专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09085801B2 Transposon end compositions and methods for modifying nucleic acids 有权
公开(公告)号：US09085801B2
公开(公告)日：2015-07-21
申请号：US13489204
申请日：2012-06-05
申请人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
发明人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
IPC分类号： C12Q1/68 , C12P19/34 , C12N15/10
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1093 , C12P19/34 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2535/122 , C12Q2521/507
摘要： Compositions of transposome complexes for generating DNA fragments with specific 5′- and 3′-tags. Kits for generating libraries for sequencing, with transposome complexes, enzymes, oligonucleotides or other components.

2. 发明授权

US09040256B2 Transposon end compositions and methods for modifying nucleic acids 有权
标题翻译：转座子末端组合物和修饰核酸的方法
公开(公告)号：US09040256B2
公开(公告)日：2015-05-26
申请号：US14148463
申请日：2014-01-06
申请人： Epicentre Technologies Corporation
发明人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
IPC分类号： C12Q1/68 , C12P19/34 , C12N15/10
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1093 , C12P19/34 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2535/122 , C12Q2521/507
摘要： The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next-generation sequencing.)
摘要翻译：本发明提供了使用转座酶和转座子末端在体外产生双链靶DNA的广泛断裂和5'标记的方法，组合物和试剂盒，然后使用DNA聚合酶产生5'-和3'标记单链DNA片段，而不进行PCR扩增反应，其中5'-末端的第一个标签显示转移的转座子末端的序列和任选的另外的任意序列，3'-末端的第二个标签显示出与第一个标签展示的序列不同的序列。该方法可用于产生用于各种过程的5'-和3'标记的DNA片段，包括环境样品中DNA的宏基因组分析过程，DNA拷贝数变异（CNV）分析和比较基因组测序（ CGS），包括大规模并行DNA测序（所谓的“下一代测序”）。

3. 发明授权

US07794971B1 Compositions and methods for controlling copy number for a broad range of plasmids and uses thereof 有权
标题翻译：用于控制多种质粒的拷贝数的组合物和方法及其用途
公开(公告)号：US07794971B1
公开(公告)日：2010-09-14
申请号：US10883459
申请日：2004-07-01
申请人： Darin J. Haskins , Leslie M. Hoffman
发明人： Darin J. Haskins , Leslie M. Hoffman
IPC分类号： C12N15/00 , C12N15/85
CPC分类号： C12N15/69
摘要： The present invention provides compositions and methods for controlling the copy number for a broad range of plasmids and uses thereof. Disclosed is a host cell for conditional control of copy number of a plasmid, which host cell comprises a poly(A) polymerase gene that is operably joined to a conditionally inducible promoter, and a method for cloning and stably maintaining a DNA sequence encoding a heterologous polypeptide in the host cell.
摘要翻译：本发明提供了用于控制大范围质粒的拷贝数的组合物和方法及其用途。公开了用于条件控制质粒拷贝数的宿主细胞，该宿主细胞包含可操作地连接到条件诱导型启动子的聚（A）聚合酶基因，以及用于克隆和稳定维持编码异源的DNA序列的方法多肽在宿主细胞中。

4. 发明申请

US20080293107A1 METHODS FOR USING RIBOPRIMERS FOR STRAND DISPLACEMENT REPLICATION OF TARGET SEQUENCES 有权
标题翻译：使用RIBOPRIMERS进行STRAND位移复制目标序列的方法
公开(公告)号：US20080293107A1
公开(公告)日：2008-11-27
申请号：US12186127
申请日：2008-08-05
申请人： Gary A. Dahl , Jerome J. Jendrisak , Agnes J. Radek
发明人： Gary A. Dahl , Jerome J. Jendrisak , Agnes J. Radek
IPC分类号： C12P19/34
CPC分类号： C12N15/1096 , C12Q1/6853 , C12Q2531/119 , C12Q2521/301 , C12Q2525/125
摘要： Methods, compositions and kits for amplifying a target sequence by strand displacement replication using strand-displacing primers. The method uses primers that have only ribonucleotides or purine ribonucleotides and at least one 2′-substituted pyrimidine-2′-deoxyribonucleotide.
摘要翻译：使用链置换引物通过链置换复制扩增靶序列的方法，组合物和试剂盒。该方法使用仅具有核糖核苷酸或嘌呤核糖核苷酸的引物和至少一个2'-取代的嘧啶-2'-脱氧核糖核苷酸。

5. 发明授权

US11421216B2 Nuclease-based RNA depletion 有权
公开(公告)号：US11421216B2
公开(公告)日：2022-08-23
申请号：US16721468
申请日：2019-12-19
申请人： EPICENTRE TECHNOLOGIES CORPORATION
发明人： Scott Kuersten , Frederick W. Hyde , Asako Tetsubayashi
IPC分类号： C12Q1/68 , C12N15/10 , C12Q1/6806 , C12Q1/6848 , C12Q1/6876
摘要： The present disclosure is related to methods and materials for depleting unwanted RNA species from a nucleic acid sample. In particular, the present disclosure describes how to remove unwanted rRNA, tRNA, mRNA or other RNA species that could interfere with the analysis, manipulation and study of target RNA molecules in a sample.

6. 发明授权

US11339422B2 Methods of analyzing nucleic acids 有权
公开(公告)号：US11339422B2
公开(公告)日：2022-05-24
申请号：US16830544
申请日：2020-03-26
申请人： EPICENTRE TECHNOLOGIES CORPORATION
发明人： Scott Kuersten , Agnes Radek , Ramesh Vaidyanathan , Haiying Li Grunenwald
IPC分类号： C12Q1/6806 , C12N15/10 , C12Q1/6874
摘要： Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).

7. 发明授权

US09963735B2 Selective 5′ ligation tagging of RNA 有权
公开(公告)号：US09963735B2
公开(公告)日：2018-05-08
申请号：US13647940
申请日：2012-10-09
申请人： Epicentre Technologies Corporation
发明人： Jerome Jendrisak , Gary A. Dahl , Ramesh Vaidyanathan
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1096 , C12Q1/6855 , C12Q2525/207 , C12Q2525/125 , C12Q2521/525
摘要： The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′ tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

8. 发明授权

US09080211B2 Transposon end compositions and methods for modifying nucleic acids 有权
标题翻译：转座子末端组合物和修饰核酸的方法
公开(公告)号：US09080211B2
公开(公告)日：2015-07-14
申请号：US12605337
申请日：2009-10-24
申请人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
发明人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
IPC分类号： C12Q1/68 , C12P19/34 , C12N15/10
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1093 , C12P19/34 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2535/122 , C12Q2521/507
摘要： The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next-generation sequencing.).
摘要翻译：本发明提供了使用转座酶和转座子末端在体外产生双链靶DNA的广泛断裂和5'标记的方法，组合物和试剂盒，然后使用DNA聚合酶产生5'-和3'标记单链DNA片段，而不进行PCR扩增反应，其中5'-末端的第一个标签显示转移的转座子末端的序列和任选的另外的任意序列，3'-末端的第二个标签显示出与第一个标签展示的序列不同的序列。该方法可用于产生用于各种过程的5'-和3'标记的DNA片段，包括环境样品中DNA的宏基因组分析过程，DNA拷贝数变异（CNV）分析和比较基因组测序（ CGS），包括大规模并行DNA测序（所谓的“下一代测序”）。

9. 发明申请

US20140179562A1 METHODS AND KITS FOR 3'-END-TAGGING OF RNA 有权
标题翻译： RNA的3'末端标记的方法和试剂盒
公开(公告)号：US20140179562A1
公开(公告)日：2014-06-26
申请号：US14026239
申请日：2013-09-13
申请人： Epicentre Technologies Corporation
发明人： Ramesh Vaidyanathan , Scott Kuersten , Ken Doyle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/10 , C12N15/1096 , C12N15/66 , C12P19/34
摘要： The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5′-activated, 3′-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.
摘要翻译：本发明提供了方法和试剂盒，其能够快速有效地双端标记RNA以制备用于通过诸如下一代RNA测序，qPCR，微阵列分析或克隆等应用进行分析的文库。该方法不需要本领域已知方法常见的耗时和低效的凝胶纯化步骤。此外，本发明提供了用于从标准的单链DNA寡核苷酸快速，高通量酶制备5'-活化的，3'-封闭的DNA寡核苷酸的方法和试剂盒。

10. 发明申请

US20110287435A1 TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS 有权
标题翻译： TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIZING NUCLEIC ACIDS
公开(公告)号：US20110287435A1
公开(公告)日：2011-11-24
申请号：US13177772
申请日：2011-07-07
申请人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
发明人： Haiying Li Grunenwald , Nicholas Caruccio , Jerome Jendrisak , Gary Dahl
IPC分类号： C12Q1/68 , C12N9/96
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1093 , C12P19/34 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2535/122 , C12Q2521/507
摘要： Compositions of transposome complexes for generating DNA fragments with specific 5′- and 3′-tags. Kits for generating libraries for sequencing, with transposome complexes, enzymes, oligonucleotides or other components.
摘要翻译：用于产生具有特异性5'-和3'标签的DNA片段的转座子复合物的组合物。用于产生用于测序的文库的试剂盒，具有转座体复合物，酶，寡核苷酸或其它组分。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式