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    • 3. 发明授权
    • Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
    • 使用纵向置换的纳米级电极用于在流体通道中对生物分子和其他分析物进行电压检测
    • US08882980B2
    • 2014-11-11
    • US12553667
    • 2009-09-03
    • Xinsheng LingBarrett BreadyJohn S. OliverMaryam JouziLeo Petrossian
    • Xinsheng LingBarrett BreadyJohn S. OliverMaryam JouziLeo Petrossian
    • G01N27/447G01N27/26G01N33/487B82Y15/00C07K1/26B01L3/00G01N27/327
    • G01N33/48721B01L3/502761B82Y15/00G01N27/3278Y10S977/957
    • Devices and methods for detecting an analyte are provided. Devices for voltage sensing of analytes may comprise a fluidic channel defined in a substrate, a pair of sensing electrodes disposed in a fluidic channel for sensing voltage therein, and a pair of electromotive electrodes for applying potential along the fluidic channel. The pair of sensing electrodes may include a first and second sensing electrode disposed at two discrete locations along the length of the fluidic channel and the pair of electromotive electrodes may be disposed at a first end and a second end of the fluidic channel. The fluidic channel may include a nanochannel or a microchannel. Methods for detecting an analyte may include the steps of disposing the analyte in a fluidic channel; applying a potential along the fluidic channel to generate an electrophoretic force therein such that the analyte is translocated from a first end of the fluidic channel to a second end of the fluidic channel; and measuring a voltage signal between a pair of sensing electrodes disposed in the fluidic channel as the analyte moves past the sensing electrodes.
    • 提供了用于检测分析物的装置和方法。 用于分析物的电压感测的装置可以包括限定在衬底中的流体通道,设置在用于感测电压的流体通道中的一对感测电极以及用于沿着流体通道施加电位的一对电动电极。 一对感测电极可以包括设置在沿着流体通道的长度的两个离散位置处的第一和第二感测电极,并且该对电动电极可以设置在流体通道的第一端和第二端。 流体通道可以包括纳米通道或微通道。 用于检测分析物的方法可以包括将分析物置于流体通道中的步骤; 沿着流体通道施加电位以在其中产生电泳力,使得分析物从流体通道的第一端转位到流体通道的第二端; 以及当分析物移动通过感测电极时,测量设置在流体通道中的一对感测电极之间的电压信号。
    • 4. 发明申请
    • USE OF LONGITUDINALLY DISPLACED NANOSCALE ELECTRODES FOR VOLTAGE SENSING OF BIOMOLECULES AND OTHER ANALYTES IN FLUIDIC CHANNELS
    • 用于电流传感生物分子和流体通道中其他分析物的长距离放置的纳米电极的应用
    • US20100096268A1
    • 2010-04-22
    • US12553667
    • 2009-09-03
    • Xinsheng LingBarrett BreadyJohn S. OliverMaryam JouziLeo Petrossian
    • Xinsheng LingBarrett BreadyJohn S. OliverMaryam JouziLeo Petrossian
    • G01N27/447G01N27/26
    • G01N33/48721B01L3/502761B82Y15/00G01N27/3278Y10S977/957
    • Devices and methods for detecting an analyte are provided. Devices for voltage sensing of analytes may comprise a fluidic channel defined in a substrate, a pair of sensing electrodes disposed in a fluidic channel for sensing voltage therein, and a pair of electromotive electrodes for applying potential along the fluidic channel. The pair of sensing electrodes may include a first and second sensing electrode disposed at two discrete locations along the length of the fluidic channel and the pair of electromotive electrodes may be disposed at a first end and a second end of the fluidic channel. The fluidic channel may include a nanochannel or a microchannel. Methods for detecting an analyte may include the steps of disposing the analyte in a fluidic channel; applying a potential along the fluidic channel to generate an electrophoretic force therein such that the analyte is translocated from a first end of the fluidic channel to a second end of the fluidic channel; and measuring a voltage signal between a pair of sensing electrodes disposed in the fluidic channel as the analyte moves past the sensing electrodes.
    • 提供了用于检测分析物的装置和方法。 用于分析物的电压感测的装置可以包括限定在衬底中的流体通道,设置在用于感测电压的流体通道中的一对感测电极以及用于沿着流体通道施加电位的一对电动电极。 一对感测电极可以包括设置在沿着流体通道的长度的两个离散位置处的第一和第二感测电极,并且该对电动电极可以设置在流体通道的第一端和第二端。 流体通道可以包括纳米通道或微通道。 用于检测分析物的方法可以包括将分析物置于流体通道中的步骤; 沿着流体通道施加电位以在其中产生电泳力,使得分析物从流体通道的第一端转位到流体通道的第二端; 以及当分析物移动通过感测电极时,测量设置在流体通道中的一对感测电极之间的电压信号。