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1. 发明授权

US09738908B2 CRISPR/Cas systems for genomic modification and gene modulation 无效
公开(公告)号：US09738908B2
公开(公告)日：2017-08-22
申请号：US14216655
申请日：2014-03-17
申请人： System Biosciences, LLC
发明人： Fangting Wu
IPC分类号： C12N9/22 , C12N9/14 , C12N15/90 , C12N15/85 , C12N15/10 , C12N15/11
CPC分类号： C12N15/907 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/85 , C12N2800/22
摘要： The invention relates to engineered CRISPR/Cas9 systems for genomic modification and regulation of gene expression in mammalian cells. The specification describes the design and validation of polynucleotides encoding the Streptococcus pyogenes (S. pyogenes) Cas9 gene and protein and variants of that protein, where the nucleotide sequence has been optimized for expression in mammalian cells, and also modified by fused sequences that enhance various aspects of the CRISPR/Cas system. The specification also describes systems for RNA-guided genome engineering and gene regulation in mammalian cells, including human cells.

2. 发明授权

US10202619B2 Compositions and methods directed to CRISPR/Cas genomic engineering systems 有权
公开(公告)号：US10202619B2
公开(公告)日：2019-02-12
申请号：US14875282
申请日：2015-10-05
申请人： System Biosciences, LLC
发明人： Fangting Wu
IPC分类号： C12N9/22 , C12N15/10 , C12N15/11 , C12N15/85 , C12N15/90
摘要： The invention relates to engineered CRISPR/Cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the Streptococcus pyogenes (S. pyogenes) Cas9 protein, where the nucleotide sequence has been optimized for expression in mammalian cells. The specification also describes all-in-one systems for RNA-guided genome engineering in mammalian cells, including human cells.

3. 发明授权

US09234213B2 Compositions and methods directed to CRISPR/Cas genomic engineering systems 有权
标题翻译：针对CRISPR / Cas基因组工程系统的成分和方法
公开(公告)号：US09234213B2
公开(公告)日：2016-01-12
申请号：US14211858
申请日：2014-03-14
申请人： System Biosciences, LLC
发明人： Fangting Wu
IPC分类号： C12N15/90 , C12N9/22 , C12N15/85 , C12N15/10
CPC分类号： C12N15/907 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/85 , C12N2800/22
摘要： The invention relates to engineered CRISPR/Cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the Streptococcus pyogenes (S. pyogenes) Cas9 protein, where the nucleotide sequence has been optimized for expression in mammalian cells. The specification also describes all-in-one systems for RNA-guided genome engineering in mammalian cells, including human cells.
摘要翻译：本发明涉及用于哺乳动物细胞中基因组修饰的工程化CRISPR / Cas9系统。本说明书描述了编码化脓性链球菌（S. pyogenes）Cas9蛋白的多核苷酸的设计和测试，其中核苷酸序列已被优化用于在哺乳动物细胞中表达。本说明书还描述了哺乳动物细胞（包括人类细胞）中RNA引导基因组工程的一体化系统。

4. 发明授权

US09005888B2 Methods for microvesicle isolation and selective removal 有权
标题翻译：微泡分离和选择性去除方法
公开(公告)号：US09005888B2
公开(公告)日：2015-04-14
申请号：US13918260
申请日：2013-06-14
申请人： System Biosciences, LLC
发明人： Travis John Antes , Kevin Kwei , Fangting Wu
IPC分类号： C12Q1/00 , C12Q1/02 , G01N1/34 , C12Q1/68 , G01N33/50 , G01N33/52 , C12M1/00
CPC分类号： G01N1/34 , C12M29/26 , C12Q1/6895 , C12Q2600/158 , C12Q2600/178 , G01N33/5002 , G01N33/52 , C12Q1/6806 , C12Q2523/32 , C12Q2563/161
摘要： The invention relates to compositions and methods for isolation of microvesicles produced by mammalian cells. These microvesicles, known as extracellular microvesicles or circulating microvesicles, are isolated from sample materials such as body fluids, or from cell culture media that has been used to culture and maintain mammalian cells in vitro. The isolation of microvesicles as described herein results in purification and concentration of the microvesicles.The invention also provides related methods for producing blood serum and/or blood plasma that is free of detectable microvesicles, largely depleted of microvesicles, or has reduced concentration of microvesicles compared to the blood serum or blood plasma starting material (collectively termed “microvesicle-depleted”). The generation of microvesicle-depleted blood serum or plasma is critical for use in experimental systems where it is desirable to use a cell culture media that does not contain endogenous microvesicles, or has been depleted of endogenous microvesicles, from the source material.
摘要翻译：本发明涉及用于分离由哺乳动物细胞产生的微泡的组合物和方法。称为细胞外微泡或循环微泡的这些微泡从样品材料如体液中分离出来，或从用于在体外培养和维持哺乳动物细胞的细胞培养基中分离。如本文所述分离微泡导致微泡的纯化和浓缩。本发明还提供了相对于血清和/或血浆的相关方法，其不含可检测的微泡，大部分耗尽微泡，或者与血清或血浆起始物质（统称为“微泡消耗的 “）。对于在实验系统中使用的微泡结蛋白血清或血浆的产生是至关重要的，其中期望使用不含有内源性微泡或已经从源材料中消耗内源性微泡的细胞培养基。

5. 发明申请

US20140273226A1 CRISPR/CAS SYSTEMS FOR GENOMIC MODIFICATION AND GENE MODULATION 无效
标题翻译：用于基因修饰和基因调控的CRISPR / CAS系统
公开(公告)号：US20140273226A1
公开(公告)日：2014-09-18
申请号：US14216655
申请日：2014-03-17
申请人： System Biosciences, LLC
发明人： Fangting Wu
IPC分类号： C12N15/85
CPC分类号： C12N15/907 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/85 , C12N2800/22
摘要： The invention relates to engineered CRISPR/Cas9 systems for genomic modification and regulation of gene expression in mammalian cells. The specification describes the design and validation of polynucleotides encoding the Streptococcus pyogenes (S. pyogenes) Cas9 gene and protein and variants of that protein, where the nucleotide sequence has been optimized for expression in mammalian cells, and also modified by fused sequences that enhance various aspects of the CRISPR/Cas system. The specification also describes systems for RNA-guided genome engineering and gene regulation in mammalian cells, including human cells.
摘要翻译：本发明涉及用于基因组修饰和哺乳动物细胞中基因表达调节的工程化CRISPR / Cas9系统。本说明书描述了编码化脓性链球菌（S. pyogenes）Cas9基因的多核苷酸和该蛋白质的变体的设计和验证，其中核苷酸序列已经优化用于在哺乳动物细胞中表达，并且还通过增强各种功能的融合序列修饰 CRISPR / Cas系统的各个方面。该说明书还描述了用于RNA引导的基因组工程和哺乳动物细胞（包括人细胞）中基因调控的系统。

6. 发明申请

US20140273037A1 COMPOSITIONS AND METHODS DIRECTED TO CRISPR/CAS GENOMIC ENGINEERING SYSTEMS 有权
标题翻译：组合物和方法指导CRISPR / CAS基因工程系统
公开(公告)号：US20140273037A1
公开(公告)日：2014-09-18
申请号：US14211858
申请日：2014-03-14
申请人： System Biosciences, LLC
发明人： Fangting Wu
IPC分类号： C12N15/90 , C12N9/22
CPC分类号： C12N15/907 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/85 , C12N2800/22
摘要： The invention relates to engineered CRISPR/Cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the Streptococcus pyogenes (S. pyogenes) Cas9 protein, where the nucleotide sequence has been optimized for expression in mammalian cells. The specification also describes all-in-one systems for RNA-guided genome engineering in mammalian cells, including human cells.
摘要翻译：本发明涉及用于哺乳动物细胞中基因组修饰的工程化CRISPR / Cas9系统。本说明书描述了编码化脓性链球菌（S. pyogenes）Cas9蛋白的多核苷酸的设计和测试，其中核苷酸序列已被优化用于在哺乳动物细胞中表达。本说明书还描述了哺乳动物细胞（包括人类细胞）中RNA引导基因组工程的一体化系统。

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