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    • 7. 发明申请
    • Signal sequence trapping
    • 信号序列陷阱
    • US20080058214A1
    • 2008-03-06
    • US11341754
    • 2006-01-27
    • Fiona DuffnerReinhard WiltingKirk Schnorr
    • Fiona DuffnerReinhard WiltingKirk Schnorr
    • C40B20/00
    • C12N15/1034
    • The present invention allows the screening of previously established genebanks or libraries by proxy for genes encoding secreted, partially secreted, or cell surface-displayed polypeptides of industrial interest, such as enzymes, receptors, cytokines, peptide hormones etc. that would not likely have been isolated using conventional screening assays. A method for isolating genes encoding secreted, partially secreted, or cell surface displayed polypeptides from existing gene libraries is described in which the endogenous secretion signal sequences are detected using an in vitro polynucleotide insertion reaction where the inserted polynucleotide comprises a promoter-less and secretion signal-less secretion reporter.
    • 本发明允许通过代理方法筛选先前建立的基因库或文库,用于编码分泌的,部分分泌的或表面活性的细胞表达多肽,例如酶,受体,细胞因子,肽荷尔蒙等不可能已经被 使用常规筛选测定分离。 描述了从现有基因文库分离编码分泌的,部分分泌的或细胞表面展示的多肽的基因的方法,其中使用体外多核苷酸插入反应检测内源性分泌信号序列,其中插入的多核苷酸包含无启动子和分泌信号 无分泌记者。