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1. 发明申请

US20170051301A1 TRANSGENIC PLANTS WITH ENHANCED GROWTH CHARACTERISTICS 有权
标题翻译：具有增强生长特性的转基因植物
公开(公告)号：US20170051301A1
公开(公告)日：2017-02-23
申请号：US15256721
申请日：2016-09-05
申请人： Los Alamos National Security, LLC , University of Maine System Board of Trustees
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/82 , C12N9/00 , C12N9/10
CPC分类号： C12N15/8261 , C12N9/1096 , C12N9/93 , C12N15/8222 , C12N15/8267 , C12N15/827 , C12N15/8273 , C12Y206/01064 , C12Y603/01002 , Y02A40/146
摘要： The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.
摘要翻译：本发明涉及显着增强的生长速率，更大的种子和果实/荚果产量，更早和更有效的开花，更有效的氮利用，增加的对高盐条件的耐受性和增加的生物量产量的转基因植物。在一个实施方案中，提供了改造为过表达谷氨酰胺苯丙酮酸转氨酶（GPT）和谷氨酰胺合成酶（GS）的转基因植物。本发明的GPT + GS双转基因植物始终表现出增强的生长特性，其中T0代系显示生物量比野生型对应物的增加在50％至300％之间。由性交和/或自交产生的一代通常表现得更好，一些双转基因植物比野生型植物实现惊人的四倍生物量增加。

2. 发明申请

US20130239256A1 TRANSGENIC PLANTS WITH ENHANCED GROWTH CHARACTERISTICS 有权
标题翻译：具有增强生长特性的转基因植物
公开(公告)号：US20130239256A1
公开(公告)日：2013-09-12
申请号：US13714948
申请日：2012-12-14
申请人： LOS ALAMOS NATIONAL SECURITY, LLC , UNIVERSITY OF MAINE SYSTEM BOARD OF TRUSTEES
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N9/1096 , C12N9/93 , C12N15/8222 , C12N15/8267 , C12N15/827 , C12N15/8273 , C12Y206/01064 , C12Y603/01002 , Y02A40/146
摘要： The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.
摘要翻译：本发明涉及显着增强的生长速率，更大的种子和果实/荚果产量，更早和更有效的开花，更有效的氮利用，增加的对高盐条件的耐受性和增加的生物量产量的转基因植物。在一个实施方案中，提供了改造为过表达谷氨酰胺苯丙酮酸转氨酶（GPT）和谷氨酰胺合成酶（GS）的转基因植物。本发明的GPT + GS双转基因植物始终表现出增强的生长特性，其中T0代系显示生物量比野生型对应物的增加在50％至300％之间。由性交和/或自交产生的一代通常表现得更好，一些双转基因植物比野生型植物实现惊人的四倍生物量增加。

3. 发明授权

US09434956B2 Transgenic plants with enhanced growth characteristics 有权
标题翻译：具有增强生长特性的转基因植物
公开(公告)号：US09434956B2
公开(公告)日：2016-09-06
申请号：US13714948
申请日：2012-12-14
申请人： Los Alamos National Security, LLC , University of Maine System Board of Trustees
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/00 , C12N15/52 , A01H1/00 , C12N15/82 , C12N9/10 , A01H5/00
CPC分类号： C12N15/8261 , C12N9/1096 , C12N9/93 , C12N15/8222 , C12N15/8267 , C12N15/827 , C12N15/8273 , C12Y206/01064 , C12Y603/01002 , Y02A40/146
摘要： The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.
摘要翻译：本发明涉及显着增强的生长速率，更大的种子和果实/荚果产量，更早和更有效的开花，更有效的氮利用，增加的对高盐条件的耐受性和增加的生物量产量的转基因植物。在一个实施方案中，提供了改造为过表达谷氨酰胺苯丙酮酸转氨酶（GPT）和谷氨酰胺合成酶（GS）的转基因植物。本发明的GPT + GS双转基因植物始终表现出增强的生长特性，其中T0代系显示生物量比野生型对应物的增加在50％至300％之间。由性交和/或自交产生的一代通常表现得更好，一些双转基因植物比野生型植物实现惊人的四倍生物量增加。

4. 发明申请

US20130198908A1 NUCLEIC ACIDS ENCODING PLANT GLUTAMINE PHENYLPYRUVATE TRANSAMINASE (GPT) AND USES THEREOF 有权
标题翻译：核酸编码植物谷氨酰胺苯丙氨酸转移酶（GPT）及其用途
公开(公告)号：US20130198908A1
公开(公告)日：2013-08-01
申请号：US13734688
申请日：2013-01-04
申请人： Los Alamos National Security, LLC , University of Maine System Board of Trustees
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N9/10 , C12N15/82
CPC分类号： C12N9/1096 , C12N15/8261 , C12N15/8262 , C12N15/8269 , C12N15/827 , C12N15/8273 , C12Y206/01064 , Y02A40/146
摘要： Glutamine phenylpyruvate transaminase (GPT) proteins, nucleic acid molecules encoding GPT proteins, and uses thereof are disclosed. Provided herein are various GPT proteins and GPT gene coding sequences isolated from a number of plant species. As disclosed herein, GPT proteins share remarkable structural similarity within plant species, and are active in catalyzing the synthesis of 2-hydroxy-5-oxoproline (2-oxoglutaramate), a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism.
摘要翻译：公开了谷氨酰胺苯丙酮酸转氨酶（GPT）蛋白，编码GPT蛋白的核酸分子及其用途。本文提供了从许多植物物种分离的各种GPT蛋白和GPT基因编码序列。如本文所公开的，GPT蛋白在植物物种中具有显着的结构相似性，并且在催化2-羟基-5-氧代脯氨酸（2-氧代谷氨酸盐）的合成中是有活性的，其是调节大量涉及的基因的功能的强大的信号代谢物在光合作用装置中，碳固定和氮代谢。

5. 发明授权

US09862964B2 Transgenic plants with enhanced growth characteristics 有权
公开(公告)号：US09862964B2
公开(公告)日：2018-01-09
申请号：US15256721
申请日：2016-09-05
申请人： Los Alamos National Security, LLC , University of Maine System Board of Trustees
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： A01H1/00 , C12N15/82 , C12N9/10 , C12N9/00 , A01H5/00 , C12N15/00
CPC分类号： C12N15/8261 , C12N9/1096 , C12N9/93 , C12N15/8222 , C12N15/8267 , C12N15/827 , C12N15/8273 , C12Y206/01064 , C12Y603/01002 , Y02A40/146
摘要： The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.

6. 发明申请

US20170121728A1 PLANT GLUTAMINE PHENYLPYRUVATE TRANSAMINASE GENE AND TRANSGENIC PLANTS CARRYING SAME 审中-公开
公开(公告)号：US20170121728A1
公开(公告)日：2017-05-04
申请号：US15233879
申请日：2016-08-10
申请人： Los Alamos National Security, LLC , University of Maine System Board of Trustees
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/82 , A01H4/00
CPC分类号： C12N15/8261 , A01H4/008 , C12N9/1096 , C12N15/8262 , C12N15/827 , C12Y206/01064 , Y02A40/146
摘要： The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating growth-enhanced transgenic plants. In one embodiment, transgenic plants engineered to over-express glutamine phenylpyruvate transaminase (GPT) are provided.

7. 发明授权

US09296998B2 Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof 有权
标题翻译：编码植物谷氨酰胺苯丙酮酸转氨酶（GPT）的核酸及其用途
公开(公告)号：US09296998B2
公开(公告)日：2016-03-29
申请号：US13734688
申请日：2013-01-04
申请人： Los Alamos National Security, LLC , University of Maine System Board of Trustees
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/82 , C12N9/10
CPC分类号： C12N9/1096 , C12N15/8261 , C12N15/8262 , C12N15/8269 , C12N15/827 , C12N15/8273 , C12Y206/01064 , Y02A40/146
摘要： Glutamine phenylpyruvate transaminase (GPT) proteins, nucleic acid molecules encoding GPT proteins, and uses thereof are disclosed. Provided herein are various GPT proteins and GPT gene coding sequences isolated from a number of plant species. As disclosed herein, GPT proteins share remarkable structural similarity within plant species, and are active in catalyzing the synthesis of 2-hydroxy-5-oxoproline (2-oxoglutaramate), a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism.
摘要翻译：公开了谷氨酰胺苯丙酮酸转氨酶（GPT）蛋白，编码GPT蛋白的核酸分子及其用途。本文提供了从许多植物物种分离的各种GPT蛋白和GPT基因编码序列。如本文所公开的，GPT蛋白在植物物种中具有显着的结构相似性，并且在催化2-羟基-5-氧代脯氨酸（2-氧代谷氨酸盐）的合成中是有活性的，其是调节大量涉及的基因的功能的强大的信号代谢物在光合作用装置中，碳固定和氮代谢。

8. 发明申请

US20130232641A1 PLANT GLUTAMINE PHENYLPYRUVATE TRANSAMINASE GENE AND TRANSGENIC PLANTS CARRYING SAME 审中-公开
标题翻译：植物谷氨酰胺苯丙氨酸转移酶基因及其携带的转基因植物
公开(公告)号：US20130232641A1
公开(公告)日：2013-09-05
申请号：US13718214
申请日：2012-12-18
申请人： LOS ALAMOS NATIONAL SECURITY, LLC , UNIVERSITY OF MAINE SYSTEM BOARD OF TRUSTEES
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/82
CPC分类号： C12N15/8262 , C12N9/1096 , C12N15/8261 , Y02A40/146
摘要： The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating growth-enhanced transgenic plants. In one embodiment, transgenic plants engineered to over-express glutamine phenylpyruvate transaminase (GPT) are provided.
摘要翻译：本发明涉及表现出增强的生长速率，种子和果实产量以及总体生物量产量的转基因植物，以及用于产生生长增强的转基因植物的方法。在一个实施方案中，提供了改造为过表达谷氨酰胺苯丙酮酸转氨酶（GPT）的转基因植物。

9. 发明申请

US20160068854A1 INCREASING PLANT GROWTH BY MODULATING OMEGA-AMIDASE EXPRESSION IN PLANTS 审中-公开
标题翻译：通过调制植物中的OMEGA-AMIDASE表达来增加植物生长
公开(公告)号：US20160068854A1
公开(公告)日：2016-03-10
申请号：US14744478
申请日：2015-06-19
申请人： Los Alamos National Laboratory, LC/IP A 187 , UNIVERSITY OF MAINE SYSTEM BOARD OF TRUSTEES
发明人： Pat J. Unkefer , Penelope S. Anderson , Thomas J. Knight
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N9/80 , C12N15/8227 , C12N15/8262 , Y02A40/146
摘要： The present disclosure relates to compositions and methods for increasing the leaf-to-root ratio of the signal metabolite 2-oxoglutaramate and related proline molecules in plants by modulating levels of Ω-amidase to increase nitrogen use efficiency, resulting in enhanced growth, faster growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, increased tolerance to high salt conditions, and increased biomass yields.
摘要翻译：本公开内容涉及通过调节水平的和/或γ-谷氨酰胺酶以增加氮的使用效率来增加植物中信号代谢产物2-氧代谷氨酸和信号代谢产物的叶 - 根比的组合物和方法，从而增加生长，更快生长速度更快，种子和果实/荚果产量更高，开花时间越来越高，对高盐条件的耐受性越来越高，生物质产量提高。

10. 发明授权

US08802595B2 Use of prolines for improving growth and/or yield 有权
标题翻译：使用脯氨酸来改善生长和/或产量
公开(公告)号：US08802595B2
公开(公告)日：2014-08-12
申请号：US14047366
申请日：2013-10-07
申请人： Los Alamos National Security, LLC
发明人： Pat J. Unkefer , Rodolfo A. Martinez , Thomas J. Knight
IPC分类号： A01N43/46 , A61K9/00
CPC分类号： A01N43/36 , A01N37/38
摘要： The present invention describes a composition including a mixture of L- and D-pyroglutamate stereoisomers in a ratio of L to D of from about 80:20 to about 97:3 , and, a carrier medium for application of the L- and D-pyroglutamate stereoisomers to a target plant. The composition can also be used as a germination medium and may be incorporated into a seed coat for assisting in germination. The present invention further describes a method of increasing the agronomic performance of a target plant by treating a target plant with a composition including a mixture of L- and D-pyroglutamate stereoisomers in a ratio of L to D of from about 80:20 to about 97:3 and a carrier medium for said L- and D-pyroglutamate stereoisomers.
摘要翻译：本发明描述了一种组合物，其包含L至D的比例为约80:20至约97:3的L-和D-焦谷氨酸盐立体异构体的混合物，以及用于施用L-和D- 焦谷氨酸立体异构体。组合物也可以用作发芽培养基，并且可以并入种皮中以辅助发芽。本发明进一步描述了通过用包含L-和D-焦谷氨酸钠立体异构体的混合物的组合物处理目标植物，其比例为约80:20至约 97：3和用于所述L-和D-焦谷氨酸立体异构体的载体介质。

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