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1. 发明公开

US20240199690A1 METHOD FOR PRODUCING PEPTONES COMPRISING PROTEINS AND AMINO ACIDS FROM MUCOUS MEMBRANES OF ANIMALS TO BE SLAUGHTERED, AND THE PEPTONES THEMSELVES 审中-公开
公开(公告)号：US20240199690A1
公开(公告)日：2024-06-20
申请号：US18555048
申请日：2022-02-18
申请人： CREMER OLEO GmbH & Co. KG
发明人： Steffen MAINZER , Heinrich KLEINE KLAUSING
IPC分类号： C07K1/34 , C07K1/30
CPC分类号： C07K1/34 , C07K1/306
摘要： A method having the following steps: (1) providing a composition containing proteins and amino acids from mucous membranes of animals for slaughter, inorganic salts and water, wherein the sum of the mass of proteins and amino acids in the dry mass of said composition is 30 to 70 weight percent and the mass of inorganic salts in the dry mass of said composition is at least 7.5 weight percent; (2) filtering the composition to obtain a filtrate and a filter cake; (3) cooling the filtrate to a temperature in the range of 15° C. to 15° C. to form a precipitate; (4) separating the resulting precipitate to obtain the liquid phase as product. The disclosure further relates to the products of this method.

2. 发明公开

US20240183865A1 METHOD FOR MEASURING AGGREGATING PROTEINS, METHOD FOR VISUALIZING AGGREGATION, AND KIT USED THEREFOR 审中-公开
公开(公告)号：US20240183865A1
公开(公告)日：2024-06-06
申请号：US18285090
申请日：2022-03-31
申请人： KANEKA CORPORATION , MURORAN INSTITUTE OF TECHNOLOGY
发明人： Naoki NISHISHITA , Akira KOBAYASHI , Kiyotaka TOKURAKU , Koji UWAI , Masahiro KURAGANO
IPC分类号： G01N33/68 , C07K1/34 , G01N33/58
CPC分类号： G01N33/6896 , C07K1/34 , G01N33/58 , G01N2333/4712 , G01N2333/4713 , G01N2333/76
摘要： The objective of the present invention is to provide a method for visualizing an aggregate of aggregating proteins in an environment similar to in vivo conditions. More specifically, the present invention relates to a method for collecting an aggregating protein-containing solution by allowing an aggregating protein-containing solution to pass through a filtration filter through which a solution for accelerating and visualizing protein aggregation has passed; a method for visualizing an aggregate of aggregating proteins comprising visualizing an aggregate of aggregating proteins in the aggregating protein-containing solution collected by the collecting method; and a method of forming an aggregate of labeled aggregating proteins of interest in a medium or culture supernatant from which an aggregation inhibitor of 40 kDa or higher has been removed, so as to visualize aggregation.

3. 发明公开

US20240101597A1 SEPARATION APPARATUS AND METHOD 审中-公开
公开(公告)号：US20240101597A1
公开(公告)日：2024-03-28
申请号：US18262989
申请日：2021-08-30
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： Andrew Lawrence Zydney , Christopher J. Yehl
IPC分类号： C07K1/34 , B01D61/16 , B01D61/18 , B01D63/02 , C07K14/765 , C07K14/805 , C07K16/28
CPC分类号： C07K1/34 , B01D61/16 , B01D61/18 , B01D63/02 , C07K14/765 , C07K14/805 , C07K16/2803
摘要： Protein separation can be provided based on a High Performance Countercurrent Membrane Purification (HPCMP), which can exploit highly selective diffusive transport across thin walls of a hollow fiber membrane for separation of proteins. Embodiments of an HPCMP system can separate mixtures of multiple proteins (e.g. separate BSA and Mb) or other biological material and provide high yields (e.g. achieving greater than 98% yield of both proteins with purification factors greater than 100-fold, etc.). Embodiments of a HPCMP system can be configured for high resolution separations in the preparation of biopharmaceuticals and natural protein products. Other embodiments can be utilized in other protein separation environments or biological material separation environments.

4. 发明公开

US20240099954A1 HEMP PEPTIDE COMPOSITIONS FOR NUTRACEUTICAL AND PERSONAL CARE PRODUCTS 审中-公开
公开(公告)号：US20240099954A1
公开(公告)日：2024-03-28
申请号：US17767627
申请日：2020-03-23
申请人： EC LABS INC.
发明人： Louis HEERZE
IPC分类号： A61K8/64 , A61Q5/00 , A61Q19/00 , C07K1/34
CPC分类号： A61K8/645 , A61Q5/00 , A61Q19/00 , C07K1/34
摘要： A skin and/or hair care composition includes a mixture of low-molecular weight hemp (LMWH) peptides, some or all of the peptides are less than about 1 kDa in size. The composition may be a nutraceutical, food product, beverage or topical formulation. The peptides may be obtained by enzymatic hydrolysis of hemp proteins. The composition may be used to repair, mitigate or improve skin or hair health or conditions.

5. 发明公开

US20240083980A1 METHOD TO IMPROVE VIRUS FILTRATION CAPACITY 审中-公开
公开(公告)号：US20240083980A1
公开(公告)日：2024-03-14
申请号：US18298233
申请日：2023-11-21
申请人： Genentech, Inc.
发明人： Amit MEHTA
IPC分类号： C07K16/06 , C07K1/18 , C07K1/34 , C07K1/36
CPC分类号： C07K16/065 , C07K1/18 , C07K1/34 , C07K1/36
摘要： The present invention relates to the field of protein purification. In particular, the invention concerns methods for increasing the filtration capacity of virus filters, by combined use of endotoxin removal and cation-exchange media in the prefiltration process.

6. 发明公开

US20240049745A1 PROTEIN ISOLATE 审中-公开
公开(公告)号：US20240049745A1
公开(公告)日：2024-02-15
申请号：US18493456
申请日：2023-10-24
申请人： AVRIL , UNIVERSITE DE LORRAINE , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
发明人： Claire DEFAIX , Romain KAPEL , Olivier GALET
IPC分类号： A23J1/14 , C07K1/34
CPC分类号： A23J1/142 , A23J1/148 , C07K1/34 , C07K14/76
摘要： A process for producing a protein isolate from an oilseed meal, and the isolate thus obtained, said isolate comprising proteins and an amount of 4 wt % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate. The process may comprise the following steps: a) providing an oilseed meal; b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase; c) separating said solid phase from said slurry, d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, preferably from 2 to 3, to form a mixture said mixture having a liquid phase; e) separating said liquid phase from said mixture formed in step d); f) f1) mixing the separated liquid phase to a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase; and/or f2) mixing the separated liquid to a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05 M to 0.5 M, at a temperature ranging from 40° C. to 70° C., to obtain a mixture having a liquid phase and a solid phase; g) precipitating a solid phase from the liquid of step f) for example by a cooling down step of the mixture to a temperature of 30° C. or less; h) separating said solid precipitate from the liquid of step g) said liquid comprising a water-rich liquid phase and an oil-rich liquid phase; i) separating said water-rich liquid phase from said oil-rich liquid phase, j) subjecting said water-rich liquid phase obtained in step i) to one or several membrane filtration(s) to obtain a protein isolate; and k) optionally, drying said protein isolate to obtain a dry protein isolate.

7. 发明公开

US20240043469A1 MANUFACTURE OF ENDOTOXIN-FREE HEMOGLOBIN-BASED DRUG SUBSTANCE AND METHOD FOR ENDOTOXIN-FREE PROTEIN PURIFICATION 审中-公开
公开(公告)号：US20240043469A1
公开(公告)日：2024-02-08
申请号：US18115724
申请日：2023-02-28
申请人： Medical Technology Associates II, Inc.
发明人： Carl W. Rausch
IPC分类号： C07K1/34 , C07K1/16 , C07K14/805
CPC分类号： C07K1/34 , C07K1/16 , C07K14/805 , B01D69/08
摘要： The present invention relates to the surprising discovery that previous hemoglobin-based drug purification methodologies do not remove sufficient endotoxins exposures at the various steps which may complex with the hemoglobin protein. These complexed endotoxins can result in serious health complications (e.g. development of cardiac lesions for one). Additionally, varied endotoxin types and concentration contributes to batch-to-batch variability during hemoglobin-based drug manufacture. Endotoxins are not as much of an issue for peptides as compared to larger protein complexes. Accordingly, the instant disclosure is directed to a purification process using single use systems in many process steps including high performance chromatography systems thereby removing endotoxins while keeping processing costs low.

8. 发明公开

US20230391823A1 REGENERATION AND MULTIPLE USE OF DEPTH FILTERS 审中-公开
公开(公告)号：US20230391823A1
公开(公告)日：2023-12-07
申请号：US18454131
申请日：2023-08-23
申请人： Hoffmann-La Roche Inc.
发明人： Roberto Falkenstein , Christoph Feistl , Thorsten Lemm , Marc Pompiati , Bernhard Spensberger
IPC分类号： C07K1/34 , B01D37/02 , B01D39/16 , B01D39/18 , B01D39/20
CPC分类号： C07K1/34 , B01D37/025 , B01D39/163 , B01D2239/0407 , B01D39/2079 , B01D2201/085 , B01D39/18
摘要： Herein is reported a method for the purification or production of a therapeutic polypeptide using the same depth filter multiple times, i.e. a depth filter which has been used before and has been regenerated.
Reported herein is a method for purifying or producing a therapeutic polypeptide, characterized in that the method comprises the following steps:

a) filtering an aqueous composition containing said therapeutic polypeptide and impurities through a depth filter, recovering the flow-through and thereby obtaining said purified therapeutic polypeptide,
b) contacting said depth filter with a regeneration solution and thereby regenerating the depth filter, and
c) repeating steps a) and b) one or more times.

9. 发明公开

US20230374063A1 METHODS FOR REDUCING HOST CELL PROTEIN CONTENT IN PROTEIN PURIFICATION PROCESSES 审中-公开
公开(公告)号：US20230374063A1
公开(公告)日：2023-11-23
申请号：US18247084
申请日：2021-10-04
申请人： Eli Lilly and Company
发明人： Brian David Bowes , Lara Ellen Krebs
IPC分类号： C07K1/22 , C07K16/10 , C07K1/34 , C07K1/18 , C07K1/36
CPC分类号： C07K1/22 , C07K16/1003 , C07K1/34 , C07K1/18 , C07K1/36 , C07K2317/14
摘要： The present disclosure relates to methods for reducing host cell protein content in a protein preparation recombinantly produced in a host cell in the manufacturing process of proteins intended for administration to a patient.

10. 发明公开

US20230303622A1 MODELING CONDITIONS FOR TANGENTIAL FLOW FILTRATION PROCESSES FOR PROTEIN PURIFICATION 审中-公开
公开(公告)号：US20230303622A1
公开(公告)日：2023-09-28
申请号：US18023950
申请日：2021-08-30
申请人： Ohio State Innovation Foundation
发明人： Ivan SUSIN PIRES , Andre PALMER
IPC分类号： C07K1/34 , C07K1/16
CPC分类号： C07K1/34 , C07K1/16
摘要： Tangential flow filtration (TFF) is a size-based separation method conventionally used for buffer exchange, concentration, pathogen removal, and for coarse purification. Disclosed herein, TFF was used for selective purification of proteins in their complexed form. In some examples, this process was demonstrated to recover human serum albumin (HSA) in its complexed form from an artificially produced mixture of hemoglobin (Hb) and HSA and from plasma using an anti-HSA polyclonal immunoglobulin G (IgG) as the target-protein binding molecule (TPBM). Moreover, another embodiment of the method recovered haptoglobin (Hp) in its complexed form from human Cohn Fraction IV using Hb as the TPBM. In addition, a mathematical model used to describe the TFF purification process provided that product recovery could be increased without loss of purity by introducing TFF filters with the same MWCO in series. The following disclosure presents a new method for selective purification of proteins using TFF and a simple mathematical model to describe and predict the performance of TFF systems.

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