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    • 4. 发明申请
    • GENE EXPRESSION INHIBITOR SELECTIVE FOR MATRIX METALLOPROTEINASE-9 GENE
    • 基因表达抑制剂选择性基因金属蛋白酶-9基因
    • US20110046070A1
    • 2011-02-24
    • US12988163
    • 2009-04-17
    • Hiroki NagaseXiaofei Wang
    • Hiroki NagaseXiaofei Wang
    • A61K38/10C07K7/08A61P35/00C12N5/00
    • C07D403/14A61K31/787
    • Disclosed are: an inhibitor of the expression of matrix metalloproteinase-9 gene; a therapeutic agent for a disease associated with matrix metalloproteinase-9; and a carcinostatic agent. Each of the agents comprises a pyrrole-imidazole polyamide having an N-methylpyrrole unit, an N-methylimidazole unit and a γ-aminobutyric acid unit. The pyrrole-imidazole polyamide can be folded at the site of the γ-aminobutyric acid unit to form a U-shaped conformation in a minor groove of a double-stranded domain comprising a part or the whole of a specific nucleotide sequence (SEQ ID NO:2, SEQ ID NO:4) included in a human matrix metalloproteinase-9 gene promoter and a strand complementary to the part or the whole of the specific nucleotide sequence. In the U-shaped conformation, a Py-Im pair, an Im/Py pair and a Py/Py pair in the pyrrole-imidazole polyamide target a C-G base pair, a G-C base pair, and both of an A-T base pair and a T-A base pair in the minor groove, respectively.
    • 公开了:基质金属蛋白酶-9基因表达的抑制剂; 用于与基质金属蛋白酶-9相关的疾病的治疗剂; 和制癌剂。 每种试剂包括具有N-甲基吡咯单元,N-甲基咪唑单元和γ-氨基丁酸单元的吡咯 - 咪唑聚酰胺。 吡咯 - 咪唑聚酰胺可以在γ-氨基丁酸单元的位置处折叠以在双链结构域的小沟中形成U形构象,其包含部分或全部特定核苷酸序列(SEQ ID NO :2,SEQ ID NO:4),以及与该特定核苷酸序列的一部分或全部互补的链。 在U形构象中,吡咯 - 咪唑聚酰胺靶中的Py-Im对,Im / Py对和Py / Py对为CG碱基对,GC碱基对,AT碱基对和 分别在小槽中的TA碱基对。