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1. 发明授权

US08592179B2 Artificial binding proteins based on a modified alpha helical region of ubiquitin 有权
标题翻译：基于泛素修饰的α螺旋区的人工结合蛋白
公开(公告)号：US08592179B2
公开(公告)日：2013-11-26
申请号：US12514550
申请日：2007-11-15
申请人： Michael Schraeml , Erik Fiedler
发明人： Michael Schraeml , Erik Fiedler
IPC分类号： C07K14/435
CPC分类号： C07K14/00 , G01N33/6803
摘要： The present invention is directed to a method for the generation of binding proteins derived from the protein super-family of ubiquitin like proteins with modifications in their alpha helical region as well as to a protein obtainable by said method. Furthermore, the invention provides the use of a protein for the specific recognition, binding and neutralization of a predescribed target molecule, for the detection, quantitative determination, separation and/or for the isolation of a corresponding binding partner and the use of a protein of the invention, for diagnosis, prophylaxis and treatment of diseases in which the corresponding binding partner is directly or indirectly involved.
摘要翻译：本发明涉及一种用于产生衍生自泛蛋白样蛋白质的蛋白质超家族的结合蛋白质的方法，其修饰在其α螺旋区域以及可通过所述方法获得的蛋白质。此外，本发明提供蛋白质用于预测的靶分子的特异性识别，结合和中和用于检测，定量测定，分离和/或分离相应结合配偶体的用途以及使用蛋白质本发明用于诊断，预防和治疗其中相应的结合伴侣直接或间接参与的疾病。

2. 发明申请

US20130052715A1 METHOD FOR PRODUCING RECOMBINANT THROMBIN 审中-公开
标题翻译：生产重组血红蛋白的方法
公开(公告)号：US20130052715A1
公开(公告)日：2013-02-28
申请号：US13641844
申请日：2011-04-20
申请人： Andreas Anton , Arndt Dietrich , Jochen Koetter , Joerg Schaeffner
发明人： Andreas Anton , Arndt Dietrich , Jochen Koetter , Joerg Schaeffner
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： The invention relates to a method for producing folded prethrombin, wherein inclusion bodies, which contain unfolded prethrombin or a derivative thereof, are solubilized in a solubilization buffer containing at least one chaotropic compound and at least one organic disulfide compound. The invention further relates to methods for producing thrombin and a-thrombin and derivatives thereof. The invention also relates to solutions that contain folded proteins, which can be produced by the methods according to the invention.
摘要翻译：本发明涉及一种生产折叠凝血酶原的方法，其中包含未折叠凝血酶原或其衍生物的包涵体溶解于含有至少一种离液序列化合物和至少一种有机二硫化物的增溶缓冲液中。本发明还涉及产生凝血酶和α-凝血酶及其衍生物的方法。本发明还涉及可以通过根据本发明的方法生产的折叠蛋白质的溶液。

3. 发明授权

US08791238B2 Artificial binding proteins based on ubiquitin 有权
标题翻译：基于泛素的人工结合蛋白
公开(公告)号：US08791238B2
公开(公告)日：2014-07-29
申请号：US12072959
申请日：2008-02-29
申请人： Markus Fiedler , Ulrike Fiedler , Rainer Rudolph
发明人： Markus Fiedler , Ulrike Fiedler , Rainer Rudolph
IPC分类号： A61K38/00 , C12N15/10 , C07K14/00 , C40B40/02 , G01N33/68
CPC分类号： C12N15/1044 , C07K14/00 , C07K2319/00 , C12N15/1037 , C40B40/02 , G01N33/6872
摘要： The invention relates to modified proteins of the superfamily of “ubiquitin-like proteins”, proteins that have a ubiquitin-like fold and fragments or fusion proteins thereof. As a result of said modification, the proteins have a binding affinity with respect to a predetermined binding partner that did not exist previously. The invention also relates to a method for the production and utilization of said proteins.
摘要翻译：本发明涉及“遍在蛋白样蛋白”的超家族的修饰蛋白质，具有泛素样折叠的蛋白质及其片段或融合蛋白质。作为所述修饰的结果，蛋白质相对于先前不存在的预定结合配偶体具有结合亲合力。本发明还涉及生产和利用所述蛋白质的方法。

4. 发明授权

US08592144B2 Beta-sheet proteins with specific binding properties 有权
标题翻译：具有特异性结合特性的β-折叠蛋白
公开(公告)号：US08592144B2
公开(公告)日：2013-11-26
申请号：US11656646
申请日：2007-01-23
申请人： Ulrike Fiedler , Rainer Rudolph
发明人： Ulrike Fiedler , Rainer Rudolph
IPC分类号： C12Q1/68
CPC分类号： C07K14/00 , C07K14/435 , C07K14/47 , Y02A90/26
摘要： The present invention describes novel beta-sheet proteins having specific binding properties and catalytic properties and also methods for preparing these proteins.
摘要翻译：本发明描述了具有特异性结合特性和催化性质的新型β-折叠蛋白以及制备这些蛋白质的方法。

5. 发明申请

US20120015819A1 MIXTURE OF AT LEAST TWO FUSION PROTEINS, THE PRODUCTION THEREOF AND THE USE OF THE SAME 有权
标题翻译：至少两种融合蛋白的混合物，其生产及其使用
公开(公告)号：US20120015819A1
公开(公告)日：2012-01-19
申请号：US13222272
申请日：2011-08-31
申请人： Matthias PASCHKE
发明人： Matthias PASCHKE
IPC分类号： C40B10/00 , C40B30/04
CPC分类号： C40B30/04 , C07K2319/10 , C07K2319/60 , C07K2319/73 , C07K2319/81 , C12N15/1037 , C12N15/62 , C40B40/02 , G01N2500/04 , G01N2500/10
摘要： The present invention concerns a protein mixture comprising at least a first fusion protein comprising a protein or protein fragment, and an interaction domain and a protein translocation sequence, which effects that the fusion protein upon expression in a bacterium is translocated through the cytoplasmic membrane in an essentially unfolded state and at least a second fusion protein comprising a protein or protein fragment, and an interaction domain and a protein translocation sequence which effects that the fusion protein is translocated through the cytoplasmic membrane upon expression in a bacterium in an essentially folded state, wherein the interaction domain of the first protein can bind to those of the second protein.
摘要翻译：本发明涉及包含至少包含蛋白质或蛋白质片段的第一融合蛋白以及相互作用结构域和蛋白质易位序列的蛋白质混合物，所述互作用结构域和蛋白质易位序列影响在细菌中表达时的融合蛋白通过细胞质膜被转位基本上解折叠状态，以及至少第二融合蛋白，其包含蛋白质或蛋白质片段，以及相互作用结构域和蛋白质易位序列，其在基本折叠状态下在细菌中表达时影响融合蛋白被转位穿过细胞质膜，其中第一蛋白质的相互作用结构域可以结合第二蛋白质的相互作用结构域。

6. 发明申请

US20070111287A1 Design of beta-sheet proteins with specific binding properties 有权
标题翻译：具有特异性结合特性的β-折叠蛋白的设计
公开(公告)号：US20070111287A1
公开(公告)日：2007-05-17
申请号：US11656646
申请日：2007-01-23
申请人： Ulrike Fiedler , Rainer Rudolph
发明人： Ulrike Fiedler , Rainer Rudolph
IPC分类号： G06F19/00 , C07H21/04 , C12P21/06
CPC分类号： C07K14/00 , C07K14/435 , C07K14/47 , Y02A90/26
摘要： The present invention describes novel beta-sheet proteins having specific binding properties and catalytic properties and also methods for preparing these proteins.
摘要翻译：本发明描述了具有特异性结合特性和催化性质的新型β-折叠蛋白以及制备这些蛋白质的方法。

7. 发明授权

US08790895B2 Generation of artificial binding proteins on the basis of ubiquitin proteins 有权
标题翻译：在泛素蛋白的基础上生成人工结合蛋白
公开(公告)号：US08790895B2
公开(公告)日：2014-07-29
申请号：US11283332
申请日：2005-11-18
申请人： Markus Fiedler , Ulrike Fiedler , Rainer Rudolph
发明人： Markus Fiedler , Ulrike Fiedler , Rainer Rudolph
IPC分类号： A61K38/00 , C12N15/10 , G01N33/68 , C40B40/02 , C07K14/00
CPC分类号： C12N15/1044 , C07K14/00 , C07K2319/00 , C12N15/1037 , C40B40/02 , G01N33/6872
摘要： The invention relates to modified proteins of the superfamily of “ubiquitin-like proteins”, proteins that have a ubiquitin-like fold and fragments or fusion proteins thereof. As a result of said modification, the proteins have a binding affinity with respect to a predetermined binding partner that did not exist previously. The invention also relates to a method for the production and utilization of said proteins.
摘要翻译：本发明涉及“遍在蛋白样蛋白”的超家族的修饰蛋白质，具有泛素样折叠的蛋白质及其片段或融合蛋白质。作为所述修饰的结果，蛋白质相对于先前不存在的预定结合配偶体具有结合亲合力。本发明还涉及生产和利用所述蛋白质的方法。

8. 发明授权

US08501439B2 Method for obtaining active pro-NGF and beta-NGF 有权
标题翻译：获得活性前NGF和β-NGF的方法
公开(公告)号：US08501439B2
公开(公告)日：2013-08-06
申请号：US12765229
申请日：2010-04-22
申请人： Anke Rattenholl , Adelbert Grossmann , Elisabeth Schwarz , Rainer Rudolph
发明人： Anke Rattenholl , Adelbert Grossmann , Elisabeth Schwarz , Rainer Rudolph
IPC分类号： C12N15/18
CPC分类号： C07K14/48
摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.
摘要翻译：本发明涉及从前体proNGF生产生物活性β-NGF的方法。在原核宿主细胞中表达β-NGF的形式后，以不溶性无活性聚集体（包涵体）的形式分离重组蛋白。在将其溶解在强变性剂中并随后转化成通过存在于天然β-NGF中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性β-NGF。

9. 发明申请

US20120301393A1 MODIFIED UBIQUITIN PROTEINS HAVING A SPECIFIC BINDING ACTIVITY FOR THE EXTRADOMAIN B OF FIBRONECTIN 审中-公开
标题翻译：具有特异性结合活性的修饰的UBIQUITIN蛋白对纤维蛋白的超量B
公开(公告)号：US20120301393A1
公开(公告)日：2012-11-29
申请号：US13516002
申请日：2010-12-14
申请人： Arnd Steuernagel , Erik Fiedler , Markus Fiedler , Anja Kunert , Joerg Nerkamp , Thomas Goettler , Manja Gloser , Ilka Haenssgen
发明人： Arnd Steuernagel , Erik Fiedler , Markus Fiedler , Anja Kunert , Joerg Nerkamp , Thomas Goettler , Manja Gloser , Ilka Haenssgen
IPC分类号： A61K38/16 , C12N9/96 , A61K33/24 , G01N33/53 , C12N15/62 , C12N15/63 , C12N1/21 , A61P35/00 , C07K14/00 , A61K51/00
CPC分类号： C12N15/1034 , C07K14/435 , C07K14/47 , C07K14/525 , C07K2319/31 , C07K2319/95 , C12N15/1037 , C12N15/1041 , C12N15/1044 , G01N33/6845 , G01N33/6878
摘要： The present invention refers to novel recombinant proteins obtained from modified ubiquitin capable of binding the extradomain B of fibronectin (ED-B). Furthermore, the invention refers to fusion proteins comprising said recombinant protein fused to a pharmaceutically and/or diagnostically active component.
摘要翻译：本发明涉及从能够结合纤连蛋白的外部区域B（ED-B）的修饰的泛素得到的新型重组蛋白质。此外，本发明涉及包含与药学和/或诊断活性成分融合的所述重组蛋白的融合蛋白。

10. 发明授权

US08318671B1 Pharmaceutical preparations comprising human proNGF 有权
标题翻译：包含人类proNGF的药物制剂
公开(公告)号：US08318671B1
公开(公告)日：2012-11-27
申请号：US09807096
申请日：1999-10-11
申请人： Anke Rattenholl , Adelbert Grossmann , Elisabeth Schwarz , Rainer Rudolph
发明人： Anke Rattenholl , Adelbert Grossmann , Elisabeth Schwarz , Rainer Rudolph
IPC分类号： C07K14/48 , A61K38/18
CPC分类号： C07K14/48
摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.
摘要翻译：本发明涉及从前体proNGF生产生物活性的NGF的方法。在原核宿主细胞中表达生物素-NGF的形式后，重组蛋白以不溶性非活性聚集体（包涵体）的形式分离。在将其溶解在强变性剂中并随后转化成通过存在于天然和/或生长因子中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性。

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