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    • 8. 发明授权
    • Method for identifying olfactory receptor included in one olfactory cell
    • 一种嗅觉细胞中鉴定嗅觉受体的方法
    • US08921048B2
    • 2014-12-30
    • US13784518
    • 2013-03-04
    • Panasonic Corporation
    • Masato Suzuki
    • C12Q1/68C12P19/34
    • C12Q1/6809C12Q1/6876C12Q1/6881
    • The present invention provides a novel method for identifying an olfactory receptor included in one olfactory cell. In the present invention, amplified is the cDNA derived from the mRNA of the one olfactory cell by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02. Subsequently, determined is whether or not a gene sequence of the amplified cDNA is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A. Finally, determined is that the olfactory receptor included in the one olfactory cell is the olfactory receptor corresponding to the one gene sequence which is identical to the gene sequence of the cDNA in the previous step, if the gene sequence of the cDNA is identical to the one gene sequence in the previous step.
    • 本发明提供了一种鉴定嗅觉细胞中包含的嗅觉受体的新方法。 在本发明中,通过使用由SEQ ID:01表示的正向引物和由SEQ ID:01表示的反向引物的PCR方法,扩增来自一个嗅觉细胞的mRNA的cDNA。随后,确定是否 扩增的cDNA的基因序列与包含在编码小鼠嗅觉受体A组中的嗅觉受体的基因序列中的一个基因序列相同。最后,确定一个嗅觉细胞中包含的嗅觉受体是对应于 如果cDNA的基因序列与前一步骤中的一个基因序列相同,则与前一步骤中的cDNA的基因序列相同的一个基因序列。
    • 9. 发明授权
    • Method for identifying olfactory receptor included in one olfactory cell
    • 一种嗅觉细胞中鉴定嗅觉受体的方法
    • US09051613B2
    • 2015-06-09
    • US13848158
    • 2013-03-21
    • Panasonic Corporation
    • Masato Suzuki
    • C12Q1/68
    • C12Q1/6881C12Q1/68C12Q1/6876
    • The present invention provides a novel method for identifying an olfactory receptor included in one olfactory cell. In the present invention, amplified is the cDNA derived from the mRNA of the one olfactory cell by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02. Subsequently, determined is whether or not a gene sequence of the amplified cDNA corresponds with one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A. Finally, determined is that the olfactory receptor included in the one olfactory cell is the olfactory receptor which corresponds to the one gene sequence which corresponds with the gene sequence of the cDNA in the previous step, if the gene sequence of the cDNA corresponds with the one gene sequence in the previous step.
    • 本发明提供了一种鉴定嗅觉细胞中包含的嗅觉受体的新方法。 在本发明中,通过使用由SEQ ID:01表示的正向引物和由SEQ ID:01表示的反向引物的PCR方法,扩增来自一个嗅觉细胞的mRNA的cDNA。随后,确定是否 扩增cDNA的基因序列对应于包含在编码小鼠嗅觉受体A组中的嗅觉受体的基因序列中的一个基因序列。最后,确定包含在一个嗅觉细胞中的嗅觉受体是嗅觉受体,其对应于 如果cDNA的基因序列与前一步骤中的一个基因序列相对应,则与前一步骤中的cDNA的基因序列相对应的一个基因序列。
    • 10. 发明授权
    • Method for identifying olfactory receptor included in one olfactory cell
    • 一种嗅觉细胞中鉴定嗅觉受体的方法
    • US08846318B2
    • 2014-09-30
    • US13848759
    • 2013-03-22
    • Panasonic Corporation
    • Masato Suzuki
    • C12Q1/68C07H21/04
    • C12Q1/6881C12Q1/6876
    • The present invention provides a novel method for identifying an olfactory receptor included in one olfactory cell. In the present invention, amplified is the cDNA derived from the mRNA of the one olfactory cell by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02. Subsequently, determined is whether or not a gene sequence of the amplified cDNA corresponds with one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A. Finally, determined is that the olfactory receptor included in the one olfactory cell is the olfactory receptor which corresponds to the one gene sequence which corresponds with the gene sequence of the cDNA in the previous step, if the gene sequence of the cDNA corresponds with the one gene sequence in the previous step.
    • 本发明提供了一种鉴定嗅觉细胞中包含的嗅觉受体的新方法。 在本发明中,通过使用由SEQ ID:01表示的正向引物和由SEQ ID:01表示的反向引物的PCR方法,扩增来自一个嗅觉细胞的mRNA的cDNA。随后,确定是否 扩增cDNA的基因序列对应于包含在编码小鼠嗅觉受体A组中的嗅觉受体的基因序列中的一个基因序列。最后,确定包含在一个嗅觉细胞中的嗅觉受体是嗅觉受体,其对应于 如果cDNA的基因序列与前一步骤中的一个基因序列相对应,则与前一步骤中的cDNA的基因序列相对应的一个基因序列。