专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US11408890B2 Iterative expansion microscopy 有权
公开(公告)号：US11408890B2
公开(公告)日：2022-08-09
申请号：US15098799
申请日：2016-04-14
申请人： Massachusetts Institute of Technology
发明人： Edward Stuart Boyden , Jae-Byum Chang , Fei Chen , Paul Warren Tillberg
IPC分类号： G01N33/58 , G01N1/36 , G01N1/30 , G01N1/40
摘要： The present invention leverages the techniques for expansion microscopy (ExM) to provide improved high-throughput super-resolution whole-organ imaging methodology to image protein architectures over whole organs with nanoscale resolution by using high-throughput microscopes in combination with samples that have been iteratively expanded more than once, in a method referred to herein as “iterative expansion microscopy” (iExM). In the ExM method, biological samples of interest are permeated with a swellable material that results in the sample becoming embedded in the swellable material, and then the sample can be expanded isotropically in three dimensions The process of iteratively expanding the samples can be applied to samples that have been already expanded using ExM techniques one or more additional times to iteratively expand them such that, for example, a 5-fold expanded specimen can be expanded again 3- to 4-fold, resulting in as much as a 17- to 19-fold or more linear expansion.

2. 发明申请

US20130318483A1 STANDARDIZED TOPOGRAPHICAL ARRANGEMENTS FOR TEMPLATE REGIONS THAT ORIENT SELF-ASSEMBLY 有权
标题翻译：标准化地理位置对于自由组合模式区域的地形安排
公开(公告)号：US20130318483A1
公开(公告)日：2013-11-28
申请号：US13899936
申请日：2013-05-22
申请人： Massachusetts Institute of Technology
发明人： Jae-Byum Chang , Hong Kyoon Choi , Adam F. Hannon , Caroline A. Ross , Karl K. Berggren
IPC分类号： G06F17/50
CPC分类号： G06F17/50 , B81C1/00031 , B81C2201/0149 , B82Y40/00 , G03F7/0002
摘要： This disclosure relates generally to systems and methods of providing standardized topographical configurations for template regions. In one embodiment, a set of array arrangements is selected. Arrays of template structures are then formed on at least one substrate. Each of the arrays is arranged in accordance with an array arrangement in the set of array arrangements such that the arrays correspond surjectively onto the set of array arrangements. After the arrays are formed, a self-assembly material is provided on the arrays. Self-assembly patterns formed by self-assembling material as a result of the arrays may be empirically observed and used to map a set of self-assembly pattern arrangements surjectively onto the set of array arrangements. Using this mapping, a combination of the self-assembly pattern arrangements that match a target pattern arrangement can be used to select a combination of array arrangements from the set of array arrangements for a template region.
摘要翻译：本公开一般涉及为模板区域提供标准化的外形配置的系统和方法。在一个实施例中，选择一组阵列布置。然后在至少一个基底上形成模板结构的阵列。每个阵列根据阵列布置集合中的阵列布置被布置，使得阵列突出地对准阵列布置集合。在形成阵列之后，在阵列上提供自组装材料。可以凭经验观察自组装材料形成的自组装图案，并将其用于将一组自组装图案布置映射到阵列布置集合上。使用该映射，可以使用与目标图案布置匹配的自组装图案布置的组合来从模板区域的阵列布置集合中选择阵列布置的组合。

3. 发明申请

US20160305856A1 ITERATIVE EXPANSION MICROSCOPY 审中-公开
标题翻译：迭代扩展显微镜
公开(公告)号：US20160305856A1
公开(公告)日：2016-10-20
申请号：US15098799
申请日：2016-04-14
申请人： Massachusetts Institute of Technology
发明人： Edward Stuart Boyden , Jae-Byum Chang , Fei Chen , Paul Warren Tillberg
IPC分类号： G01N1/30 , G01N33/58
CPC分类号： G01N33/582 , G01N1/36 , G01N1/4044 , G01N2001/302 , G01N2001/307
摘要： The present invention leverages the techniques for expansion microscopy (ExM) to provide improved high-throughput super-resolution whole-organ imaging methodology to image protein architectures over whole organs with nanoscale resolution by using high-throughput microscopes in combination with samples that have been iteratively expanded more than once, in a method referred to herein as “iterative expansion microscopy” (iExM). In the ExM method, biological samples of interest are permeated with a swellable material that results in the sample becoming embedded in the swellable material, and then the sample can be expanded isotropically in three dimensions The process of iteratively expanding the samples can be applied to samples that have been already expanded using ExM techniques one or more additional times to iteratively expand them such that, for example, a 5-fold expanded specimen can be expanded again 3- to 4-fold, resulting in as much as a 17- to 19-fold or more linear expansion.
摘要翻译：本发明利用扩展显微镜技术（ExM）来提供改进的高通量超分辨率全器官成像方法，以通过使用高通量显微镜与已经迭代的样品组合来对纳米尺度分辨率的整个器官进行成像蛋白质结构在这里称为“迭代显微镜”（iExM）的方法中多次扩展。在ExM方法中，感兴趣的生物样品渗透出可膨胀的材料，导致样品变得嵌入可溶胀材料中，然后样品可以在三维方向上各向同性地膨胀。迭代扩展样品的过程可以应用于样品已经使用ExM技术已经扩展了一个或多个时间来迭代地扩展它们，使得例如5倍扩展的样本可以再次扩展3到4倍，导致17到19 倍或更多的线性膨胀。

4. 发明授权

US08966411B2 Standardized topographical arrangements for template regions that orient self-assembly 有权
标题翻译：用于定向自组装的模板区域的标准化地形布置
公开(公告)号：US08966411B2
公开(公告)日：2015-02-24
申请号：US13899936
申请日：2013-05-22
申请人： Massachusetts Institute of Technology
发明人： Jae-Byum Chang , Hong Kyoon Choi , Adam F. Hannon , Caroline A. Ross , Karl K. Berggren
IPC分类号： G06F17/50 , B81C1/00 , B82Y40/00
CPC分类号： G06F17/50 , B81C1/00031 , B81C2201/0149 , B82Y40/00 , G03F7/0002
摘要： This disclosure relates generally to systems and methods of providing standardized topographical configurations for template regions. In one embodiment, a set of array arrangements is selected. Arrays of template structures are then formed on at least one substrate. Each of the arrays is arranged in accordance with an array arrangement in the set of array arrangements such that the arrays correspond surjectively onto the set of array arrangements. After the arrays are formed, a self-assembly material is provided on the arrays. Self-assembly patterns formed by self-assembling material as a result of the arrays may be empirically observed and used to map a set of self-assembly pattern arrangements surjectively onto the set of array arrangements. Using this mapping, a combination of the self-assembly pattern arrangements that match a target pattern arrangement can be used to select a combination of array arrangements from the set of array arrangements for a template region.
摘要翻译：本公开一般涉及为模板区域提供标准化的外形配置的系统和方法。在一个实施例中，选择一组阵列布置。然后在至少一个基底上形成模板结构的阵列。每个阵列根据阵列布置集合中的阵列布置被布置，使得阵列突出地对准阵列布置组。在形成阵列之后，在阵列上提供自组装材料。可以凭经验观察自组装材料形成的自组装图案，并将其用于将一组自组装图案布置映射到阵列布置集合上。使用该映射，可以使用与目标图案布置匹配的自组装图案布置的组合来从模板区域的阵列布置集合中选择阵列布置的组合。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式