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1. 发明申请

US20130130932A1 Method for Identifying Lineage-Related Antibodies 有权
标题翻译：鉴定谱系相关抗体的方法
公开(公告)号：US20130130932A1
公开(公告)日：2013-05-23
申请号：US13748507
申请日：2013-01-23
申请人： Epitomics, Inc.
发明人： GUO-LIANG YU
IPC分类号： G01N33/68
CPC分类号： G01N33/577 , C07K16/00 , C07K16/2863 , C07K2317/10 , C07K2317/56 , C07K2317/76 , C12Q1/6876 , G01N33/566 , G01N33/6854
摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.
摘要翻译：在某些实施方案中，该方法可以包括：a）从B细胞群获得抗体序列; b）将抗体序列分组以提供多组谱系相关抗体; c）在生物测定中测试来自每个组的单一抗体，并且在鉴定了第一抗体后，d）在第二生物测定中测试与第一抗体在同一组中的另外的抗体。在另一个实施方案中，该方法可以包括：a）测试从动物的产生抗体的器官的第一部分获得的多个抗体; b）获得第一鉴定抗体的序列; c）从所述抗体产生器官的第二部分获得与所述第一抗体谱系相关的其它抗体的序列; 和c）在第二生物测定中测试另外的抗体。

2. 发明授权

US08367408B2 Fusion partner for production of monoclonal rabbit antibodies 有权
标题翻译：用于生产单克隆兔抗体的融合伙伴
公开(公告)号：US08367408B2
公开(公告)日：2013-02-05
申请号：US13274946
申请日：2011-10-17
申请人： Robert Pytela , Weimin Zhu , Yaohuang Ke , Qi Qian , Harry C. Au
发明人： Robert Pytela , Weimin Zhu , Yaohuang Ke , Qi Qian , Harry C. Au
IPC分类号： C12N5/20
CPC分类号： C07K16/00 , C07K2317/24 , C07K2317/622 , C12N5/163 , C12P21/005
摘要： The invention provides a rabbit-derived immortal B-lymphocyte capable of fusion with a rabbit splenocyte to produce a hybrid cell that produces an antibody. The immortal B-lymphocyte does not detectably express endogenous immunoglobulin heavy chain and may contain, in certain embodiments, an altered immunoglobulin heavy chain-encoding gene. A hybridoma resulting from fusion between the subject immortal B-lymphocyte and a rabbit antibody-producing cell is provided, as is a method of using that hybridoma to produce an antibody. The subject invention finds use in a variety of different diagnostic, therapeutic and research applications.
摘要翻译：本发明提供能够与兔脾细胞融合以产生产生抗体的杂交细胞的兔衍生的永生性B淋巴细胞。不朽的B淋巴细胞不可检测地表达内源性免疫球蛋白重链，并且在某些实施方案中可能含有改变的免疫球蛋白重链编码基因。提供了由受试者不朽B淋巴细胞和产生兔抗体的细胞融合而产生的杂交瘤，以及使用该杂交瘤产生抗体的方法。本发明可用于各种不同的诊断，治疗和研究应用。

3. 发明申请

US20050287118A1 Bacterial plasmid with immunological adjuvant function and uses thereof 审中-公开
标题翻译：具有免疫佐剂功能的细菌质粒及其用途
公开(公告)号：US20050287118A1
公开(公告)日：2005-12-29
申请号：US10997747
申请日：2004-11-23
申请人： Maoxin Tian , William Rutter , Mark Selby
发明人： Maoxin Tian , William Rutter , Mark Selby
IPC分类号： A61K39/00 , A61K39/39 , A61K48/00 , C07H21/04 , C07K16/18 , C12N5/06 , C12P21/04 , C12P21/06
CPC分类号： A61K39/0011 , A61K39/39 , A61K2039/53 , A61K2039/55522 , A61K2039/55527 , A61K2039/55533 , A61K2039/55538 , A61K2039/55561 , A61K2039/57
摘要： Plasmid adjuvant compositions and methods for enhancing an immune response to a coadministered immunogen are described. The plasmid adjuvants include a combination of cytokines and chemokines designed to elicit an enhanced immune response. Particular combinations can be provided to generate a Th1 and/or a Th2 immune response.
摘要翻译：描述了用于增强对共同给药的免疫原的免疫应答的质粒佐剂组合物和方法。质粒佐剂包括细胞因子和趋化因子的组合，其被设计用于引发增强的免疫应答。可以提供特定的组合以产生Th1和/或Th2免疫应答。

4. 发明授权

US09696314B2 Method for identifying lineage-related antibodies 有权
公开(公告)号：US09696314B2
公开(公告)日：2017-07-04
申请号：US14973523
申请日：2015-12-17
申请人： Epitomics, Inc.
发明人： Guo-Liang Yu
IPC分类号： C07K16/00 , G01N33/53 , G01N33/577 , C07K16/28 , G01N33/68 , G01N33/566 , C12Q1/68
CPC分类号： G01N33/577 , C07K16/00 , C07K16/2863 , C07K2317/10 , C07K2317/56 , C07K2317/76 , C12Q1/6876 , G01N33/566 , G01N33/6854
摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.

5. 发明授权

US09250244B2 Method for identifying lineage-related antibodies 有权
公开(公告)号：US09250244B2
公开(公告)日：2016-02-02
申请号：US14183075
申请日：2014-02-18
申请人： Epitomics, Inc. (c/o Abcam plc)
发明人： Guo-Liang Yu
IPC分类号： C07K16/00 , G01N33/53 , G01N33/577 , C07K16/28 , G01N33/68 , G01N33/566
CPC分类号： G01N33/577 , C07K16/00 , C07K16/2863 , C07K2317/10 , C07K2317/56 , C07K2317/76 , C12Q1/6876 , G01N33/566 , G01N33/6854
摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.

6. 发明授权

US09057728B2 FACS-based method for obtaining an antibody sequence 有权
标题翻译：用于获得抗体序列的基于FACS的方法
公开(公告)号：US09057728B2
公开(公告)日：2015-06-16
申请号：US13545857
申请日：2012-07-10
申请人： Yaohuang Ke , Guo-Liang Yu , Karin Vroom
发明人： Yaohuang Ke , Guo-Liang Yu , Karin Vroom
IPC分类号： G01N33/564 , G01N33/569 , G01N33/68 , G01N33/50
CPC分类号： G01N33/56972 , G01N33/5032 , G01N33/5047 , G01N33/564 , G01N33/6854
摘要： In certain embodiments, the method may comprises: a) contacting a population of permeabilized, cross-linked antibody-producing cells with a labeled antigen to produce a labeled sample in which cells that produce an antibody that specifically binds to said antigen are intracellularly labeled; b) using FACS to isolate cells that are intracellularly labeled, thereby producing labeled cells; c) uncrosslinking said labeled cells to produce uncrosslinked cells; and d) amplifying heavy and light chain-encoding nucleic acid from individual uncrosslinked cells, thereby obtaining nucleic acid that encodes the variable domain of antibody that specifically binds to said antigen.
摘要翻译：在某些实施方案中，所述方法可以包括：a）将一组透化的，交联的产生抗体的细胞与标记的抗原接触以产生标记的样品，其中产生与所述抗原特异性结合的抗体的细胞是细胞内标记的; b）使用FACS分离细胞内标记的细胞，从而产生标记细胞; c）未交联所述标记的细胞以产生未交联的细胞; 和d）从单个未交联的细胞扩增重链和轻链编码核酸，从而获得编码与所述抗原特异性结合的抗体可变结构域的核酸。

7. 发明申请

US20140179556A1 Method for Identifying Lineage-Related Antibodies 有权
公开(公告)号：US20140179556A1
公开(公告)日：2014-06-26
申请号：US14183075
申请日：2014-02-18
申请人： Epitomics, Inc. (c/o Abcam plc)
发明人： GUO-LIANG YU
IPC分类号： G01N33/577
CPC分类号： G01N33/577 , C07K16/00 , C07K16/2863 , C07K2317/10 , C07K2317/56 , C07K2317/76 , C12Q1/6876 , G01N33/566 , G01N33/6854
摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.

8. 发明授权

US07462697B2 Methods for antibody engineering 有权
标题翻译：抗体工程方法
公开(公告)号：US07462697B2
公开(公告)日：2008-12-09
申请号：US10984473
申请日：2004-11-08
申请人： Fernando Jose Rebelo Do Couto , Kristin B. Hendricks , S. Ellen Wallace , Guo-Liang Yu
发明人： Fernando Jose Rebelo Do Couto , Kristin B. Hendricks , S. Ellen Wallace , Guo-Liang Yu
IPC分类号： C12P21/08 , A61K39/00 , G01N33/53
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
摘要翻译：本发明提供了鉴定抗体的位置的方法，该方法可以在不显着降低抗体结合活性的情况下进行修饰。在许多实施方案中，该方法包括通过将其氨基酸序列与多个与亲本抗体相同的抗原结合的多种相关抗体的氨基酸序列进行比较来鉴定亲本抗体中的可取代位置。可取代位置的氨基酸可以代替不同的氨基酸而不显着影响抗体的活性。可以使用本发明的方法来改变CDR的氨基酸序列，而不显着降低抗体的抗体在人源化方法或其它抗体工程方法中的亲和力。本发明可用于各种治疗，诊断和研究应用。

9. 发明授权

US07431927B2 TNFα-neutralizing antibodies 有权
标题翻译： TNFα中和抗体
公开(公告)号：US07431927B2
公开(公告)日：2008-10-07
申请号：US11090105
申请日：2005-03-24
申请人： Fernando Jose Rebelo Do Couto , Kristin Beth Hendricks , Stacey Ellen Wallace
发明人： Fernando Jose Rebelo Do Couto , Kristin Beth Hendricks , Stacey Ellen Wallace
IPC分类号： A61K39/395 , C07K16/24 , C12P21/08 , C12N1/00 , C12N1/16 , C12N15/00
CPC分类号： C07K16/241 , C07K2317/20
摘要： The invention provides monoclonal antibodies that neutralize TNFα activity. The monoclonal antibodies may be rabbit monoclonal antibodies or monoclonal antibodies having CDR regions derived from those rabbit monoclonal antibodies. In certain embodiments, the monoclonal antibodies may be humanized. Methods of using the subject antibodies to inhibit TNFα activity, methods of treatment using those antibodies and kits containing the same are also provided. The invention finds use in a variety of research and medical applications.
摘要翻译：本发明提供中和TNFα活性的单克隆抗体。单克隆抗体可以是具有衍生自那些兔单克隆抗体的CDR区的兔单克隆抗体或单克隆抗体。在某些实施方案中，单克隆抗体可以被人源化。还提供了使用受试者抗体抑制TNFα活性的方法，使用这些抗体的治疗方法和含有该抗体的试剂盒。本发明可用于各种研究和医学应用。

10. 发明授权

US07402409B2 Cell fusion method 有权
标题翻译：细胞融合法
公开(公告)号：US07402409B2
公开(公告)日：2008-07-22
申请号：US10350841
申请日：2003-01-23
申请人： Guo-Liang Yu
发明人： Guo-Liang Yu
IPC分类号： C12P21/08 , C12N5/06 , C12N5/16 , C07H21/04
CPC分类号： C12N5/163
摘要： The invention provides methods for fusing a first cell with a second cell to form a hybrid cell. The methods involve incubating a first parental cell producing a first partner of a fusogenic binding partner pair on its surface with a second parental cell producing a second partner of the fusogenic binding partner pair on its surface. In certain embodiments, the parental cells are incubated with a known fusogen such as polyethylene glycol and the fusogenic binding partner pair increases the rate of cell fusion. In many embodiments, the first cell is an antibody producing cell, the second cell is an immortal cell, and the hybrid cell is a hybridoma cell that produces a monoclonal antibody. Also provided by the invention are methods for producing hybridoma cells, and methods for screening those cells for production of a monoclonal antibody of interest. The invention further provides systems and kits for carrying out the subject methods. The subject methods, systems, and kits find use in a variety of different industrial, medical and research applications.
摘要翻译：本发明提供了将第一细胞与第二细胞融合以形成杂交细胞的方法。所述方法包括孵育第一亲本细胞，其第一亲代细胞在其表面上与第二亲本细胞产生融合结合配偶体对的第一个配偶体，从而产生其表面上融合结合配偶体对的第二个配偶体。在某些实施方案中，将母体细胞与已知的融合蛋白如聚乙二醇一起孵育，并且融合结合配偶体对增加细胞融合的速率。在许多实施方案中，第一细胞是产生抗体的细胞，第二细胞是永生细胞，杂交细胞是产生单克隆抗体的杂交瘤细胞。本发明还提供了用于产生杂交瘤细胞的方法，以及用于筛选用于产生感兴趣的单克隆抗体的细胞的方法。本发明还提供了用于实施本发明方法的系统和试剂盒。主题方法，系统和试剂盒可用于各种不同的工业，医学和研究应用。

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