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1. 发明授权

US06413747B1 Enhancement of nucleic acid amplification by the addition of a polyamine 失效
标题翻译：通过添加多胺增强核酸扩增
公开(公告)号：US06413747B1
公开(公告)日：2002-07-02
申请号：US08928679
申请日：1997-09-12
申请人： Shingo Kato , Naoyuki Nishimura
发明人： Shingo Kato , Naoyuki Nishimura
IPC分类号： C12P1934
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q2527/125
摘要： If polyamine is caused to be present in the reacting system for the synthesis of nucleic acids to amplify target genes from a sample, the inhibition of the synthesis due to impurities in the sample can be reduced and the target genes can be effectively synthesized.
摘要翻译：如果在用于合成核酸的反应体系中存在多胺以从样品中扩增靶基因，则可以减少由于样品中的杂质导致的合成的抑制，并且可以有效地合成目标基因。

2. 发明授权

US5468852A Oligonucleotides for detecting bacteria 失效
标题翻译：用于检测细菌的寡核苷酸
公开(公告)号：US5468852A
公开(公告)日：1995-11-21
申请号：US932379
申请日：1992-08-19
申请人： Tetsuo Ohashi , Jun Tada , Shigeru Fukushima , Hiroko Ozaki , Naoyuki Nishimura , Yoshinari Shirasaki , Koichi Yamagata
发明人： Tetsuo Ohashi , Jun Tada , Shigeru Fukushima , Hiroko Ozaki , Naoyuki Nishimura , Yoshinari Shirasaki , Koichi Yamagata
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , Y10S435/848 , Y10S435/909
摘要： Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.
摘要翻译：可选择性地与副溶血弧菌特异性基因杂交的寡核苷酸（SEQ ID NO：1-8），与产碱性大肠杆菌线粒体的LT基因选择性杂交的寡核苷酸（SEQ ID NO：9-13），寡核苷酸（SEQ ID NO：14-21）选择性杂交与产毒性大肠杆菌的STh或STp基因，与金黄色葡萄球菌的entA，B，C或D基因选择性杂交的寡核苷酸（SEQ ID NO：22-47）或选择性与下列物质选择性杂交的寡核苷酸（SEQ ID NO：48-53）制备Staplyloccus金黄色葡萄球菌的entE基因并用作基因扩增的引物，从而仅选择性地检测引起食物中毒的相应微生物。

3. 发明授权

US07236889B2 Method of examining foreign matter derived from living body 有权
标题翻译：检查来自人体的异物的方法
公开(公告)号：US07236889B2
公开(公告)日：2007-06-26
申请号：US10370759
申请日：2003-02-24
申请人： Yoshinari Shirasaki , Naoyuki Nishimura
发明人： Yoshinari Shirasaki , Naoyuki Nishimura
IPC分类号： G06F19/00 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6827 , C12Q1/683 , C12Q1/6858
摘要： The present invention provides a method for examining a foreign matter derived from a living body in quality control for production of various products in order to rapidly identify an individual from whom a living body-derived material contaminated as a foreign matter in products or facilities involved in production of the products was derived, while securing the secret of information on nucleic acid sequences unique to individuals. The method for examining a foreign matter derived from a living body includes identifying an individual from whom a living body-derived material contaminated as a foreign matter in products or facilities involved in production of the products was derived, on the basis of information on sequences of nucleic acid contained in the living body-derived material.
摘要翻译：本发明提供了一种在生产各种产品的质量控制中检查来自生物体的异物的方法，以便快速识别在涉及的产品或设施中被污染为异物的活体衍生物质的个体产品的产生来源于确保个人独有的核酸序列信息的秘密。用于检查来自生物体的异物的方法包括根据有关序列的信息来识别从其产生的产品或设施中涉及的产品或设施中被污染为异物的生物体衍生物质的个体，包含在活体衍生物质中的核酸。

4. 发明授权

US06472187B1 Method for amplification of RNA 有权
标题翻译： RNA扩增方法
公开(公告)号：US06472187B1
公开(公告)日：2002-10-29
申请号：US09610901
申请日：2000-07-06
申请人： Hiroshi Tonoike , Naoyuki Nishimura
发明人： Hiroshi Tonoike , Naoyuki Nishimura
IPC分类号： C12P1934
CPC分类号： C12Q1/686 , C12Q2527/119 , C12Q2527/125 , C12Q2521/107
摘要： An object of the present invention is to establish a method for amplifying an RNA in a biologically-derived sample by preparing a reaction solution in which a nucleic acid synthesis is not inhibited even in the presence of various biologically-derived impurities and as well as to enable a convenient, rapid and highly sensitive analysis of the RNA in the sample. In a method of the present invention, a reaction solution having a pH higher than that of an ordinarily employed reaction solution and/or containing a polyamine and/or a sulfated polysaccharide.
摘要翻译：本发明的目的是建立一种通过制备其中核酸合成即使在各种生物衍生的杂质的存在下也不被抑制的反应溶液来扩增生物衍生的样品中的RNA的方法，以及使样品中RNA的方便，快速和高度敏感的分析。在本发明的方法中，具有pH高于通常使用的反应溶液和/或含有多胺和/或硫酸化多糖的pH的反应溶液。

5. 发明授权

US06962780B2 Method for synthesis of nucleic acids 有权
标题翻译：核酸的合成方法
公开(公告)号：US06962780B2
公开(公告)日：2005-11-08
申请号：US09843819
申请日：2001-04-30
申请人： Tomoko Nakayama , Naoyuki Nishimura
发明人： Tomoko Nakayama , Naoyuki Nishimura
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2527/125 , C12Q2527/119
摘要： The present invention is a method for synthesis of nucleic acids to amplify an intended nucleic acid in a region in which a GC content is rich, wherein a polyhydric alcohol and/or ammonium sulfate is present in an amplification reaction solution. According to the present invention, it is possible to amplify nucleic acids in a GC rich region efficiently and directly from a sample such as blood containing lots of PCR inhibitory substances without undergoing a process of isolating and purifying the nucleic acid, even though conducting PCR in the GC rich region tends to be difficult using conventional processes even if purified DNA is used.
摘要翻译：本发明是一种用于合成核酸以扩增GC含量丰富的区域中的预期核酸的方法，其中多元醇和/或硫酸铵存在于扩增反应溶液中。根据本发明，可以从含有大量PCR抑制物质的血液等有效和直接地扩增富含GC的区域中的核酸，而不进行分离纯化核酸的过程，即使进行PCR 即使使用纯化的DNA，使用常规方法的GC富集区域也难以使用。

6. 发明授权

US5525718A Oligonucleotides for detecting bacteria and detection method using same 失效
标题翻译：用于检测细菌的寡核苷酸及其检测方法
公开(公告)号：US5525718A
公开(公告)日：1996-06-11
申请号：US379296
申请日：1995-01-27
申请人： Tetsuo Ohashi , Jun Tada , Shigeru Fukushima , Hiroko Ozaki , Naoyuki Nishimura , Yoshinari Shirasaki , Koichi Yamagata
发明人： Tetsuo Ohashi , Jun Tada , Shigeru Fukushima , Hiroko Ozaki , Naoyuki Nishimura , Yoshinari Shirasaki , Koichi Yamagata
IPC分类号： C12Q1/68 , C07H21/04 , C12N1/00 , C12N15/00
CPC分类号： C12Q1/689 , Y10S435/848 , Y10S435/909
摘要： Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.
摘要翻译：可以与副溶血弧菌的特异性基因选择性杂交的寡核苷酸（SEQ ID NO：1-8），可选择性地与产生性埃希氏弧线圈的LT基因杂交的寡核苷酸（SEQ ID NO：9-13），选择性可混合的寡核苷酸（SEQ ID NO：14-21）与产毒性埃希氏胆碱圈的STh或STp基因，选择性地与金黄色葡萄球菌的entA，B，C或D基因杂交的寡核苷酸（SEQ ID NO：22-47）或选择性地与金黄色葡萄球菌选择性杂交的寡核苷酸（SEQ ID NO：48-53）制备Staplyloccus金黄色葡萄球菌的entE基因并用作基因扩增的引物，从而仅选择性地检测引起食物中毒的相应微生物。

7. 发明申请

US20100154833A1 Resist film removing apparatus, resist film removing method, organic matter removing apparatus and organic matter removing method 审中-公开
标题翻译：抗蚀剂除去装置，抗蚀剂除去方法，有机物去除装置和有机物去除方法
公开(公告)号：US20100154833A1
公开(公告)日：2010-06-24
申请号：US10510244
申请日：2003-04-15
申请人： Tamio Endo , Atsushi Sato , Yasuhiko Amano , Tetsuji Tamura , Naoyuki Nishimura , Tadahiro Ohmi , Ikunori Yokoi
发明人： Tamio Endo , Atsushi Sato , Yasuhiko Amano , Tetsuji Tamura , Naoyuki Nishimura , Tadahiro Ohmi , Ikunori Yokoi
IPC分类号： B08B3/00
CPC分类号： H01L21/6715 , B08B3/02 , B08B2203/007 , G03F7/3057 , G03F7/422 , H01L21/02052 , H01L21/31133 , H01L21/67051 , H01L21/6708
摘要： A sheetfed resist removing apparatus having a substrate (111) being a cleaning object placed therein, includes a treatment chamber (101) forming a closed space, and a spray nozzle (102) to spray a cleaning liquid in a form so-called liquid drops over a surface of the substrate (111), in which the treatment chamber (101) forms the closed space containing the substrate (111) such that the placed substrate (111) faces the spray nozzle (102). This structure allows a cleaning liquid to be in the form of liquid drops in consideration of energy reduction, and permits desirable regulation of the temperature of the liquid drops when the liquid drops contact with the resist film in spraying the cleaning liquid over the resist film on the surface of the substrate (111) to thereby remove the resist film, so that secure removal of the resist film can be accomplished.
摘要翻译：一种具有放置在其中的清洁对象的基板（111）的单张纸抗蚀剂去除装置，包括形成封闭空间的处理室（101）和喷射所谓液滴形式的清洗液的喷嘴（102）在基板（111）的表面上，处理室（101）形成包含基板（111）的封闭空间，使得放置的基板（111）面向喷嘴（102）。考虑到能量降低，这种结构允许清洗液体呈液滴形式，并且当液体在抵抗膜上喷射清洁液体时在抗蚀剂膜上喷溅时允许期望调节液滴的温度，基板（111）的表面，从而去除抗蚀剂膜，从而可以完全去除抗蚀剂膜。

8. 发明授权

US5935825A Process and reagent for amplifying nucleic acid sequences 失效
标题翻译：用于扩增核酸序列的方法和试剂
公开(公告)号：US5935825A
公开(公告)日：1999-08-10
申请号：US345393
申请日：1994-11-18
申请人： Naoyuki Nishimura , Tomoko Nakayama
发明人： Naoyuki Nishimura , Tomoko Nakayama
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , C12N1/08
CPC分类号： C12Q1/686 , Y10S435/81
摘要： This invention is directed to a novel method for PCR amplification wherein PCR is carried out at a higher pH than the pH widely used in the art. Specifically, the buffer solution is adjusted to pH 9.0 to 11.0 at 25.degree. C. Using the present invention, DNA amplification can be successfully carried out following a simple pretreatment. In the present invention whole blood is mixed with a hypotonic solution so that a selective lysis of red blood cells takes place. The residual leukocytes are then collected. The leukocytes are mixed with a polymerization agent, primers and other necessary reagents and PCR is carried out. When the PCR solution is placed at a high temperature for DNA denaturation, the leukocytes are lysed so that the leukocyte DNA is released and can access the primers and the other necessary reactions for PCR in the solution.Cell membranes and proteins are present in the PCR reaction solution due to the lack of a protein extractive step during the pretreatment. Nevertheless DNA amplification occurs under the presently claimed improved PCR method.
摘要翻译：本发明涉及用于PCR扩增的新方法，其中PCR在比本领域广泛使用的pH高的pH下进行。具体地说，将缓冲溶液在25℃下调节至pH 9.0〜11.0。使用本发明，可以在简单的预处理后成功地进行DNA扩增。在本发明中，将全血与低渗溶液混合，从而发生红细胞的选择性裂解。然后收集残留的白细胞。将白细胞与聚合剂，引物和其它必需试剂混合，进行PCR。当将PCR溶液置于高温以进行DNA变性时，将白细胞裂解，使得白细胞DNA被释放并且可以在溶液中进入引物和其它必需的PCR反应。由于在预处理期间缺乏蛋白质提取步骤，细胞膜和蛋白质存在于PCR反应溶液中。然而，DNA扩增在目前要求改进的PCR方法下进行。

9. 发明申请

US20090269745A1 RNA EXTRACTION METHOD AND RNA DETECTION METHOD 审中-公开
标题翻译： RNA提取方法和RNA检测方法
公开(公告)号：US20090269745A1
公开(公告)日：2009-10-29
申请号：US12092067
申请日：2006-11-02
申请人： Hiroshi Tonoike , Yoshinari Shirasaki , Naoyuki Nishimura , Shigeru Tamatsukuri , Kuhomi Watanabe , Yasuhiko Sakakura , Hiroyuki Nakayama
发明人： Hiroshi Tonoike , Yoshinari Shirasaki , Naoyuki Nishimura , Shigeru Tamatsukuri , Kuhomi Watanabe , Yasuhiko Sakakura , Hiroyuki Nakayama
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101
摘要： The present invention provides a method for inactivating RNase which generally presents in a sample such as biological sample (especially an excrement sample), or in a sample such as a living body-derived sample (especially an excrement-derived sample) obtained by separation of an RNA-including body therefrom or the like; a method for extracting and detecting RNA from the sample. An RNA extraction method, comprising the steps of: obtaining a mixture under a heating condition, said mixture comprising: a sample comprising an RNA-including body and RNase, and an alkaline treating reagent comprising at least a reducing agent, and having pH of 8.1 or higher, and conducting inactivation of the RNase and extraction of RNA from the RNA-including body by keeping the mixture under the heating condition. An RNA detection method, comprising conducting RNA amplification reaction by mixing a treated sample liquid comprising RNA extracted by the extraction method and an amplification reaction solution.
摘要翻译：本发明提供了通常存在于样品例如生物样品（特别是排泄物样品）中的RNase的灭活方法，或者通过分离获得的样品例如来自活体的样品（特别是排泄物衍生的样品）来自其的含RNA的体; 从样品中提取和检测RNA的方法。一种RNA提取方法，包括以下步骤：在加热条件下获得混合物，所述混合物包含：包含含RNA的体和RNase的样品和至少包含还原剂的碱处理试剂，pH为8.1 或更高，并且通过将混合物保持在加热条件下，使RNase失活并从含RNA的体内提取RNA。一种RNA检测方法，包括通过混合包含通过提取方法提取的RNA的经处理的样品液体和扩增反应溶液进行RNA扩增反应。

10. 发明授权

US5912146A Method for synthesis of nucleic acids 失效
标题翻译：核酸的合成方法
公开(公告)号：US5912146A
公开(公告)日：1999-06-15
申请号：US911735
申请日：1997-08-15
申请人： Naoyuki Nishimura , Reiko Yoshida
发明人： Naoyuki Nishimura , Reiko Yoshida
IPC分类号： G01N33/50 , C07H21/04 , C12N15/09 , C12Q1/68 , C12P19/34 , C12N9/28
CPC分类号： C12Q1/6806
摘要： The present invention provides a method of nucleic acid synthesis capable of directly amplifying a gene of interest in a salivary sample without purifying DNAs from the sample.According to the present invention, in a method of nucleic acid synthesis by mixing a salivary sample itself and a gene amplification reaction solution, and then subjecting them to an amplification reaction, the salivary sample is heat-treated or treated with a saccharide-degrading enzyme before the reaction. For example, a salivary sample was heat-treated at 0-40.degree. C. for 1 hour, subsequently added directly into a PCR reaction solution, and then subjected to a PCR. The result demonstrates that, as shown in FIG. 3, the amount of the PCR product increased according as the heat-treatment temperature was elevated, and as a consequence, the PCR product sufficient to be detected in the electrophoresis was produced in each of all cases using different amounts of saliva, by heat-treating the sample at 30.degree. C. or above.
摘要翻译：本发明提供了能够直接扩增唾液样品中目标基因而不从样品中纯化DNA的核酸合成方法。根据本发明，在通过将唾液样品本身与基因扩增反应溶液混合，然后对其进行扩增反应的核酸合成方法中，对唾液样品进行热处理或用糖降解酶处理在反应之前。例如，将唾液样品在0-40℃下热处理1小时，随后直接加入PCR反应溶液中，然后进行PCR。结果表明，如图1所示。 3，随着热处理温度的升高，PCR产物的量增加，结果，在使用不同量的唾液的所有情况下，通过热处理产生足以在电泳中检测的PCR产物，在30℃或更高温度下处理样品。

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