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    • 1. 发明授权
    • Method of producing peptide
    • 生产肽的方法
    • US09346850B2
    • 2016-05-24
    • US14204074
    • 2014-03-11
    • KANEKA CORPORATION
    • Hiroshi MuraoKen-ichiro MorioMasaru Mitsuda
    • C07K1/06C07K1/02C07K5/065C07K5/072C07K5/083C07K5/103C07K1/14
    • C07K1/061C07K1/02C07K1/14C07K5/06078C07K5/06095C07K5/0806C07K5/0808C07K5/101
    • The present invention is related to a method of producing a peptide, characterized in contacting a reaction mixture with a base after a condensation reaction to hydrolyze while a basic condition is maintained until a ratio of a remaining unreacted active ester of an acid component is decreased to 1% or less in a liquid phase peptide synthesis method. According to the invention, a target peptide of high purity can be simply and efficiently produced by a continuous liquid phase synthesis method. Further, the present invention is related to a method of producing a peptide, characterized in using an amide-type solvent immiscible with water in a liquid phase peptide synthesis method. According to the invention, various peptides can be produced by the liquid phase synthesis method without being restricted by the amino acid sequence of the target peptide.
    • 本发明涉及一种生产肽的方法,其特征在于在缩合反应之后将反应混合物与碱反应以在碱性条件保持下进行水解,直到酸组分的剩余未反应的活性酯的比例降低至 在液相肽合成方法中为1%以下。 根据本发明,可以通过连续液相合成法简单有效地制备高纯度的靶肽。 此外,本发明涉及一种制备肽的方法,其特征在于在液相肽合成方法中使用与水不混溶的酰胺型溶剂。 根据本发明,可以通过液相合成法制备各种肽,而不受目标肽的氨基酸序列的限制。