用生物质制备2-吡咯烷酮的方法,包括:(a)在含谷氨酸或谷氨酸的培养基中培养含谷氨酸脱羧酶作为全细胞催化剂的微生物,以制备4 - 氨基丁酸; 从培养基中过滤4-氨基丁酸以获得4-氨基丁酸的步骤(b); 和将4-氨基丁酸转化为2-吡咯烷酮的步骤(c)。 本发明提供了使用生物质从谷氨酸或谷氨酸制备2-吡咯烷酮的一系列方法。 根据本发明,使用微生物作为全细胞制备4-氨基丁酸,优选直接使用未经历复杂精制方法如结晶过程的4-氨基丁酸制备2-吡咯烷酮 以高收益率以经济上有利的方式。 根据本发明,2-吡咯烷酮可以高产率大批量生产,生产成本低。 本发明的方法简化了制备2-吡咯烷酮的方法,因此适用于工业规模的批量生产。