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2. 发明授权

US07393633B1 Genotyping kit for diagnosis of human papillomavirus infection 失效
标题翻译：用于诊断人乳头瘤病毒感染的基因分型试剂盒
公开(公告)号：US07393633B1
公开(公告)日：2008-07-01
申请号：US09807234
申请日：2000-10-26
申请人： Tae-Shin Park , Mi-Sun Park , Jeongmi Kim
发明人： Tae-Shin Park , Mi-Sun Park , Jeongmi Kim
IPC分类号： C12Q1/68 , C12P19/34 , G01N33/566 , G01N30/96
CPC分类号： C12Q1/708 , C12Q1/6886
摘要： The present invention relates to a genotyping kit for diagnosing patients infected with human papillomavirus (HPV), and a method for diagnosis of HPV infection by genotyping specimen DNA isolated from the patients using the said kit. The genotyping kit of the invention comprises a DNA chip with probes that have nucleotide sequences complementary to DNA of HPV, primers for amplifying the DNA of a sample by PCR, and means for labeling hybridized sample DNA with the DNA chip. The method for diagnosis of HPV infection comprises the steps of amplifying DNA obtained from a sample using the primers of the kit, applying the amplified DNA to the DNA chip and hybridizing the amplified DNA and the probes of the DNA chip, and detecting DNA bound on the surface of the DNA chip by labeling hybridized DNA. In accordance with the invention, the genotyping kit may be practically applied to the early diagnosis, prevention and treatment of cervical cancer, since the kit can easily diagnose HPV infection, and can exactly determine the genotype of the HPV.
摘要翻译：本发明涉及用于诊断感染人乳头状瘤病毒（HPV）的患者的基因分型试剂盒，以及使用所述试剂盒从患者分离的基因分型标本DNA来诊断HPV感染的方法。本发明的基因分型试剂盒包含具有与HPV的DNA互补的核苷酸序列的探针的DNA芯片，通过PCR扩增样品的DNA的引物，以及用DNA芯片标记杂交样品DNA的方法。用于诊断HPV感染的方法包括以下步骤：使用试剂盒的引物扩增从样品获得的DNA，将扩增的DNA应用于DNA芯片，并将扩增的DNA与DNA芯片的探针杂交，并检测DNA结合的DNA 通过标记杂交DNA的DNA芯片的表面。根据本发明，基因分型试剂盒可以实际应用于子宫颈癌的早期诊断，预防和治疗，因为试剂盒可以容易地诊断HPV感染，并且可以准确地确定HPV的基因型。

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