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1. 发明申请

US20160122723A1 ENGINEERED THREE-DIMENSIONAL SKIN TISSUES, ARRAYS THEREOF, AND METHODS OF MAKING THE SAME 审中-公开
标题翻译：工程三维皮肤组织，其阵列及其制造方法
公开(公告)号：US20160122723A1
公开(公告)日：2016-05-05
申请号：US14933822
申请日：2015-11-05
申请人： ORGANOVO, INC.
发明人： Kelsey Nicole RETTING , Colin M. O'Neill , Deborah Lynn Greene NGUYEN , Sharon C. PRESNELL
IPC分类号： C12N5/071
CPC分类号： A61L27/362 , A61L27/50 , A61L27/60 , A61L2300/64 , C12N5/0698 , C12N2502/091 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , C12N2533/54 , C12N2533/74 , C12N2533/90 , C12N2535/00 , G01N33/5044
摘要： Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.
摘要翻译：公开了生物印迹，三维生物皮肤组织，其包括：包含真皮成纤维细胞的真皮层; 以及表皮层，其包含角质形成细胞，所述表皮层与真皮层接触以形成三维，工程化的生物皮肤组织。还公开了工程化皮肤组织的阵列和制备工程皮肤组织的方法。

2. 发明授权

US08222031B2 Three-dimensional skin model 有权
标题翻译：三维皮肤模型
公开(公告)号：US08222031B2
公开(公告)日：2012-07-17
申请号：US12484810
申请日：2009-06-15
申请人： Michaela Noll , Thomas Graeve
发明人： Michaela Noll , Thomas Graeve
IPC分类号： C12N5/07
CPC分类号： A61L27/3813 , A61K35/12 , A61L27/24 , A61L27/3804 , A61L27/3817 , A61L27/3852 , A61L27/3895 , A61L27/60 , A61L2430/06 , A61L2430/40 , C12N5/0062 , C12N5/0655 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , C12N2533/52 , C12N2533/54 , G01N33/5082
摘要： The present invention relates to methods for cultivating dermal fibroblasts, methods for preparing in vitro dermis equivalents, methods for preparing three-dimensional in vitro skin equivalents, an in vitro dermis equivalent, a three-dimensional in vitro skin equivalent, and methods for determining the effect of a chemical substance or of an agent on human skin cells using the in vitro dermis equivalent and/or the in vitro skin equivalent.
摘要翻译：本发明涉及培养真皮成纤维细胞的方法，体外真皮等效物的制备方法，制备三维体外皮肤当量的方法，体外真皮等效物，三维体外皮肤当量，以及确定使用体外真皮等效和/或体外皮肤当量的化学物质或试剂对人皮肤细胞的作用。

3. 发明授权

US06890754B2 Epidermis/dermis equivalents and aged skin equivalents shaped therefrom 有权
标题翻译：表皮/真皮等效物和由其形成的老化皮肤等效物
公开(公告)号：US06890754B2
公开(公告)日：2005-05-10
申请号：US10659250
申请日：2003-09-11
申请人： Hervé Pageon , Daniel Asselineau , Pierre Tachon
发明人： Hervé Pageon , Daniel Asselineau , Pierre Tachon
IPC分类号： A61L27/00 , A61L27/24 , A61L27/38 , A61L27/60 , C12N5/071 , C12R1/91 , G01N33/50 , C12N5/00 , A61K9/00 , C12N5/08
CPC分类号： A61L27/3886 , A61L27/24 , A61L27/3804 , A61L27/3813 , A61L27/383 , A61L27/60 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , G01N33/5088
摘要： An aged skin equivalent comprising an epidermis equivalent and an aged dermis equivalent, wherein the aged dermis equivalent comprises glycated collagen.An aged dermis equivalent, the epidermis equivalent obtained and methods of producing the aged skin and/or aged dermis equivalent and/or the epidermis equivalent.
摘要翻译：包含表皮当量和老化真皮等效物的老化皮肤当量，其中所述老化真皮等效物包含糖化胶原。老化真皮等效物，所得表皮等效物和老化皮肤和/或老化真皮等效物和/或表皮等效物的生产方法。

4. 发明授权

US06660522B2 Epidermis/dermis equivalents and aged skin equivalents shaped therefrom 有权
标题翻译：表皮/真皮等效物和由其形成的老化皮肤等效物
公开(公告)号：US06660522B2
公开(公告)日：2003-12-09
申请号：US09996905
申请日：2001-11-30
申请人： Hervé Pageon , Daniel Asselineau , Pierre Tachon
发明人： Hervé Pageon , Daniel Asselineau , Pierre Tachon
IPC分类号： C12N508
CPC分类号： A61L27/3886 , A61L27/24 , A61L27/3804 , A61L27/3813 , A61L27/383 , A61L27/60 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , G01N33/5088
摘要： An aged skin equivalent comprising an epidermis equivalent and an aged dermis equivalent, wherein the aged dermis equivalent comprises glycated collagen. An aged dermis equivalent, the epidermis equivalent obtained and methods of producing the aged skin and/or aged dermis equivalent and/or the epidermis equivalent.
摘要翻译：包含表皮当量和老化真皮等效物的老化皮肤当量，其中所述老化真皮等效物包含糖化胶原。老化真皮等效物，所得表皮当量和生产老化皮肤和/或老化真皮等效物和/或表皮的方法当量。

5. 发明申请

US20020142282A1 Immortalized human keratinocyte cell line 有权
标题翻译：永生化人角质形成细胞系
公开(公告)号：US20020142282A1
公开(公告)日：2002-10-03
申请号：US09844194
申请日：2001-04-27
发明人： B. Lynn Allen-Hoffmann
IPC分类号： C12Q001/00 , C12N005/08 , C07H021/04 , C12N015/63
CPC分类号： C12N5/0629 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2502/30 , C12N2503/02 , C12N2503/06 , C12N2510/00 , C12N2510/04 , G01N33/5014 , G01N2500/10
摘要： A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment, this cell invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.
摘要翻译：公开了一种自发永生化的人角质形成细胞系。在一个优选的实施方案中，该细胞系是ATCC 12191.在另一个实施方案中，公开了该细胞发明，测定肿瘤细胞调节剂的作用的方法。该方法包括获得人分层鳞状上皮细胞培养物的步骤，其中培养物包含人恶性鳞状上皮细胞和自发永生化人角质形成细胞，其中培养物形成重构表皮。然后用一种测试的肿瘤细胞调节剂治疗表皮，并评估表皮内恶性细胞的生长。

6. 发明申请

US20020058337A1 Novel epidermis/dermis equivalents and aged skin equivalents shaped therefrom 有权
标题翻译：新表皮/真皮等效物和由其形成的老化皮肤等效物
公开(公告)号：US20020058337A1
公开(公告)日：2002-05-16
申请号：US09996905
申请日：2001-11-30
发明人： Herve Pageon , Daniel Asselineau , Pierre Tachon
IPC分类号： C12N005/08
CPC分类号： A61L27/3886 , A61L27/24 , A61L27/3804 , A61L27/3813 , A61L27/383 , A61L27/60 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , G01N33/5088
摘要： An aged skin equivalent comprising an epidermis equivalent and an aged dermis equivalent, wherein the aged dermis equivalent comprises glycated collagen. An aged dermis equivalent, the epidermis equivalent obtained and methods of producing the aged skin and/or aged dermis equivalent and/or the epidermis equivalent.
摘要翻译：包含表皮当量和老化真皮等效物的老化皮肤当量，其中所述老化真皮等效物包含糖化胶原。老化真皮等效物，所得表皮等效物和老化皮肤和/或老化真皮等效物和/或表皮等效物的生产方法。

7. 发明授权

US6039760A Composite living skin equivalent 失效
标题翻译：复合生皮相当
公开(公告)号：US6039760A
公开(公告)日：2000-03-21
申请号：US602271
申请日：1996-02-16
申请人： Mark Eisenberg
发明人： Mark Eisenberg
IPC分类号： A61K35/36 , A61B10/00 , A61F2/10 , A61K35/12 , A61L27/00 , A61L27/38 , A61L27/60 , C12N5/071 , G01N33/50
CPC分类号： A61L27/3804 , A61F2/105 , A61L27/24 , A61L27/3813 , A61L27/3891 , A61L27/56 , A61L27/60 , C12N5/0698 , G01N33/5005 , G01N33/5014 , A61K35/12 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , C12N2533/54 , Y10S128/08
摘要： Skin equivalents and processes for preparing them are described. The living skin equivalent comprises a layer of cultured keratinocyte cells, a layer of high purity, non-porous collagen and a dermal layer of cultured fibroblast cells in a porous, cross-linked collagen sponge. Processes are described for producing the skin equivalent with descriptions on how to obtain and treat skin precursor materials to yield suitable fibroblasts and keratinocytes.The collagen layers, the physical forms thereof, and treatments thereof are also described. Preferably, the non-porous, highly-purified collagen is selected from Type 1, Type 3, or mixtures of Type 1 and Type 3 collagen. The collagen is purified ideally by treatment with pepsin, to remove antigenic substances.The collagen sponge used can be any suitable form of collagen sponge which will support fibroblast growth.The keratinocytes used in the invention are preferably prepared by the "drop" method spotted evenly on culture media and incubated to coalescence.
摘要翻译：描述了皮肤等同物和制备它们的方法。活体皮肤当量包括一层培养的角质形成细胞，一层高纯度，无孔胶原和培养的成纤维细胞的真皮层，在多孔的交联胶原海绵中。描述了用于生产皮肤等同物的方法，其中描述如何获得和处理皮肤前体材料以产生合适的成纤维细胞和角质形成细胞。还描述了胶原层，其物理形式及其处理。优选地，非多孔，高度纯化的胶原选自1型，3型或1型和3型胶原的混合物。通过用胃蛋白酶处理，理想地纯化胶原蛋白，以去除抗原性物质。使用的胶原海绵可以是支持成纤维细胞生长的任何合适形式的胶原海绵。本发明中使用的角质形成细胞优选通过均匀分布在培养基上的“滴”法进行制备，并孵育至聚结。

8. 再颁专利

USRE35399E Composite living skin equivalents 失效
标题翻译：复合生活皮肤等效
公开(公告)号：USRE35399E
公开(公告)日：1996-12-10
申请号：US346525
申请日：1994-11-29
申请人： Mark Eisenberg
发明人： Mark Eisenberg
IPC分类号： A61K35/36 , A61B10/00 , A61F2/10 , A61K35/12 , A61L27/00 , A61L27/38 , A61L27/60 , C12N5/071 , G01N33/50 , A61F2/02 , A61F2/00 , C12N5/00
CPC分类号： A61L27/3804 , A61F2/105 , A61L27/24 , A61L27/3813 , A61L27/3891 , A61L27/56 , A61L27/60 , C12N5/0698 , G01N33/5005 , G01N33/5014 , A61K35/12 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , C12N2533/54 , Y10S128/08
摘要： A composite living skin equivalent is described comprising an epidermal layer of cultured keratinocyte cells, a layer of non-porous collagen, and a dermal layer of cultured fibroblast cells in a porous cross-linked collagen sponge matrix. Preferably the non-porous collagen is Type 1, Type 3 or mixtures of Types 1 and 3 bovine collagen, which has been pepsin treated. A process for preparing the skin equivalent is described, as well as a test kit for in vitro testing of the skin equivalent. The skin equivalent has use for skin grafting as well as in vitro testing of the effects of various substances on skin.The questions raised in reexamination request No. 90/003,418, filed Apr. 25, 1994, have been considered and the results thereof are reflected in this reissue patent which constitutes the reexamination certificate required by 35 U.S.C. 307 as provided in 37 CFR 1.570(e).
摘要翻译： PCT No.PCT / AU91 / 00160 Sec。 371日期1991年11月27日 102（e）日期1991年11月27日PCT 1991年4月24日PCT PCT。公开号WO91 / 16010PC 日期1991年10月31日描述了一种复合活体皮肤等效物，其包含培养的角质形成细胞的表皮层，无孔胶原层和培养的成纤维细胞的真皮层，多孔交联胶原海绵基质。优选地，无孔胶原蛋白是已被胃蛋白酶处理的1型，3型或1型和3型牛胶原蛋白的混合物。描述了制备皮肤等效物的方法，以及用于体外测试皮肤当量的测试试剂盒。皮肤等效物可用于皮肤移植以及体外测试各种物质对皮肤的影响。 + RR已经考虑了1994年4月25日提交的第90 / 003,418号复审请求中提出的问题，其结果反映在该重新颁发专利中，该专利构成了35U.S.C.所要求的复审证书。 307如第37 CFR 1.570（e）条所规定。

9. 发明授权

US5282859A Composite living skin equivalents 失效
标题翻译：复合生活皮肤等效
公开(公告)号：US5282859A
公开(公告)日：1994-02-01
申请号：US777419
申请日：1991-11-27
申请人： Mark Eisenberg
发明人： Mark Eisenberg
IPC分类号： A61K35/36 , A61B10/00 , A61F2/10 , A61K35/12 , A61L27/00 , A61L27/38 , A61L27/60 , C12N5/071 , G01N33/50 , A61F2/02 , A61F2/00 , C12N5/00
CPC分类号： A61L27/3804 , A61F2/105 , A61L27/24 , A61L27/3813 , A61L27/3891 , A61L27/56 , A61L27/60 , C12N5/0698 , G01N33/5005 , G01N33/5014 , A61K35/12 , C12N2502/094 , C12N2502/1323 , C12N2503/06 , C12N2533/54 , Y10S128/08
摘要： A composite living skin equivalent is described comprising an epidermal layer of cultured keratinocyte cells, a layer of non-porous collagen, and a dermal layer of cultured fibroblast cells in a porous cross-linked collagen sponge matrix. Preferably the non-porous collagen is Type 1, Type 3 or mixtures of Types 1 and 3 bovine collagen, which has been pepsin treated. A process for preparing the skin equivalent is described, as well as a test kit for in vitro testing of the skin equivalent. The skin equivalent has use for skin grafting as well as in vitro testing of the effects of various substances on skin.
摘要翻译： PCT No.PCT / AU91 / 00160 Sec。 371日期1991年11月27日 102（e）日期1991年11月27日PCT 1991年4月24日PCT PCT。公开号WO91 / 16010PC 1991年10月31日的日期。描述了一种复合活体皮肤等效物，其包含培养的角质形成细胞的表皮层，无孔胶原层和培养的成纤维细胞的真皮层在多孔交联胶原海绵基质中。优选地，无孔胶原蛋白是已被胃蛋白酶处理的1型，3型或1型和3型牛胶原蛋白的混合物。描述了制备皮肤等效物的方法，以及用于体外测试皮肤当量的测试试剂盒。皮肤等效物可用于皮肤移植以及体外测试各种物质对皮肤的影响。

10. 发明授权

US12061189B2 Models of reconstructed sensitive skin 有权
公开(公告)号：US12061189B2
公开(公告)日：2024-08-13
申请号：US16624087
申请日：2018-06-21
申请人： Laboratoires Expanscience
发明人： Gaelle Bellemere , Stephanie Bredif , Gaetan Boyer , Caroline Baudouin
IPC分类号： G01N33/50 , C12N5/071
CPC分类号： G01N33/5088 , C12N5/0698 , C12N2503/06
摘要： The present invention relates to models of reconstructed sensitive skin reproducing the features of sensitive skin, as well as to processes for obtaining such models. The present invention further relates to in vitro processes for testing formulations or active ingredients for the prevention or treatment of sensitive skin.

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