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    • 8. 发明申请
    • Method of selectively producing male or female sterile plants
    • 选择性生产雄性或雌性不育植物的方法
    • US20070174934A1
    • 2007-07-26
    • US10563779
    • 2004-06-09
    • Richard DaleTimothy HawkesPaul ThompsonRussell Viner
    • Richard DaleTimothy HawkesPaul ThompsonRussell Viner
    • A01H1/00C12N15/82C12N9/02C12N5/04A01N25/00
    • C12N9/0022C12N15/8287
    • A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male, or female reproductive structures and the non-phytotoxic substance is a D-alpha amino acid, or a peptide derivative of a non-protein D-alpha amino acid, characterised in that the enzyme is a mutant D-amino acid oxidase, obtainable from Rhodotorula gracilis, which oxidase comprises a lysine at position 58 rather than a phenylalanine in the wild type sequence.
    • 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,非植物毒性物质是D-α氨基酸或非蛋白D-α氨基酸的肽衍生物, 其特征在于该酶是从红景天红酵母获得的突变型D-氨基酸氧化酶,该氧化酶在58位包含赖氨酸而不是苯 野生型序列中的吡咯烷酮。