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1. 发明授权

US07667092B2 Processes and vectors for amplification or expression of nucleic acid sequences of interest in plants 有权
标题翻译：用于扩增或表达植物中感兴趣的核酸序列的方法和载体
公开(公告)号：US07667092B2
公开(公告)日：2010-02-23
申请号：US10476299
申请日：2002-03-27
申请人： Victor Klimyuk , Yuri Gleba , Mario Gils , Maxim Skulachev , Sylvestre Marillonet
发明人： Victor Klimyuk , Yuri Gleba , Mario Gils , Maxim Skulachev , Sylvestre Marillonet
IPC分类号： C12N15/82 , C12N15/83
CPC分类号： C07K14/005 , C12N15/8216 , C12N2770/40022
摘要： This invention discloses a biologically safe process of causing amplification and/or expression of one or more nucleic acid sequences of interest in a plant, plant tissue, plant cell or cell culture, characterized in that a plant cell is provided with at least two precursor vectors designed for undergoing processing by site-specific recombination in said cell, whereby due to said processing said plant cell is endowed with at least one replicon which provide(s) for said amplification and/or expression.
摘要翻译：本发明公开了在植物，植物组织，植物细胞或细胞培养物中引起目的的一种或多种核酸序列的扩增和/或表达的生物安全的方法，其特征在于向植物细胞提供至少两种前体载体设计用于通过所述细胞中的位点特异性重组进行处理，由此由于所述处理，所述植物细胞被赋予至少一个提供用于所述扩增和/或表达的复制子。

2. 发明授权

US09012729B2 Process of producing male sterile monocotyledonous plants 有权
标题翻译：生产雄性不育单子叶植物的方法
公开(公告)号：US09012729B2
公开(公告)日：2015-04-21
申请号：US12996364
申请日：2009-06-03
申请人： Mario Gils
发明人： Mario Gils
IPC分类号： C12N15/10 , C12N15/00 , C12N15/82 , C12N15/62
CPC分类号： C12N15/8213 , C12N15/62 , C12N15/8216 , C12N15/8265 , C12N15/8289
摘要： The present invention relates to a process of producing male sterile monocotyledonous plants by introducing into said plants fragments of a nucleotide sequence coding for a protein which provides for male sterility and reconstituting the complete protein which provides for male sterility by intein-mediated trans-splicing.
摘要翻译：本发明涉及通过向所述植物中引入编码提供雄性不育的蛋白质的核苷酸序列的片段，并通过内含肽介导的转接法重建提供雄性不育的完整蛋白质来生产雄性不育单子叶植物的方法。

3. 发明申请

US20110167513A1 PROCESS OF PRODUCING MALE STERILE MONOCOTYLEDONOUS PLANTS 有权
标题翻译：生产男性单倍体植物的方法
公开(公告)号：US20110167513A1
公开(公告)日：2011-07-07
申请号：US12996364
申请日：2009-06-03
申请人： Mario Gils
发明人： Mario Gils
IPC分类号： A01H1/02 , A01H1/06 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/62 , C12N15/8216 , C12N15/8265 , C12N15/8289
摘要： The present invention relates to a process of producing male sterile monocotyledonous plants by introducing into said plants fragments of a nucleotide sequence coding for a protein which provides for male sterility and reconstituting the complete protein which provides for male sterility by intein-mediated trans-splicing.
摘要翻译：本发明涉及通过向所述植物中引入编码提供雄性不育的蛋白质的核苷酸序列的片段，并通过内含肽介导的转拼连接重组提供雄性不育的完整蛋白质来生产雄性不育单子叶植物的方法。

4. 发明申请

US20080131933A1 Process of Producing a Plastid-Targeted Protein in Plant Cells 审中-公开
标题翻译：在植物细胞中产生质体靶向蛋白的过程
公开(公告)号：US20080131933A1
公开(公告)日：2008-06-05
申请号：US10556542
申请日：2004-05-13
申请人： Victor Klimyuk , Gregor Benning , Mario Gils , Anatoby Giritch , Yuri Gleba
发明人： Victor Klimyuk , Gregor Benning , Mario Gils , Anatoby Giritch , Yuri Gleba
IPC分类号： C12P21/04 , C07K7/00
CPC分类号： C07K14/56 , C07K14/61 , C07K2319/08 , C12N15/8221 , C12N15/8257
摘要： A process of producing a protein of interest in cells of a predetermined plant, comprising: introducing into said cells a vector encoding a fusion protein comprising from the N-terminus to the C-terminus (i) a transit peptide for targeting said fusion protein into plastids and, contiguous thereto, (ii) said protein of interest, wherein the C-terminal three amino acids X−3X−2X−1 of said transit peptide and the N-terminal amino acid Z of said protein of interest form a cleavage site X−3X−2X−1-Z for cleaving said fusion protein between X−1 and Z for releasing said protein of interest in plastids, whereby for a predetermined amino acid Z of said protein of interest an amino acid sequence X−3X−2X−1 is selected from the set of amino acid sequences X−3X−2X−1 naturally occurring contiguous to Z in plastid-targeted fusion proteins in plants, thereby forming said cleavage site.
摘要翻译：一种在预定植物的细胞中产生感兴趣的蛋白质的方法，包括：向所述细胞中引入编码包含N末端至C末端的融合蛋白的载体（i）用于靶向所述融合蛋白的转运肽质体，并且与其相邻，（ii）所述目的蛋白质，其中所述C末端三个氨基酸X-X 1，X 2，X 2，所述转运肽和所述目的蛋白质的N-末端氨基酸Z的SUP'形成切割位点X-S-X-X，用于将所述融合蛋白切割在X-1和Z之间，用于释放质体中所述目的蛋白质，由此对于目的蛋白质的预定氨基酸Z，氨基酸序列X < SUP> -3 从集合的氨基酸序列X 3 - 在植物中的质体靶向融合蛋白中与Z邻接天然存在的-2-2个<！ - SIPO

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