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1. 发明授权

US07833713B2 Mitigation of Cot-1 DNA distortion in nucleic acid hybridization 有权
标题翻译：减轻核酸杂交中Cot-1 DNA的变形
公开(公告)号：US07833713B2
公开(公告)日：2010-11-16
申请号：US11561004
申请日：2006-11-17
申请人： Peter K. Rogan , Joan Knoll , Heather Newkirk
发明人： Peter K. Rogan , Joan Knoll , Heather Newkirk
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6832 , C12Q2525/151
摘要： A novel method of suppressing non-specific cross-hybridization between repetitive elements present in nucleic acid probes and corresponding repetitive elements in the target nucleic acid by using DNA synthesized to contain a plurality of repetitive elements while avoiding low and single copy sequences.
摘要翻译：通过使用合成含有多个重复元件的DNA同时避免低和单拷贝序列来抑制存在于核酸探针中的重复元件和靶核酸中的相应重复元件之间的非特异性交叉杂交的新方法。

2. 发明授权

US07811766B2 Genetic identification and validation of Echinacea species 失效
标题翻译：紫锥菊属的遗传鉴定和验证
公开(公告)号：US07811766B2
公开(公告)日：2010-10-12
申请号：US12058353
申请日：2008-03-28
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6895
摘要： A method for identification and validation of Echinacea is disclosed. Primers are designed based on information analysis of sequences from a large number of Echinacea species to amplify certain segments of genomic DNA to identify the species. Primers and methods are also disclosed to amplify other plant species that are frequently found in adulterated herbal samples of Echinacea.
摘要翻译：公开了一种用于鉴定和验证紫锥花的方法。基于来自大量松果菊物种的序列的信息分析来设计引物，以扩增某些基因组DNA片段以鉴定该物种。还公开了引物和方法以扩增在紫锥菊的掺假草药样品中经常发现的其它植物物种。

3. 发明申请

US20090081657A1 Genetic Identification And Validation Of Echinacea Species 失效
标题翻译：松果菊属的遗传鉴定和鉴定
公开(公告)号：US20090081657A1
公开(公告)日：2009-03-26
申请号：US12058353
申请日：2008-03-28
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6895
摘要： A method for identification and validation of Echinacea is disclosed. Primers are designed based on information analysis of sequences from a large number of Echinacea species to amplify certain segments of genomic DNA to identify the species. Primers and methods are also disclosed to amplify other plant species that are frequently found in adulterated herbal samples of Echinacea.
摘要翻译：公开了一种用于鉴定和验证紫锥花的方法。基于来自大量松果菊物种的序列的信息分析来设计引物，以扩增某些基因组DNA片段以鉴定该物种。还公开了引物和方法以扩增在紫锥菊的掺假草药样品中经常发现的其它植物物种。

4. 发明授权

US08532934B2 Rapid and comprehensive identification of prokaryotic organisms by metagenomic analysis 失效
标题翻译：通过宏基因组分析快速全面地鉴定原核生物
公开(公告)号：US08532934B2
公开(公告)日：2013-09-10
申请号：US13292545
申请日：2011-11-09
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： G01N33/48 , G01N31/00 , G06G7/48 , G06G7/58
CPC分类号： C12Q1/689
摘要： An improved method for rapid identification of microorganisms is disclosed, along with sequences of PCR primers optimized for this purpose. The primers are designed based on information analysis of sequences from a large number of organism to amplify certain segments of genomic DNA whose sequences are unique among different organisms. The PCR products are compared with a DNA sequence database to obtain the identity of the microorganisms. This approach provides an accurate and fast identification and taxonomic assignment of microbial species.
摘要翻译：公开了用于快速鉴定微生物的改进方法，以及为此目的优化的PCR引物序列。基于来自大量生物体的序列的信息分析来设计引物，以扩增其序列在不同生物体中是独特的基因组DNA的某些片段。将PCR产物与DNA序列数据库进行比较以获得微生物的身份。这种方法提供了对微生物物种的准确和快速的鉴定和分类分配。

5. 发明授权

US08209129B2 Ab initio generation of single copy genomic probes 有权
标题翻译：从头开始生成单拷贝基因组探针
公开(公告)号：US08209129B2
公开(公告)日：2012-06-26
申请号：US12794933
申请日：2010-06-07
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： G06F19/00 , C12N15/11
CPC分类号： G06F19/22 , G06F19/20
摘要： Single copy sequences suitable for use as DNA probes can be defined by computational analysis of genomic sequences. The present invention provides an ab initio method for identification of single copy sequences for use as probes which obviates the need to compare genomic sequences with existing catalogs of repetitive sequences. By dividing a target reference sequence into a series of shorter contiguous sequence windows and comparing these sequences with the reference genome sequence, one can identify single copy sequences in a genome. Probes can then be designed and produced from these single copy intervals.
摘要翻译：可以通过基因组序列的计算分析来定义适合用作DNA探针的单拷贝序列。本发明提供了用于鉴定用作探针的单拷贝序列的从头法，其不需要将基因组序列与现有的重复序列目录进行比较。通过将目标参考序列划分成一系列较短的连续序列窗口并将这些序列与参考基因组序列进行比较，可以鉴定基因组中的单拷贝序列。然后可以从这些单个复印间隔设计和生产探针。

6. 发明授权

US5849492A Method for rapid identification of prokaryotic and eukaryotic organisms 失效
标题翻译：快速鉴定原核和真核生物的方法
公开(公告)号：US5849492A
公开(公告)日：1998-12-15
申请号：US744722
申请日：1996-10-29
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6876 , C07H21/00 , C12Q1/6811 , C12Q1/689 , C12Q1/6895
摘要： A method is described for retrieval of phylogenetically informative DNA sequences which comprise searching for a highly divergent segment of genomic DNA surrounded by two highly conserved segments, designing the universal primers for PCR amplification of the highly divergent region, and amplifying the genomic DNA by PCR technique using universal primers.
摘要翻译：描述了一种用于检索系统发育性信息DNA序列的方法，其包括搜索由两个高度保守区段包围的基因组DNA的高度不同部分，设计用于高度发散区域的PCR扩增的通用引物，以及通过PCR技术扩增基因组DNA 使用通用引物。

7. 发明授权

US08527207B2 Accurate identification of organisms based on individual information content 失效
标题翻译：基于个人信息内容准确识别生物
公开(公告)号：US08527207B2
公开(公告)日：2013-09-03
申请号：US12152610
申请日：2008-05-15
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： G01N33/48 , G01N31/00 , G06G7/48 , G06G7/58
CPC分类号： C12Q1/689 , C12Q1/701 , G06F19/14 , G06F19/20 , G06F19/22 , G06F19/24
摘要： An improved method for specific identification of any organisms by DNA hybridization or amplification is disclosed. Oligonucleotides are designed based on information analysis of sequences from a large number of related species. Oligonucleotide sequences that have the maximal specificity to certain nucleic acids from a particular species (or set of species) or type strain are selected for hybridization or amplification using DNA from the target organism. The presence or absence of a PCR or hybridization product may be used to identify the target organism. The resulting PCR products may also be compared with a DNA sequence database to obtain the identity of the organisms. The methods may prove useful in areas where rapid and accurate identification of an organism is desirable, such as in a hospital where identification of infectious agents may be critical, in the ethanol or beer industry where certain bacteria may be detrimental to the manufacturing process, or in the porcine industry where identification of different type strains of the porcine reproductive and respiratory syndrome virus (PRRV) is important for disease prevention.
摘要翻译：公开了通过DNA杂交或扩增来特异性鉴定任何生物体的改进方法。基于来自大量相关物种的序列的信息分析来设计寡核苷酸。选择具有来自特定物种（或一组物种）或类型菌株的某些核酸的最大特异性的寡核苷酸序列用于来自靶生物体的DNA进行杂交或扩增。可以使用PCR或杂交产物的存在或不存在来鉴定靶生物体。所得到的PCR产物也可以与DNA序列数据库进行比较以获得生物体的身份。在某些细菌可能对制造过程有害的乙醇或啤酒工业中，这些方法可能证明在需要快速准确地识别生物体的领域中是有用的，例如在感染因子鉴别可能是关键的医院中，或在猪繁殖与呼吸综合征病毒（PRRV）不同型号的鉴定中猪疫苗对疾病预防具有重要意义。

8. 发明申请

US20080286797A1 Accurate identification of organisms based on individual information content 失效
标题翻译：基于个人信息内容准确识别生物
公开(公告)号：US20080286797A1
公开(公告)日：2008-11-20
申请号：US12152610
申请日：2008-05-15
申请人： Peter K. Rogan
发明人： Peter K. Rogan
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q1/701 , G06F19/14 , G06F19/20 , G06F19/22 , G06F19/24
摘要： An improved method for specific identification of any organisms by DNA hybridization or amplification is disclosed. Oligonucleotides are designed based on information analysis of sequences from a large number of related species. Oligonucleotide sequences that have the maximal specificity to certain nucleic acids from a particular species (or set of species) or type strain are selected for hybridization or amplification using DNA from the target organism. The presence or absence of a PCR or hybridization product may be used to identify the target organism. The resulting PCR products may also be compared with a DNA sequence database to obtain the identity of the organisms. The methods may prove useful in areas where rapid and accurate identification of an organism is desirable, such as in a hospital where identification of infectious agents may be critical, in the ethanol or beer industry where certain bacteria may be detrimental to the manufacturing process, or in the porcine industry where identification of different type strains of the porcine reproductive and respiratory syndrome virus (PRRV) is important for disease prevention.
摘要翻译：公开了通过DNA杂交或扩增来特异性鉴定任何生物体的改进方法。基于来自大量相关物种的序列的信息分析来设计寡核苷酸。选择具有来自特定物种（或一组物种）或类型菌株的某些核酸的最大特异性的寡核苷酸序列用于来自靶生物体的DNA进行杂交或扩增。可以使用PCR或杂交产物的存在或不存在来鉴定靶生物体。所得到的PCR产物也可以与DNA序列数据库进行比较以获得生物体的身份。在某些细菌可能对制造过程有害的乙醇或啤酒工业中，这些方法可能证明在需要快速准确地识别生物体的领域中是有用的，例如在感染因子鉴别可能是关键的医院中，或在猪繁殖与呼吸综合征病毒（PRRV）不同型号的鉴定中猪疫苗对疾病预防具有重要意义。

9. 发明授权

US06828097B1 Single copy genomic hybridization probes and method of generating same 失效
标题翻译：单拷贝基因组杂交探针及其产生方法
公开(公告)号：US06828097B1
公开(公告)日：2004-12-07
申请号：US09573080
申请日：2000-05-16
申请人： Joan H. M. Knoll , Peter K. Rogan
发明人： Joan H. M. Knoll , Peter K. Rogan
IPC分类号： C12Q168
CPC分类号： C12Q1/6876 , C12Q1/6841 , C12Q2600/156
摘要： Nucleic acid (e.g., DNA) hybridization probes are described which comprise a labeled, single copy nucleic acid which hybridizes to a deduced single copy sequence interval in target nucleic acid of known sequence. The probes, which are essentially free of repetitive sequences, can be used in hybridization analyses without adding repetitive sequence-blocking nucleic acids. This allows rapid and accurate detection of chromosomal abnormalities. The probes are preferably designed by first determining the sequence of at least one single copy interval in a target nucleic acid sequence, and developing corresponding hybridization probes which hybridize to at least a part of the deduced single copy sequence. In practice, the sequences of the target and of known genomic repetitive sequence representatives are compared in order to deduce locations of the single copy sequence intervals. The single copy probes can be developed by any variety of methods, such as PCR amplification, restriction or exonuclease digestion of purified genomic fragments, or direct synthesis of DNA sequences. This is followed by labeling of the probes and hybridization to a target sequence.
摘要翻译：描述了核酸（例如DNA）杂交探针，其包含与已知序列的靶核酸中推断的单拷贝序列间隔杂交的标记的单拷贝核酸。基本上不含重复序列的探针可用于杂交分析，而不添加重复的序列阻断核酸。这样可以快速准确地检测染色体异常。探针优选通过首先确定靶核酸序列中至少一个单拷贝间隔的序列，并开发与所推导的单拷贝序列的至少一部分杂交的相应杂交探针来设计。在实践中，比较了靶和已知基因组重复序列代表的序列，以推导出单拷贝序列间隔的位置。单拷贝探针可以通过各种方法开发，如PCR扩增，纯化的基因组片段的限制或外切核酸酶消化，或直接合成DNA序列。然后将探针标记并与靶序列杂交。

10. 发明授权

US5867402A Computational analysis of nucleic acid information defines binding sites 失效
标题翻译：核酸信息的计算分析定义了结合位点
公开(公告)号：US5867402A
公开(公告)日：1999-02-02
申请号：US494115
申请日：1995-06-23
申请人： Thomas D. Schneider , Peter K. Rogan
发明人： Thomas D. Schneider , Peter K. Rogan
IPC分类号： C12Q1/68 , G06F19/18 , G06F19/22 , G06T7/00 , G06F17/16
CPC分类号： G06T7/0012 , C12Q1/68 , C12Q1/6827 , G06F19/22 , G06F19/18
摘要： In accordance with the present invention, binding sites are defined based upon the individual information content of a particular site of interest. Substitutions within the binding site sequences can be analyzed to determine whether the substitution will cause a deleterious mutation or a benign polymorphism. In addition, new binding sites can be identified using individual information content. Further a computer system is described for determining and displaying individual information content of a binding site sequence.
摘要翻译：根据本发明，基于感兴趣的特定位点的个体信息内容来定义结合位点。可以分析结合位点序列内的取代以确定取代是否会引起有害突变或良性多态性。此外，可以使用个人信息内容来识别新的结合位点。此外，描述了用于确定和显示绑定站点序列的个体信息内容的计算机系统。

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