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1. 发明授权

US10745728B1 Applications for biversal nucleoside analogs 有权
公开(公告)号：US10745728B1
公开(公告)日：2020-08-18
申请号：US16113766
申请日：2018-08-27
申请人： Steven A Benner , Hyo-Joong Kim , Zunyi Yang
发明人： Steven A Benner , Hyo-Joong Kim , Zunyi Yang
IPC分类号： C07H19/00 , C07H21/00 , C12P19/34 , C12Q1/686 , C07H19/04 , C07H21/02 , C07H21/04
摘要： This invention relates to the use of analogs of purines that have two tautomeric forms, one formally complementary to thymidine, the other formally complementary to cytidine, and analogs of pyrimidines that have two tautomeric forms, one formally complementary to guanosine, the other formally complementary to adenosine. These uses include the polymerase chain reaction amplification of targets having polymorphisms in primer binding sites and ligation.

2. 发明申请

US20150050695A1 Helicase Dependent Amplification of DNA Molecules Using Nucleotide Analogs 审中-公开
标题翻译：使用核苷酸类似物的DNA分子的Helicase依赖性扩增
公开(公告)号：US20150050695A1
公开(公告)日：2015-02-19
申请号：US13970111
申请日：2013-08-19
申请人： Steven A. Benner , Zunyi Yang
发明人： Steven A. Benner , Zunyi Yang
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2521/513 , C12Q2525/101 , C12Q2525/117 , C12Q2521/101
摘要： This invention covers processes for the isothermal amplification of DNA molecules having a preselected sequence. It is based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine by inosine, thymine by 2-thiothymine, and cytosine by N4-ethylcytosine (“SAMRS nucleotides”) were accepted by enzymes used in the standard helicase-dependent amplification (HDA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an HDA-like process (hereinafter abbreviated as simply HDA) using substituted primers. Also discovered was the diminution of spurious products through the use of SAMRS-substituted primers.
摘要翻译：本发明涉及具有预选序列的DNA分子的等温扩增方法。基于意想不到的发现，在某些位置，由2-氨基嘌呤或二氨基嘌呤取代的腺嘌呤，由肌苷引起的鸟嘌呤，2-硫代胸腺嘧啶的胸腺嘧啶和N4-乙基胞嘧啶的胞嘧啶（“SAMRS核苷酸”）的引物被酶用于标准解旋酶依赖性扩增（HDA）。进一步意外的是，使用取代的引物，以HDA样方法（以下简称为HDA）有效扩增靶核苷酸。还发现通过使用SAMRS取代的引物减少杂质产物。

3. 发明授权

US10415088B1 In vivo conversion of nucleosides in plasmid DNA 有权
公开(公告)号：US10415088B1
公开(公告)日：2019-09-17
申请号：US15997325
申请日：2018-06-04
申请人： Steven A Benner , Zunyi Yang
发明人： Steven A Benner , Zunyi Yang
IPC分类号： C12Q1/68 , C12Q1/6869 , C12P19/34 , C12Q1/6844 , C07H21/04
摘要： Disclosed are processes that use DNA polymerases to extend primers annealed to templates, wherein a standard nucleotide in the template guiding the extension directs the incorporation of a nonstandard nucleotide analog into the product duplex opposite that standard template nucleotide, and processes wherein a nonstandard nucleotide in the template guiding the extension directs the incorporation of a standard nucleotide analog opposite the nonstandard template nucleotide. Conditions, including the pH of the mixture where the primer extension is done and the composition of triphosphate mixtures where the primary extensions done, are disclosed.

4. 发明申请

US20220162600A1 Compositions for the Multiplexed Detection of Viruses 有权
公开(公告)号：US20220162600A1
公开(公告)日：2022-05-26
申请号：US17101467
申请日：2020-11-23
申请人： Zunyi Yang , Steven A Benner
发明人： Zunyi Yang , Steven A Benner
IPC分类号： C12N15/113 , C12N9/12 , C12Q1/686 , C12Q1/6853
摘要： This specification discloses compositions of matter and processes that allow the detection of RNA from coronaviruses and other RNA viruses, in particular, compositions and processes that have the capacity to detect in multiplexed form many RNA targets within individual viruses, targets from multiple viruses, and other RNA molecules that can be used as positive controls.

5. 发明授权

US09725713B1 In vitro selection with expanded genetic alphabets 有权
公开(公告)号：US09725713B1
公开(公告)日：2017-08-08
申请号：US14082800
申请日：2013-11-18
申请人： Steven A Benner , Zunyi Yang
发明人： Steven A Benner , Zunyi Yang
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1048 , C12Q1/6811 , C12Q2521/345 , C12Q2525/117 , C12Q2525/205
摘要： This invention provides for products and processes for binding to a preselected target, where the process involves contacting this target to an oligonucleotide molecule that contains one or more “non-standard” nucleotides, which are nucleotide analogs that, when incorporated into oligonucleotides (DNA or RNA, collectively xNA), present to a complementary strand in a Watson-Crick pairing geometry a pattern of hydrogen bonds that is different from the pattern presented by adenine, guanine, cytosine, and uracil. This disclosure provides an example where such an oligonucleotide molecule contains a single 2-amino-8-(1′-β-D-2′-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)one and a single 6-amino-5-nitro-3-(1′-β-D-2′-deoxyribofuranosyl)-2(1H)-pyridone, and where the target is a cell, and is obtained by a process of in vitro selection.

6. 发明授权

US09315842B2 Helicase dependent amplification of DNA molecules using nucleotide analogs 有权
标题翻译：使用核苷酸类似物的DNA分子的螺旋酶依赖性扩增
公开(公告)号：US09315842B2
公开(公告)日：2016-04-19
申请号：US13970111
申请日：2013-08-19
申请人： Steven A Benner , Zunyi Yang
发明人： Steven A Benner , Zunyi Yang
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2521/513 , C12Q2525/101 , C12Q2525/117 , C12Q2521/101
摘要： This invention covers processes for the isothermal amplification of DNA molecules having a preselected sequence. It is based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine by inosine, thymine by 2-thiothymine, and cytosine by N4-ethylcytosine (“SAMRS nucleotides”) were accepted by enzymes used in the standard helicase-dependent amplification (HDA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an HDA-like process (hereinafter abbreviated as simply HDA) using substituted primers. Also discovered was the diminution of spurious products through the use of SAMRS-substituted primers.
摘要翻译：本发明涉及具有预选序列的DNA分子的等温扩增方法。基于意想不到的发现，在某些位置，由2-氨基嘌呤或二氨基嘌呤取代的腺嘌呤，由肌苷引起的鸟嘌呤，2-硫代胸腺嘧啶的胸腺嘧啶和N4-乙基胞嘧啶的胞嘧啶（“SAMRS核苷酸”）的引物被酶用于标准解旋酶依赖性扩增（HDA）。进一步意外的是，使用取代的引物，以HDA样方法（以下简称为HDA）有效扩增靶核苷酸。还发现通过使用SAMRS取代的引物减少杂质产物。

7. 发明授权

US10829812B1 Amplification of oligonucleotides containing non-standard nucleotides 有权
公开(公告)号：US10829812B1
公开(公告)日：2020-11-10
申请号：US16216266
申请日：2018-12-11
申请人： Steven A Benner , Roberto Laos , Nicole A Leal , Zunyi Yang , Myong Jung Kim
发明人： Steven A Benner , Roberto Laos , Nicole A Leal , Zunyi Yang , Myong Jung Kim
IPC分类号： C12Q1/686 , C12Q1/6853 , C12Q1/6806
摘要： This invention relates to processes that amplify oligonucleotide analogs that incorporate non-standard nucleobase analogs from an artificially expanded genetic information system. These pair in DNA duplexes via patterns of hydrogen bonds that differ from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.

8. 发明授权

US09637783B1 Amplification of oligonucleotides containing non-standard nucleotides by polymerase chain reactions 有权
公开(公告)号：US09637783B1
公开(公告)日：2017-05-02
申请号：US14746243
申请日：2015-06-22
申请人： Steven Benner , Roberto Laos , Nicole Leal , Nilesh Karalkar , Zunyi Yang
发明人： Steven Benner , Roberto Laos , Nicole Leal , Nilesh Karalkar , Zunyi Yang
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/12
CPC分类号： C12Q1/6869 , C12N9/1252 , C12P19/34 , C12Q1/6844 , C12Q2525/117 , C12Y207/07007 , C12Q2525/101
摘要： This invention relates to processes that amplify, in a polymerase chain reaction architecture, oligonucleotide analogs that incorporate non-standard nucleobase analogs from an artificially expanded genetic information system. These pair in DNA duplexes via patterns of hydrogen bonds that are different from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.

9. 发明申请

US20110124053A1 POLYMERASE INCORPORATION OF NON-STANDARD NUCLEOTIDES 有权
标题翻译：聚合物纳入非标准核素
公开(公告)号：US20110124053A1
公开(公告)日：2011-05-26
申请号：US12999138
申请日：2009-06-16
申请人： Steven Albert Benner , Fei Chen , Zunyi Yang
发明人： Steven Albert Benner , Fei Chen , Zunyi Yang
IPC分类号： C12P19/34
CPC分类号： C12Q1/6853 , C12P19/34 , C12Q1/686 , C12Q2521/101 , C12Q2525/117 , C12Q2525/191
摘要： The disclosed invention teaches processes to amplify oligonucleotides by contacting templates and primers with DNA polymerases and triphosphates of non-standard nucleotides, which form nucleobase pairs fitting the standard Watson-Crick geometry, but joined by hydrogen bonding patterns different from those that join standard A:T and G:C pairs. Thus, this invention relates to nucleotide analogs and their derivatives that, when incorporated into DNA and RNA, expand the number of replicatable nucleotides beyond the four found in standard DNA and RNA. The invention further relates to polymerases that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives, and more specifically, polymerases and non-standard nucleoside triphosphates that support the polymerase chain reaction (PCR), including PCR where the products contain more than one non-standard nucleotide unit. Examples are provides that show this process using 6-amino-5-nitro-3-(1′-beta-D-2′-deoxyribofuranosyl)-2(1H)-pyridone to implement the non-standard “small” donor-donor-acceptor (pyDDA) hydrogen bonding pattern, and 2-amino-8-(r-beta-D-2′-deoxyribofuranosyl)-imidazo[1,2-α]-1,3,5-triazin-4(8H)-one to implement the “large” acceptor-acceptor-donor (puADD) pattern.
摘要翻译：所公开的发明教导了通过使模板和引物与DNA聚合酶和非标准核苷酸的三磷酸接触来扩增寡核苷酸的过程，其形成适合标准Watson-Crick几何形状的核碱基对，但是通过与加入标准A： T和G：C对。因此，本发明涉及核苷酸类似物及其衍生物，其在掺入DNA和RNA时扩大超出标准DNA和RNA中发现的四种可重复核苷酸的数量。本发明还涉及使用相应的三磷酸衍生物，更具体地，支持聚合酶链式反应（PCR）的聚合酶和非标准核苷三磷酸（包括产物含有的PCR）的寡核苷酸产物，将这些非标准核苷酸类似物结合到聚合酶中的聚合酶多个非标准核苷酸单位。提供了使用6-氨基-5-硝基-3-（1'-β-D-2'-脱氧三呋喃糖基）-2（1H） - 吡啶酮来实施非标准“小”供体 - 供体 - 受体（pyDDA）氢键图案和2-氨基-8-（r-β-D-2'-脱氧三呋喃糖基） - 咪唑并[1,2-α] -1,3,5-三嗪-4（8H） - 实施“大”受体 - 受体 - 供体（puADD）模式。

10. 发明申请

US20170158275A1 ASSEMBLED DUAL-USE BALANCE CAR 审中-公开
公开(公告)号：US20170158275A1
公开(公告)日：2017-06-08
申请号：US15149184
申请日：2016-05-09
申请人： Zunyi Yang
发明人： Zunyi Yang
IPC分类号： B62K3/00 , B62K21/16
CPC分类号： B62K21/16 , B62K11/007 , B62K21/12
摘要： An assembled dual-use balance car, comprises balance car body comprising a first foot board, a second foot board, a first and a second wheel, a first and a second motor respectively, and a balance control system, the balance car body being moved forward, draw back, and turns by tilting the first and second foot board forward and backward, and rotating around each other; a steering assembly comprising a locking part configured to prevent the first and second foot board from being rotating around each other, and a sensor assembly disposed at a bottom end of the steering rod and configured as a steering control of the balance car body, the sensor assembly being electrically connected with the balance control system; the steering assembly can be assembled and disassembled flexibly in the assembled dual-use balance car based on actual using situation.

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