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1. 发明授权

US08372628B2 Sulfur-oxidizing bacteria and their use in bioleaching processes for sulfured copper minerals 有权
标题翻译：硫氧化细菌及其在硫化铜矿物质生物浸出过程中的应用
公开(公告)号：US08372628B2
公开(公告)日：2013-02-12
申请号：US12693004
申请日：2010-01-25
申请人： Atsuko Ohata , Manabu Manabe , Pilar A. Parada Valdecantos
发明人： Atsuko Ohata , Manabu Manabe , Pilar A. Parada Valdecantos
IPC分类号： C10G32/00
CPC分类号： C22B3/18 , C22B15/0063 , C22B15/0071 , Y02P10/234 , Y02P10/236
摘要： The present invention is related to an isolated chemolithotrophic bacterium belonging to species Acidithiobacillus thiooxidans named Licanantay, deposited in Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH-DSMZ with number DSM 17318, and its use in pure form or in mixtures that contains it for bioleaching processes of minerals or sulfured metallic species concentrates. This Licanantay strain DSM 17318 has sulfur-oxidizing activity in both primary and secondary sulfured minerals, especially in the case of chalcopyrite, covellite, bornite, chalcocite, enargite and tennantite.
摘要翻译：本发明涉及一种名为Licanantay的嗜酸硫杆菌硫代氧化物的物种的分离的化学嗜热细菌，其存放在具有数字DSM17318的德国三明治米诺克氏菌素和Zellkulturen GmbH-DSMZ中，其用于纯形式或含有它的混合物，用于生物浸出过程矿物质或硫化金属物质浓缩物。这种Licanantay菌株DSM 17318在初级和次级硫化矿物中都具有硫氧化活性，特别是在黄铜矿，碳黑，斑铜矿，辉铜矿，榴辉岩和铁锰矿的情况下。

2. 发明申请

US20120208257A1 ADDITIVE FOR BIOLEACHING THAT IS SUBSTANTIALLY MADE UP OF THE LICANANTASE LIPOPROTEIN, AND BIOLEACHING PROCESS TO WHICH THIS ADDITIVE IS ADDED TO INCREASE THE RECOVERY OF COPPER 有权
标题翻译：添加生物利用物质的添加剂，以及添加剂添加剂以增加铜的回收率的生物工艺
公开(公告)号：US20120208257A1
公开(公告)日：2012-08-16
申请号：US13390488
申请日：2010-08-13
申请人： Roberto Bobadilla Fazzini , Pilar Angélica Parada Valdecantos , Ricardo Badilla Ohlbaum
发明人： Roberto Bobadilla Fazzini , Pilar Angélica Parada Valdecantos , Ricardo Badilla Ohlbaum
IPC分类号： C12N9/00 , C12S99/00
CPC分类号： C21B15/00 , C07K14/195 , C22B3/18 , Y02P10/234
摘要： The invention discloses an additive for bioleaching that makes it possible to increase the recovery of copper from sulfide ores. In which this additive is substantially made up of the Licanantase lipoprotein and a solution of sulfuric acid with a pH of 0.8 to 3. The Licanantase lipoprotein that has an amino acid sequence with at least 50% homology regarding the sequence defined in SEQ ID No. 1 or is the product of translation of a nucleotide sequence with at least 50% homology regarding the sequence defined in SEQ ID No. 2. It also protects the improved bioleaching process that includes adding the additive during the ore bioleaching process as defined in the present invention; and continuing with the habitual process, obtaining copper recoveries increased 5 to 20%.
摘要翻译：本发明公开了一种用于生物浸出的添加剂，使得可以增加硫化物矿石中铜的回收率。其中该添加剂基本上由Licanantase脂蛋白和pH为0.8至3的硫酸溶液组成。具有与SEQ ID No.中定义的序列具有至少50％同源性的氨基酸序列的Licanantase脂蛋白 1或者是与SEQ ID No.2定义的序列具有至少50％同源性的核苷酸序列的翻译产物。它还保护改进的生物浸出过程，其包括在本发明定义的矿石生物浸出过程中添加添加剂发明; 并继续采用惯例，铜回收率提高5〜20％。

3. 发明申请

US20070059743A1 Method for the design of oligonucleotides for molecular biology techniques 有权
标题翻译：分子生物学技术寡核苷酸设计方法
公开(公告)号：US20070059743A1
公开(公告)日：2007-03-15
申请号：US11506089
申请日：2006-08-17
申请人： Alejandro Maass Sepulveda , Andres Aravena Duarte , Mauricio Gonzalez Canales , Servet Martinez Aguilera , Pilar Parada Valdecantos , Katia Ehrenfeld Stolzenbach
发明人： Alejandro Maass Sepulveda , Andres Aravena Duarte , Mauricio Gonzalez Canales , Servet Martinez Aguilera , Pilar Parada Valdecantos , Katia Ehrenfeld Stolzenbach
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： G06F19/20
摘要： The present invention discloses a method that can be used to identify one DNA sequence or one specific group of DNA sequences from a complex biological sample. Diverse molecular biology methods require the use of short DNA sequences, called oligonucleotides, that are artificially synthesized from a description of their composing bases. The disclosed method allows the design of oligonucleotides useful for said molecular biology procedures, like probe design procedures, and is characterized by the construction of a database of reference sequences, the selection of a subset of sequences belonging to target organisms, the selection of candidate oligonucleotides from such sequences, the depuration of these candidate oligonucleotides according to hybridization specificity and thermodynamic stability criteria, and the sorting of such oligonucleotides according to their taxonomic specificity. In a second aspect, a method is disclosed to design oligonucleotides pairs or primers, which are required in certain molecular biology techniques, like polymerase chain reaction (PCR) techniques. This method is similar to the first aspect of the invention, but thermodynamically compatible oligonucleotides pairs or primers that hybridize to the same sequence at a distance which is within a given range are evaluated.
摘要翻译：本发明公开了一种可用于从复杂生物样品鉴定一个DNA序列或一个特定的DNA序列组的方法。不同的分子生物学方法需要使用称为寡核苷酸的短DNA序列，它们是从它们的组成基础的描述中人工合成的。所公开的方法允许设计可用于所述分子生物学过程的寡核苷酸，如探针设计程序，其特征在于构建参考序列数据库，选择属于靶生物的序列的子集，候选寡核苷酸的选择从这些序列中，根据杂交特异性和热力学稳定性标准，这些候选寡核苷酸的纯化，以及根据其分类学特异性对这些寡核苷酸的分选。在第二方面，公开了设计寡核苷酸对或引物的方法，其在某些分子生物学技术中是需要的，例如聚合酶链反应（PCR）技术。该方法类似于本发明的第一方面，但评价了在与给定范围内的距离处与相同序列杂交的热力学相容的寡核苷酸对或引物。

4. 发明授权

US11319612B2 Biotechnological procedure to remove magnetic sulfur impurities from iron concentrate ores 有权
公开(公告)号：US11319612B2
公开(公告)日：2022-05-03
申请号：US15755415
申请日：2016-08-24
申请人： BIOSIGMA S.A.
发明人： Roberto Andres Bobadilla Fazzini
IPC分类号： C22B3/18 , C22B1/11
摘要： Present invention describes a biotechnological procedure to remove magnetic sulfur impurities from iron concentrate, wherein includes: to bioleach iron concentrate ores agglomerated in heaps under temperature condition between 5 and 35° C., inoculating the iron concentrate ores with Acidithiobacillus thiooxidans cultures, with an inoculum concentration 104 and 106 cel/g and addition of water supplemented with nitrogen and phosphorous source (0.01 to 0.5 g (NH4)2HPO4/L), without potassium addition, adjusting pH between 1.0 and 9.0, and a feeding rate between 5 and 15 L/h/m2; this procedure allows a removal efficiency above 80% in 21 days, with a maximum iron loss of 3%.

5. 发明申请

US20140363879A1 SYSTEM AND METHOD FOR SULFUR BIOADAPTATION IN A STABLE DISPERSION FORM FOR INDUSTRIAL BIOREACTOR FEEDING 审中-公开
标题翻译：用于工业生物反应器输送的稳定分散形式中的硫生物处理系统和方法
公开(公告)号：US20140363879A1
公开(公告)日：2014-12-11
申请号：US14122541
申请日：2012-05-24
申请人： Pilar Parada Valdecantos , Sebastian Galvez Avendano , Ricardo Badilla Ohlbaum , Francisco Laurie Santander
发明人： Pilar Parada Valdecantos , Sebastian Galvez Avendano , Ricardo Badilla Ohlbaum , Francisco Laurie Santander
IPC分类号： C12N1/20
CPC分类号： C12N1/20 , C12N1/36
摘要： A system for sulfur (S) bioadaptation in a stable dispersion form for feeding industrial bioreactors, which are composed of a tank with a liquid medium made up of a supernatant dilution or part of a sulfur oxidizing bacterial culture, a recirculating pump fed from the tank, and an eductor which is connected at its input to the recirculation pump, and connected at its output back to the tank, closing the recirculation circuit. There is a supply of S in fine powder form present in the eductor's feeding path. Where the supernatant or part of a sulfur oxidizing bacterial culture is diluted between 100 and 10 times in sulfur containing culture medium. The second aspect the invention discloses a method for sulfur bioadaptation in a stable dispersion form for feeding industrial bioreactors, which includes initiating recirculation through a recirculation pump, in a tank which contains a liquid medium made up of a supernatant dilution or part of a sulfur oxidizing bacterial culture; connecting a supply of fine sulfur powder to an eductor, which is connected to the recirculation pump at its input, and its output connected back into the reservoir. The incorporated sulfur is pulverized by the suction produced within the eductor.
摘要翻译：一种用于供给工业生物反应器的稳定分散体形式的硫（S）生物适应系统，其由具有由上清液稀释物或部分硫氧化细菌培养物组成的液体培养基的罐组成，从罐中进料的循环泵以及喷射器，其在其输入处连接到再循环泵，并且在其输出处连接到罐，闭合再循环回路。在喷射器的喂养路径中存在细粉末形式的S供应。其中上清液或硫氧化细菌培养物的一部分在含硫培养基中稀释100至10倍。本发明的第二方面公开了一种用于供给工业生物反应器的稳定分散体形式的硫生物适应方法，其包括通过再循环泵启动再循环，所述罐包含由上清液稀释物或硫氧化物的一部分组成的液体介质细菌培养; 将精细硫磺粉末供应到喷射器，喷射器在其输入端连接到再循环泵，其输出连接回到储存器中。通过在喷射器内产生的吸力将掺入的硫粉碎。

6. 发明申请

US20110212527A1 METHOD TO INCREASE THE PRODUCTION OF EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) IN A ACIDITHIOBACILLUS FERROOXIDANS CULTURE BY THE INHIBITION OF ENZYMES OF TRICARBOXILIC ACID CYCLE 有权
标题翻译：通过抑制三羧酸循环酶的酶活性来增加酸性白僵菌培养物中细胞外聚合物（EPS）的生产方法
公开(公告)号：US20110212527A1
公开(公告)日：2011-09-01
申请号：US13035336
申请日：2011-02-25
申请人： Alejandro Eduardo Maass Sepúlveda , Pablo Andrés Moreno Cortéz , Marko Antonio Budinich Abarca , Pilar Angélica Parada Valdecantos , Leandro Mauricio Padilla Iglesias , Marlen Nayibe Barreto Roa
发明人： Alejandro Eduardo Maass Sepúlveda , Pablo Andrés Moreno Cortéz , Marko Antonio Budinich Abarca , Pilar Angélica Parada Valdecantos , Leandro Mauricio Padilla Iglesias , Marlen Nayibe Barreto Roa
IPC分类号： C12N15/00 , C12N9/99
CPC分类号： C12N1/38 , C12P1/04 , C12P19/04
摘要： A method of increasing the production of extracellular polymeric substances (EPS) in an Acidithiobacillus ferrooxidans culture is disclosed. The method includes inhibiting an enzyme, such as citrate synthase, aconitase, or isocitrate dehydrogenase, in the tricarboxylic acid (TCA) cycle leading to alpha-ketoglutarate.
摘要翻译：公开了一种增加酸性氧化硫杆菌培养物中细胞外聚合物质（EPS）产生的方法。该方法包括在导致α-酮戊二酸的三羧酸（TCA）循环中抑制酶，如柠檬酸合成酶，乌头酸酶或异柠檬酸脱氢酶。

7. 发明申请

US20110136125A1 METHOD FOR THE IDENTIFICATION AND QUANTIFICATION OF MICROORGANISMS USEFUL IN BIOMINING PROCESSES 有权
标题翻译：用于生物过程中有用的微生物鉴定和定量方法
公开(公告)号：US20110136125A1
公开(公告)日：2011-06-09
申请号：US12945407
申请日：2010-11-12
申请人： Pilar Angèlica Parada Valdecantos , Katia Nicole Ehrenfeld Stolzenbach , Igor Alejandro Pacheco Cruz , Alejandro Eduardo Maass Sepúlveda , Andrés Octavio Aravena Duarte , Mauricio Alejandro Gonzales Canales , Servet Martinez Aguilera
发明人： Pilar Angèlica Parada Valdecantos , Katia Nicole Ehrenfeld Stolzenbach , Igor Alejandro Pacheco Cruz , Alejandro Eduardo Maass Sepúlveda , Andrés Octavio Aravena Duarte , Mauricio Alejandro Gonzales Canales , Servet Martinez Aguilera
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C22B3/02 , C22B3/18 , Y02P10/234
摘要： The present invention discloses a method to identify and quantify environmental microorganisms useful in biomining processes. These microorganisms are basically 10, belonging to Bacteria: Acidiphilium sp., Leptospirillum sp., Sulfobacillus sp., Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans; and Archaea: Acidianus sp., Ferroplasma sp., Metallosphaera sp., Sulfolobus sp. and Thermoplasma sp.The method comprises performing a PCR with specific primers designed in our laboratories for different taxons SEQ ID No. 4 to SEQ ID No.: 407. With qPCR results and other data obtained from the analyzed sample, the microorganism concentration of each analyzed taxon present in the sample is calculated using a mathematical formula.
摘要翻译：本发明公开了一种鉴定和定量生物工艺中有用的环境微生物的方法。这些微生物基本上为10，属于细菌：嗜酸菌属，钩端螺旋体属，硫磺杆菌属，氧化亚铁硫杆菌和硫氧化硫酸硫杆菌; 和古菌：Acidianus sp。，Ferroplasma sp。，Metallosphaera sp。，Sulfolobus sp。和热血浆该方法包括在我们的实验室中针对SEQ ID No.4至SEQ ID No .: 407在不同分类群中设计的特异性引物进行PCR。使用qPCR结果和从分析的样品获得的其他数据，每个分析的分类群的微生物浓度存在于使用数学公式计算样本。

8. 发明申请

US20080127779A1 PROCESS TO INCREASE THE BIOLEACHING SPEED OF ORES OR CONCENTRATES OF SULFIDE METAL SPECIES, BY MEANS OF CONTINUOUS INOCULATION WITH LEACHING SOLUTION THAT CONTAINS ISOLATED MICROORGANISMS, WITH OR WITHOUT PRESENCE OF NATIVE MICROORGANISMS 有权
标题翻译：通过连续分析含有分离微生物的浸出溶液，或者不存在原生微生物的方法，提高硫化物金属物种的生物量或浓度的方法
公开(公告)号：US20080127779A1
公开(公告)日：2008-06-05
申请号：US11862035
申请日：2007-09-26
申请人： Pedro Antonio Morales Cerda , Ricardo Badilla Badilla Ohlbaum
发明人： Pedro Antonio Morales Cerda , Ricardo Badilla Badilla Ohlbaum
IPC分类号： C22B3/18
CPC分类号： C22B3/18 , C22B15/0071 , Y02P10/234 , Y02P10/236 , Y02P10/238
摘要： The invention publishes a process to increase the bioleaching speed of ores or concentrates of sulfide metal species in heaps, tailing dams, dumps, or other on-site operations. The process is characterized by the continuous inoculation of the ores or concentrates with isolated microorganisms of the Acidithiobacillus thiooxidans type, together with isolated microorganisms of the Acidithiobacillus ferrooxidans type, with or without native microorganisms, in such a way that the total concentration of microorganisms in the continuous inoculation flow is of around 1×107 cells/ml to 5,6×107 cells/ml. In particular, the invention publishes the continuous inoculation of Acidithiobacillus thiooxidans Licanantay DSM 17318 together with Acidithiobacillus ferrooxidans Wenelen DSM 16786 microorganisms, or with other native microorganisms at a concentration higher than 5×107 cells/ml. In addition to the inoculation of isolated bacteria, the invention includes the addition of oxidizing agents such as the ferric ion produced externally, together with nutrients in the shape of salts of ammonium, magnesium, iron, potassium, as well as air enriched continuously with carbon dioxide to promote bacterial action in the bioleaching process of ores or concentrates.
摘要翻译：本发明公开了一种增加堆积物，尾矿坝，垃圾场或其他现场操作中硫化物金属物质的矿石或精矿的生物浸出速度的方法。该方法的特征在于用硫酸氧化硫酸杆菌类型的分离的微生物连续接种矿石或浓缩物，以及具有或不具有天然微生物的分离的氧化亚硫酸氧化亚铁杆菌的微生物，使得微生物的总浓度连续接种流量约为1×10 7细胞/ ml至5×10 6细胞/ ml。特别地，本发明公开了硫酸氧化硫杆菌Licanantay DSM 17318与酸性氧化硫杆菌Wenelen DSM 16786微生物或其它天然微生物以高于5×10 7细胞/ ml的浓度的连续接种。除了接种分离的细菌外，本发明还包括加入氧化剂如外部产生的铁离子，以及铵，镁，铁，钾盐形式的营养物质，以及连续地用碳浓缩的空气二氧化物以促进矿石或精矿的生物浸出过程中的细菌作用。

9. 发明申请

US20070042482A1 Sulfur-oxidizing bacteria and their use in bioleaching processes for sulfured copper minerals 有权
标题翻译：硫氧化细菌及其在硫化铜矿物质生物浸出过程中的应用
公开(公告)号：US20070042482A1
公开(公告)日：2007-02-22
申请号：US11506031
申请日：2006-08-17
申请人： Atsuko Ohata , Manabu Manabe , Pilar Parada Valdecantos
发明人： Atsuko Ohata , Manabu Manabe , Pilar Parada Valdecantos
IPC分类号： C22B15/00 , C12P3/00 , C12N1/20
CPC分类号： C22B3/18 , C22B15/0063 , C22B15/0071 , Y02P10/234 , Y02P10/236
摘要： The present invention is related to an isolated chemolithotrophic bacterium belonging to species Acidithiobacillus thiooxidans named Licanantay, deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH-DSMZ with number DSM 17318, and its use in pure form or in mixtures that contains it for bioleaching processes of minerals or sulfured metallic species concentrates. This Licanantay strain DSM 17318 has sulfur-oxidizing activity in both primary and secondary sulfured minerals, especially in the case of chalcopyrite, covellite, bornite, chalcocite, enargite and tennantite.
摘要翻译：本发明涉及一种名为Licanantay的嗜酸硫杆菌硫代氧化物的分离的化学嗜热细菌，其存放在具有数字DSM 17318的Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH-DSMZ中，其用于纯形式或含有它的混合物，用于生物浸出过程矿物质或硫化金属物质浓缩物。这种Licanantay菌株DSM 17318在初级和次级硫化矿物中都具有硫氧化活性，特别是在黄铜矿，碳黑，斑铜矿，辉铜矿，榴辉岩和铁锰矿的情况下。

10. 发明授权

US08927246B2 Method to increase the production of extracellular polymeric substances (EPS) in a Acidithiobacillus ferrooxidans culture by the inhibition of enzymes of tricarboxilic acid cycle 有权
标题翻译：通过抑制三羧酸循环酶的方法增加酸性氧化硫杆菌培养物中细胞外聚合物质（EPS）的产生
公开(公告)号：US08927246B2
公开(公告)日：2015-01-06
申请号：US13035336
申请日：2011-02-25
申请人： Alejandro Eduardo Maass Sepúlved , Pablo Andrés Moreno Cortéz , Marko Antonio Budinich Abarca , Pilar Angélica Parada Valdecantos , Leandro Mauricio Padilla Iglesias , Marlen Nayibe Barreto Roa
发明人： Alejandro Eduardo Maass Sepúlved , Pablo Andrés Moreno Cortéz , Marko Antonio Budinich Abarca , Pilar Angélica Parada Valdecantos , Leandro Mauricio Padilla Iglesias , Marlen Nayibe Barreto Roa
IPC分类号： C12N9/99 , C12P1/04 , C12N1/38 , C12P19/04
CPC分类号： C12N1/38 , C12P1/04 , C12P19/04
摘要： A method of increasing the production of extracellular polymeric substances (EPS) in an Acidithiobacillus ferrooxidans culture is disclosed. The method includes inhibiting an enzyme, such as citrate synthase, aconitase, or isocitrate dehydrogenase, in the tricarboxylic acid (TCA) cycle leading to alpha-ketoglutarate.
摘要翻译：公开了一种增加酸性氧化硫杆菌培养物中细胞外聚合物质（EPS）产生的方法。该方法包括在导致α-酮戊二酸的三羧酸（TCA）循环中抑制酶，如柠檬酸合成酶，乌头酸酶或异柠檬酸脱氢酶。

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