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1. 发明授权

US11618023B2 Microfluidic device and a method for provision of emulsion droplets 有权
公开(公告)号：US11618023B2
公开(公告)日：2023-04-04
申请号：US16770138
申请日：2018-12-04
申请人： SAMPLIX APS
发明人： Thomas Kvist , Solène Cherre , Marie Just Mikkelsen
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011
摘要： The present invention relates to a microfluidic device and method for providing emulsion droplets. The device comprising: a microfluidic section comprising one or more microfluidic units; and a well section comprising one or more groups of wells comprising one group of wells for each microfluidic unit; the well section and the microfluidic section forming a fixedly connected unit such that each group of wells forms a fixedly connected unit with a respective corresponding microfluidic unit, each microfluidic unit comprising a fluid conduit network comprising: a plurality of supply conduits comprising a secondary supply conduit and a primary supply conduit comprising a capillary structure having a volume of at least 2 μL; a transfer conduit; and a first fluid junction providing fluid communication between the primary supply conduit, the secondary supply conduit, and the transfer conduit; each group of wells comprising a plurality of wells comprising a collection well and one or more supply wells comprising a primary supply well, the collection well being in fluid communication with the transfer conduit of the corresponding microfluidic unit, the primary supply well being in fluid communication with the primary supply conduit and the secondary supply conduit of the corresponding microfluidic unit.

2. 发明授权

US11078507B2 Probability-directed isolation of nucleotide sequences (PINS) 有权
公开(公告)号：US11078507B2
公开(公告)日：2021-08-03
申请号：US16238753
申请日：2019-01-03
申请人： Samplix ApS
发明人： Thomas Kvist , Marie Just Mikkelsen
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/6848 , C12Q1/686
摘要： The present invention pertains to an in vitro method in which the frequency of the targeted nucleotide sequence containing the DNA fragment of interest is increased stepwise, by several rounds of 1) dilution of a sample containing the DNA fragment of interest into several replicates (separation), 2) randomly amplifying DNA in the replicates (concentration), 3) detecting the DNA fragment of interest in at least one of the diluted and amplified replicates (selection) and repeating steps 1) through 3) until the DNA fragment of interest can be sequenced by standard sequencing techniques.

3. 发明申请

US20220105515A1 A MICROFLUIDIC DEVICE AND A METHOD FOR PROVISION OF EMULSION DROPLETS 有权
公开(公告)号：US20220105515A1
公开(公告)日：2022-04-07
申请号：US17426554
申请日：2020-01-31
申请人： SAMPLIX APS
发明人： Thomas Kvist , Soléne Cherre , Marie Just Mikkelsen
IPC分类号： B01L3/00
摘要： A microfluidic device (100) comprises an emulsification section (101) comprising one or more emulsification units (170); and a container section (102) comprising one or more groups of containers comprising one group of containers for each emulsification unit; each emulsification unit (170) comprising a fluid conduit network (135) comprising: a plurality of supply conduits comprising a primary supply conduit (103) and a secondary supply conduit (106); a transfer conduit (112); and a first fluid junction (120) providing fluid communication between the primary supply conduit (103), the secondary supply conduit (106), and the transfer conduit (112); each group of containers (103) comprising a plurality of containers comprising an intermediate chamber (174), a collection container (134), and one or more supply containers (131) comprising a secondary supply container, the secondary supply container (131) defining a secondary supply cavity, the secondary supply container (131) comprising a secondary orifice (177) extending from the secondary supply cavity and a primary orifice (176) extending from the secondary supply cavity, the collection container (134) being in fluid communication with the transfer conduit (112) of the corresponding emulsification unit (170) via a collection orifice of the collection container (134), the secondary supply container (131) being in fluid communication with the secondary supply conduit (106) of the corresponding emulsification unit (170) via the secondary orifice (177), the secondary supply container (131) being in fluid communication with the intermediate chamber (174) of the same group of containers (103) via the primary orifice (176), the intermediate chamber (174) being in fluid communication with the first fluid junction (120) of the corresponding emulsification unit (170) via the primary supply conduit (103) of the corresponding emulsification unit (170). Furthermore a method of manufacturing said device and a method for providing emulsion droplets using such a microfluidic device.

4. 发明授权

US10738354B2 Targeted enrichment of long nucleotide sequences using microfluidic partitioning 有权
公开(公告)号：US10738354B2
公开(公告)日：2020-08-11
申请号：US15739541
申请日：2016-06-24
申请人： Samplix ApS
发明人： Marie Just Mikkelsen , Thomas Kvist , Esben Bjørn Madsen
IPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/6806 , C12Q1/686
摘要： The invention relates to a method for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element. The methods include, inter alia, steps of providing a liquid sample of mixed DNA molecules comprising one or more specific target DNA molecules, formation of a multiple of liquid droplets, specific detection of droplets containing at least one of the target DNA molecules, physically selecting droplets containing at least one of the target DNA molecules, and general amplification of the DNA molecules in the coalesced selected droplets.

5. 发明授权

US11779923B2 Microfluidic device and a method for provision of double emulsion droplets 有权
公开(公告)号：US11779923B2
公开(公告)日：2023-10-10
申请号：US16770135
申请日：2018-12-04
申请人： SAMPLIX APS
发明人： Thomas Kvist , Esben Bjørn Madsen , Solène Cherre , Marie Just Mikkelsen
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/301 , B01F33/30 , B01F101/23
CPC分类号： B01L3/502784 , B01F23/4105 , B01F33/3011 , B01F33/3017 , B01L3/502707 , B01L3/502715 , B01F23/4143 , B01F23/4144 , B01F2101/23 , B01L2200/028 , B01L2200/06 , B01L2200/0673 , B01L2200/12 , B01L2200/141 , B01L2300/0681 , B01L2300/0867
摘要： The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.

6. 发明授权

US11572584B2 Targeted enrichment of long nucleotide sequences using microfluidic partitioning 有权
公开(公告)号：US11572584B2
公开(公告)日：2023-02-07
申请号：US16921103
申请日：2020-07-06
申请人： Samplix ApS
发明人： Marie Just Mikkelsen , Thomas Kvist , Esben Bjørn Madsen
IPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/6806 , C12Q1/686
摘要： The present disclosure relates to methods for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element. The methods herein comprise the steps of forming an emulsion of multiple liquid droplets from a DNA fragment containing liquid sample, specific detection of droplets containing at least one target DNA molecule, physically selecting droplets containing at least one target DNA molecule, and performing a general amplification of the DNA molecules containing at least one target DNA molecules.

7. 发明授权

US10214759B2 Probability-directed isolation of nucleotide sequences (PINS) 有权
公开(公告)号：US10214759B2
公开(公告)日：2019-02-26
申请号：US14653795
申请日：2013-12-20
申请人： Samplix ApS
发明人： Thomas Kvist , Marie Just Mikkelsen
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/6848 , C12Q1/686
摘要： The present invention pertains to an in vitro method in which the frequency of the targeted nucleotide sequence containing the DNA fragment of interest is increased stepwise, by several rounds of 1) dilution of a sample containing the DNA fragment of interest into several replicates (separation), 2) randomly amplifying DNA in the replicates (concentration), 3) detecting the DNA fragment of interest in at least one of the diluted and amplified replicates (selection) and repeating steps 1) through 3) until the DNA fragment of interest can be sequenced by standard sequencing techniques.

8. 发明申请

US20220105516A1 A MICROFLUIDIC DEVICE AND A METHOD FOR PROVISION OF DOUBLE EMULSION DROPLETS 有权
公开(公告)号：US20220105516A1
公开(公告)日：2022-04-07
申请号：US17426549
申请日：2020-01-31
申请人： SAMPLIX APS
发明人： Thomas Kvist , Esben Bjørn Madsen , Soléne Cherre , Marie Just Mikkeisen
IPC分类号： B01L3/00
摘要： A microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets.

9. 发明申请

US20200332353A1 TARGETED ENRICHMENT OF LONG NUCLEOTIDE SEQUENCES USING MICROFLUIDIC PARTITIONING 审中-公开
公开(公告)号：US20200332353A1
公开(公告)日：2020-10-22
申请号：US16921103
申请日：2020-07-06
申请人： Samplix ApS
发明人： Marie Just Mikkelsen , Thomas Kvist , Esben Bjørn Madsen
IPC分类号： C12Q1/6848 , C12Q1/6806 , C12Q1/686
摘要： The invention relates to a method for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element.

10. 发明授权

US10704080B2 Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS) 有权
公开(公告)号：US10704080B2
公开(公告)日：2020-07-07
申请号：US15317050
申请日：2015-06-11
申请人： Samplix ApS
发明人： Marie Just Mikkelsen , Thomas Kvist
IPC分类号： C12Q1/68 , C07H21/00 , C12Q1/6806 , C12Q1/6848 , C12Q1/686
摘要： The present invention pertains to an in vitro method in which a targeted DNA molecule containing a DNA sequence of interest is enriched by a) general amplification of DNA molecules in a multiple of droplets each containing less than 0.5 target DNA molecule on average (404), b) specific detection of the target DNA molecule in each of the droplets (405), and c) physically selecting droplets containing target DNA molecules (406).

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