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    • 8. 发明授权
    • Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants
    • 核酸分子SEQ ID NO: 68811和与植物相关的其他分子
    • US07560542B2
    • 2009-07-14
    • US11353150
    • 2006-02-14
    • Scott E. AndersenTimothy W. ConnerRaghunath V. Lalgudi
    • Scott E. AndersenTimothy W. ConnerRaghunath V. Lalgudi
    • A01H5/00C07H21/04C12N15/05C12N15/82
    • C07K14/415C12N15/8242C12N15/8261Y02A40/146
    • Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.
    • 公开了从玉米分离的表达序列标签(ESTs)。 EST为靶基因和分离新基因提供了独特的分子工具,用于植物保护和改良。 所公开的EST可用于开发用于了解关键植物发育和代谢途径的新策略。 所公开的EST在分离基因和启动子方面具有特别的用途,鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 使用本发明提供的EST的序列同源性分析将导致对所需农学性状的更有效的基因筛选。 这些植物基因组学难题的这些精选片段的不断扩展的数据库将快速扩展后续功能验证所必需的知识,这是当前植物生物技术努力的关键限制。
    • 10. 发明授权
    • Strawberry promoters and genes
    • 草莓启动子和基因
    • US6080914A
    • 2000-06-27
    • US8979
    • 1998-01-20
    • Timothy W. Conner
    • Timothy W. Conner
    • C07K14/415C12N9/04C12N9/42C12N15/29C12N15/82A01H5/00C07H21/04C12N5/14
    • C12N9/2437C07K14/415C12N15/8222C12N15/8235C12N15/8249C12N15/8279C12N9/0006C12Y302/01004
    • Promoters and genes isolated from genomic DNA of strawberry plants are disclosed. Both the promoters and genes are capable of tissue-specific expression in transgenic plants. A plant promoter that is a nucleic acid region located upstream of the 5' end of a plant DNA structural coding sequence that is transcribed at high levels in ripening fruit tissue. This promoter region is capable of conferring high levels of transcription in ripening fruit tissue and in developing seed tissues when used as a promoter for a heterologous coding sequence in a chimeric gene. The promoter and any chimeric gene in which it may be used can be used to obtain transformed plants or plant cells. A DNA coding sequence that codes for a gene that is highly transcribed in ripening fruit tissue of Fragaria X ananassa. This coding sequence can be used to obtain a cDNA probe useful in obtaining analogous promoters from a homologous coding sequence in other plant species. Chimeric genes including the isolated promoter region, transformed plants containing the isolated promoter region, transformed plant cells and seeds are also disclosed.
    • 公开了从草莓植物的基因组DNA分离的启动子和基因。 启动子和基因都能够在转基因植物中进行组织特异性表达。 植物启动子,其是位于成熟果实组织中高水平转录的植物DNA结构编码序列的5'末端上游的核酸区域。 当作为嵌合基因中的异源编码序列的启动子时,该启动子区域能够在成熟果实组织和发育中的种子组织中赋予高水平的转录。 可以使用其可以使用的启动子和任何嵌合基因来获得转化的植物或植物细胞。 编码在Fragaria X ananassa的成熟果实组织中高度转录的基因的DNA编码序列。 该编码序列可用于获得可用于从其他植物物种中的同源编码序列获得类似启动子的cDNA探针。 还公开了包括分离的启动子区域的嵌合基因,含有分离的启动子区域的转化植物,转化的植物细胞和种子。